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Antibodies to major histocompatibility antigens produced by hybrid cell lines

Nature volume 266, pages 550–552 (1977)Cite this article

Abstract

FUSION between myeloma cells and spleen cells from immunised donors has been shown to be a successful method of deriving homogeneous anti-SRBC (anti-sheep red blood cell) and anti-TNP antibodies1,2. One of the most powerful features of this approach is that, by cloning, one may easily derive cell lines synthesising monoclonal antibodies despite using non-purified immunogens. The multiple components of a heterogeneous population of hybrid cells are resolved by cloning techniques. This feature makes the system a very powerful tool in the study of complex antigenic structures. The established cell lines offer the further advantage of unlimited permanent supply of material, and the possibility of worldwide standardisation.

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Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge

    G. GALFRE, S. C. HOWE & C. MILSTEIN

  2. On leave of absence from the Department of Medical Genetics, University of Turin, Turin, Italy

    G. GALFRE

  3. ARC Institute of Animal Physiology, Babraham, Cambridge, UK

    G. W. BUTCHER & J. C. HOWARD

Authors
  1. G. GALFRE
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  2. S. C. HOWE
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  3. C. MILSTEIN
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  4. G. W. BUTCHER
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  5. J. C. HOWARD
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GALFRE, G., HOWE, S., MILSTEIN, C. et al. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature 266, 550–552 (1977). https://doi.org/10.1038/266550a0

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