Evidence against the Presence of H2 Histocompatibility Antigens in Ehrlich Ascites Tumour Cells

Abstract

THE use of a hybrid between Ehrlich ascites tumour and hamster cell lines to immunize mice against the tumour has been reported¹. We report here the use for the same purpose of a hybrid line between Ehrlich ascites tumour cells and cells derived from mouse embryo cells obtained from an inbred strain of mice (CBA). This hybrid is less immunologically foreign than the hamster × Ehrlich hybrid for the randomly bred Swiss mice used in the immunization experiments; it differs antigenically only in histocompatibility antigens, in particular in its H2 antigen, which, for CBA mice, is the H2^k antigen. Such a hybrid gave the opportunity of investigating whether the immunity arising after injection of Ehrlich × hamster hybrids¹ was the result of strong histocompatibility antigens (of H2 type) in the Ehrlich cells.