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Synchronised transmembrane insertion and glycosylation of a nascent membrane protein

Nature volume 269, pages 775–780 (1977)Cite this article

Abstract

Studies of the synthesis and incorporation of the vesicular stomatitis virus glycoprotein into membranes in a synchronised cell-free system demonstrate a tight coupling between polypeptide synthesis and membrane insertion, as a result of which the nascent chain crosses the membrane. The studies reveal a surprisingly precise sequence by which the nascent chain of this membrane glycoprotein is glycosylated in two steps. These findings have important implications for the mechanisms of membrane assembly.

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Authors and Affiliations

  1. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139

    James E. Rothman & Harvey F. Lodish

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  1. James E. Rothman
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  2. Harvey F. Lodish
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Rothman, J., Lodish, H. Synchronised transmembrane insertion and glycosylation of a nascent membrane protein. Nature 269, 775–780 (1977). https://doi.org/10.1038/269775a0

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