Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Infection and Transformation of Bovine Lymphoid Cells in vitro by Infective Particles of Theileria parva

Nature volume 245, pages 101–103 (1973)Cite this article

Abstract

TECHNIQUES recently developed in this laboratory can be used to infect cattle reproducibly with East Coast fever with infective particles of Theileria parva collected from the tick vector, Rhipicephalus appendiculatus. Infective particles can be collected either as in vitro tick feed material by inducing prefed, infected ticks to salivate into capillary tubes containing foetal calf serum1, or as ground tick supernatant obtained by grinding the ticks in Eagle's minimum essential medium with Earle's salts (MEM) supplemented with bovine plasma albumin (Fraction V from bovine plasma) (BPA) and collecting the supernatant2.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Cunningham, M. P., Brown, C. G. D., Burridge, M. J., Musoke, A. J., Purnell, R. E., Radley, D. E., and Sempebwa, C. (in the press).

  2. Purnell, R. E., Brown, C. G. D., Cunningham, M. P., Kirimi, I. M., and Ledger, M. A. (in the press).

  3. Malmquist, W. A., Nyindo, M. B. A., and Brown, C. G. D., Trop. Anim. Hlth Prod., 2, 139 (1970).

    Article  Google Scholar 

  4. Aalund, O., Hoerlein, A. B., and Adler, H. C., Acta vet. scand., 11, 331 (1970).

    CAS  PubMed  Google Scholar 

  5. Moulton, J. E., Krauss, H. H., and Malmquist, W. A., Lab. Invest., 24, 187 (1971).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. UNDP/FAO Tick-borne Diseases Project, East African Veterinary Research Organization Muguga, PO Box 32, Kikuyu

    C. G. D. BROWN, D. A. STAGG, R. E. PURNELL, G. K. KANHAI & R. C. PAYNE

  2. On ODA secondment from MAFF Central Veterinary Laboratory, Weybridge, Surrey

    D. A. STAGG & R. C. PAYNE

  3. On ODA secondment from ARC Institute for Research on Animal Diseases, Compton, Berkshire

    R. E. PURNELL

Authors
  1. C. G. D. BROWN
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. A. STAGG
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. E. PURNELL
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. K. KANHAI
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. C. PAYNE
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

BROWN, C., STAGG, D., PURNELL, R. et al. Infection and Transformation of Bovine Lymphoid Cells in vitro by Infective Particles of Theileria parva. Nature 245, 101–103 (1973). https://doi.org/10.1038/245101a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/245101a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing