Abstract
Mutations in the v gene of bacteriophage T4 are associated with a marked increase in sensitivity to killing by UV radiation at 254 nm, but not to a variety of other forms of base damage to DNA1–4. Early studies from this laboratory provided evidence for a role of the v gene in the excision of pyrimidine dimers (PD) from DNA. Specifically, it was shown that extracts of T4v+-infected Esherichia coli catalyse the formation of single-strand breaks (nicks) and/or alkali-labile sites in UV-irradiated duplex DNA5. Comparable hydrolysis of phosphodiester bonds is not observed with extracts of E. coli infected with the mutant T4v1, (ref. 5). The product of the v gene has been extensively purified in a number of laboratories6–9; however, convincing evidence of purification to physical homogeneity has not yet been presented.
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References
Harm, W. Virology 19, 66–71 (1963).
Friedberg, E. C. Mutat. Res. 15, 113–123 (1972).
Ito, I. & Sekiguichi, M. Jap. J. Genet. 51, 29–33 (1976).
Nishida, Y., Yasuda, S. & Sekiguchi, M. Biochim. biophys. Acta 442, 208–215 (1976).
Friedberg, E. C. & King, J. J. Biochem. biophys. Res. Commun. 37, 646–651 (1969).
Yasuda, S. & Sekiguchi, M. Proc. natn. Acad. Sci. U.S.A. 67, 1839–1845 (1970).
Friedberg, E. C. & King, J. J. J. Bact. 106, 500–507 (1971).
Yasuda, S. & Sekiguchi, M. Biochim. biophys. Acta 442, 197–207 (1976).
Friedberg, E. C., Ganesan, A. K. & Seawell, P. C. Meth. Enzym. (in the press).
Friedberg, E. C. & Lehman, I. R. Biochem. biophys. Res. Commun. 58, 132–139 (1974).
Minton, K., Durphy, M., Taylor, R. & Friedberg, E. C. J. biol. Chem. 250, 2823–2829 (1975).
Friedberg, E. C. Photochem. Photobiol. 21, 277–289 (1975).
Grossman, L., Riazuddin, S., Haseltine, W. A. & Lindan, C. Cold Spring Harb. Symp. Quant. Biol. 43, 947–955 (1979).
Haseltine, W. A. et al. Nature (in the press).
Riazuddin, S. & Grossman, L. J. biol. Chem. 252, 6287–6293 (1977).
Setlow, R. B. Science 153, 379–386 (1966).
Setlow, R. B. Prog. nucleic Acid. Res. molec. Biol 8, 257–295 (1968).
Rhan, R. O. & Landry, L. C. Photochem. Photobiol. 18, 29–38 (1973).
Friedberg, E. C. & Clayton, D. A. Nature 237, 99 (1972).
Ganesan, A. K. J. molec. Biol. 87, 103–119 (1974).
Ganesan, A. K., Smith, C. A. & Van Zeeland, A. A. in DNA Repair: A Laboratory Manual of Research Procedures (eds Friedberg, E. C. & Hanawalt, P. C.) (Dekker, New York, in the press).
Cook, K. H. & Friedberg, E. C. Analyt. Biochem. 73, 411–418 (1976).
Reynolds, R. J. Mutat. Res. 50, 43–56 (1978).
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Radany, E., Friedberg, E. A pyrimidine dimer–DNA glycosylase activity associated with the v gene product of bacteriophage T4. Nature 286, 182–185 (1980). https://doi.org/10.1038/286182a0
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DOI: https://doi.org/10.1038/286182a0
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