Abstract
A NUCLEOSIDE triphosphatase activity showing broad substrate specificity has been found in nuclear envelope isolated from rat and pig liver, Tetrahymena macronucleus, and cultured SV-3T3 fibroblasts using the method of Harris and Milne1, and has been extensively characterised in this laboratory. There is evidence that in purified nuclear envelopes there is only one enzyme which catalyses nucleoside triphosphate hydrolysis: the activities observed with different substrates and metal ions are not additive, and they show a single pH optimum, the value of which varies with the source of the envelopes2. This enzyme seems to be localised in the nuclear pore complexes3. Several reports4–13 have indicated that ATP markedly stimulates the release of mRNA from isolated nuclei, but no evidence has been presented to suggest that this stimulation necessarily requires hydrolysis of the ATP. In this paper, however, we present evidence that the nucleoside triphosphatase is an essential component of the mechanism of nucleocytoplasmic translocation of ribonucleoprotein.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Harris, J. R., and Milne, J. F., Biochem. Soc. Trans., 2, 1251–1253 (1974).
Agutter, P. S., et al., Biochim. biophys. Acta (in the press).
Franke, W. W., Int. Rev. Cytol. suppl., 4, 71–236 (1974).
Ishikawa, K., Kuroda, C., and Ogata, K., Biochim. biophys. Acta, 179, 316–331 (1969).
Ishikawa, K., Kuroda, C., Ueki, M., and Ogata, K., Biochim. biophys. Acta, 213, 495–504 (1970).
Ishikawa, K., Kuroda, C., and Ogata, K., Biochim. biophys. Acta, 213, 505–512 (1970).
Raskas, H. J., Nature new Biol., 233, 134–136 (1971).
Raskas, H. J., and Bhaduri, S., Biochemistry, 12, 920–925 (1973).
Schumm, D. E., and Webb, T. E., Biochem. biophys. Res. Commun., 48, 1259–1265 (1972).
Yu Ling, C., Racevskis, J., and Webb, T. E., Cancer Res., 32, 2314–2321 (1972).
Schumm, D. E., McNamara, D. S., and Webb, T. E., Nature new Biol., 245, 201–203 (1973).
Schumm, D. E., Morris, H., and Webb, T. E., Cancer Res., 33, 1821–1828 (1973).
McNamara, D. S., Racevskis, J., Schumm, D. E., and Webb, T. E., Biochem. J., 147, 193–197 (1975).
Stenram, U., and Willem, R., Expl Cell Res., 50, 505–514 (1968).
Wohler, B. E., and Wollenberger, A., Biochim. Z., 329, 508–520 (1958).
Schumm, D. E., and Webb, T. E., Biochem. biophys. Res. Commun., 58, 354–360 (1974).
Ishikawa, K., Ueki, M., Nagai, K., and Ogata, K., Biochim. biophys. Acta, 259, 138–154 (1972).
Agutter, P. S., Cockrill, J. B., Harris, J. R., Vincent, C. A., and Wolfe, M. A., Biochim. biophys. Acta (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
AGUTTER, P., MCARDLE, H. & MCCALDIN, B. Evidence for involvement of nuclear envelope nucleoside triphosphatase in nucleocytoplasmic translocation of ribonucleoprotein. Nature 263, 165–167 (1976). https://doi.org/10.1038/263165a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/263165a0
This article is cited by
-
A binding protein for fungal signal molecules in the cell wall of Pisum sativum
Journal of General Plant Pathology (2006)
-
Effect of tryptophan on rat hepatic nuclear poly(A)polymerase activity
Amino Acids (1993)
-
Translational regulation in rat brain hemispheres
Neurochemical Research (1987)
-
Nucleocytoplasmic RNA transport
Molecular and Cellular Biochemistry (1985)
-
Modulation of RNA transport by polyvinylpyrrolidone
Molecular Biology Reports (1984)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
