Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

A New Consistent Chromosomal Abnormality in Chronic Myelogenous Leukaemia identified by Quinacrine Fluorescence and Giemsa Staining

Abstract

CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Purchase on Springer Link

Instant access to full article PDF

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Chicago Conference. Standardization in human cytogenetics. The National Foundation—March of Dimes (1966).

  2. Paris Conference. Standardization in human cytogenetics. The National Foundation—March of Dimes (1972).

  3. Rowley, J. D., Blaisdell, R. K., and Jacobson, L. O., Blood, 27, 782 (1966).

    CAS  PubMed  Google Scholar 

  4. Rowley, J. D., Potter, D., and Mikuta, J., Stain Tech., 46, 97 (1971).

    CAS  Google Scholar 

  5. Pearson, P. L., Bobrow, M., and Vosa, C. G., Nature, 226, 78 (1970).

    Article  ADS  CAS  Google Scholar 

  6. Sumner, A. T., Evans, H. J., and Buckland, R. A., Nature New Biology, 232, 31 (1971).

    Article  CAS  Google Scholar 

  7. Rowley, J. D., and Bodmer, W. F., Nature, 231, 503 (1971).

    Article  ADS  CAS  Google Scholar 

  8. Caspersson, T., Gahrton, G., Lindsten, J., and Zech, L., Exp. Cell Res., 63, 238 (1970).

    Article  CAS  Google Scholar 

  9. O'Riordan, M. L., Robinson, J. A., Buckton, K. E., and Evans, H. J., Nature, 230, 167 (1971).

    Article  ADS  CAS  Google Scholar 

  10. Rudkin, G. T., and Hungerford, D. A., Science, NY, 144, 1229 (1964).

    Article  ADS  CAS  Google Scholar 

  11. Whang-Peng, J., Canellos, G. P., Carbone, P. P., and Tjio, J. H., Blood, 32, 755 (1968).

    CAS  PubMed  Google Scholar 

  12. Ezdinli, E. Z., Sokal, J. E., Crosswhite, B. S., and Sandberg, A. A., Ann. Int. Med., 72, 175 (1970).

    Article  CAS  Google Scholar 

  13. Lobb, D. S., Reeves, B. R., and Lawler, S. D., Lancet, i 849 (1972).

  14. Rowley, J. D., Ann. Génét. (in the press).

  15. Manolov, G., and Manolova, Y., Hereditas, 69, 300 (1971).

    Google Scholar 

  16. Mark, J., Levan, G., and Mitelman, F., Hereditas, 71, 163 (1972).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

ROWLEY, J. A New Consistent Chromosomal Abnormality in Chronic Myelogenous Leukaemia identified by Quinacrine Fluorescence and Giemsa Staining. Nature 243, 290–293 (1973). https://doi.org/10.1038/243290a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/243290a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing