Abstract
A composite DNA sequence of regions of hepatitis B virus, determined from a series of recombinant plasmids, reveals the genes for the surface antigen and the core antigen of the virus. The sequence of the core antigen shows it to be a DNA binding protein. The core antigen gene is expressed in Escherichia coli and when injected into rabbits the bacterial product induces antibodies which react with core antigen isolated from human sources.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Fritsch, A., Pourcel, C., Charnay, P. & Tiollais, P. C. r. hebd. Séanc. Acad. Sci., Paris, Ser. D 287, 1453–1456 (1978).
Charnay, P., Pourcel, C., Louise, A., Fritsch, A. & Tiollais, P. Proc. natn. Acad. Sci. U.S.A. 76, 2222–2226 (1979).
Burrell, C. J., Mackay, P., Greenaway, P. J., Hofschneider, P. H. & Murray, K. Nature 279, 43–47 (1979).
Sninsky, J. J., Siddiqui, A., Robinson, W. S. & Cohen, S. N. Nature 279, 346–348 (1979).
Villa-Komaroff, L. et al. Proc. natn. Acad. Sci. U.S.A. 75, 3727–3731 (1978).
Robinson, W. S. A. Rev. Microbiol. 31, 357–377 (1977).
Gerin, J. L. & Shih, J. W.-K. in Viral Hepatitis (eds Vyas, G. N., Cohen, S. N. & Schmid, R.) Ch. 13 (Franklin Institute, Philadelphia, 1978).
Peterson, D. L., Roberts, I. M. & Vyas, G. N. Proc. natn. Acad. Sci. U.S.A. 74, 1530–1534 (1977).
Broome, S. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 75, 2746–2749 (1978).
Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977); Meth. Enzym. 65 (in the press).
Nakano, M., Tobita, T. & Ando, T. Int. J. Peptide Protein Res. 8, 565–578 (1976).
Lomedico, P. et al. Cell (in the press).
Hagenbuchle, O., Santer, M. & Steitz, J. A. Cell 13, 551–563 (1978).
Ziff, E. B. & Evans, R. M. Cell 15, 1463–1475 (1978).
Ouchterlony, O. in Handbook of Experimental Immunology (ed. Weir, D. W.) Ch. 19 (Blackwell, Oxford, 1967).
Cohen, B. J. & Cossart, Y. E. J. clin. Path. 30, 709–713 (1977).
Spiro, R. G. Adv. Protein Chem. 27, 349–467 (1973).
Kornberg, A. in DNA Synthesis (Freeman, San Francisco, 1974).
Chalberg, M. & Englund, P. T. J. biol. Chem. 254, 7812–7819 (1979).
Takahashi, K. et al. J. Immun. 122, 275–279 (1979).
Peterson, D. L., Chien, D. Y., Vyas, G. N., Nitecki, D. & Bond, H. E. in Viral Hepatitis (eds Vyas, G. N., Cohen, S. N. & Schmid, R.) Ch. 54 (Franklin Institute, Philadelphia, 1978).
Valenzuela, P. et al. Nature 280, 815–819 (1979).
Charnay, P. et al. Nucleic Acids Res. 7, 335–346 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pasek, M., Goto, T., Gilbert, W. et al. Hepatitis B virus genes and their expression in E. coli. Nature 282, 575–579 (1979). https://doi.org/10.1038/282575a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/282575a0
This article is cited by
-
Aspects of Biological Replication and Evolution Independent of the Central Dogma: Insights from Protein-Free Vesicular Transformations and Protein-Mediated Membrane Remodeling
The Journal of Membrane Biology (2022)
-
Peculiarities in the designations of hepatitis B virus genes, their products, and their antigenic specificities: a potential source of misunderstandings
Virus Genes (2020)
-
Effects of mRNA secondary structure on the expression of HEV ORF2 proteins in Escherichia coli
Microbial Cell Factories (2017)
-
Adaptive evolution of proteins in hepatitis B virus during divergence of genotypes
Scientific Reports (2017)
-
Hepatocytic expression of human sodium-taurocholate cotransporting polypeptide enables hepatitis B virus infection of macaques
Nature Communications (2017)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.