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Expression in Escherichia coli of hepatitis B virus DNA sequences cloned in plasmid pBR322

Nature volume 279, pages 43–47 (1979)Cite this article

Abstract

Fragments of hepatitis B virus DNA isolated from Dane particles have been inserted into the Escherichia coli plasmid pBR322 and cloned. Cells carrying the hybrid plasmid synthesise antigenic material that reacts specifically with antisera to hepatitis B viral antigens.

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Author notes

  1. C. J. Burrell

    Present address: Division of Virology, I.M.V.S., Box 14, Rundle St. Post Office, Adelaide, S. Australia

Authors and Affiliations

  1. Department of Bacteriology, University of Edinburgh Medical School, Edinburgh, UK

    C. J. Burrell & Patricia Mackay

  2. Microbiological Research Establishment, Porton Down, Salisbury, Wiltshire, UK

    P. J. Greenaway & P. H. Hofschneider

  3. Department of Molecular Biology, University of Edinburgh, King's Buildings, Edinburgh, UK

    K. Murray

  4. Max Planck Institute for Biochemistry, Am Klopferspitz, Martinsried bei Munich, FRG

    P. H. Hofschneider

Authors
  1. C. J. Burrell
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  2. Patricia Mackay
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  3. P. J. Greenaway
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  4. P. H. Hofschneider
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  5. K. Murray
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Burrell, C., Mackay, P., Greenaway, P. et al. Expression in Escherichia coli of hepatitis B virus DNA sequences cloned in plasmid pBR322. Nature 279, 43–47 (1979). https://doi.org/10.1038/279043a0

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