Abstract
Many primary products of transcription in eukaryotes contain sequences that are removed during maturation of mRNAs, although the structural determinants that direct this highly specific splicing reaction are unknown. Comparative sequence studies of known splice points1–4 have identified a short consensus sequence to which all known splice points conform to varying degrees. However, there is no direct evidence that this consensus sequence is necessary for splicing to occur. In this communication, an adenovirus mutant is described which contains a cis dominant defect in the splicing of RNA from one of the early transcriptional units. The defect is ascribed to changes in two adjacent nucleotides within a splice site consensus sequence. The mutations lie in a region of the adenovirus genome (the transforming region 1A) which normally produces a set of three co-terminal mRNAs differing only by the internal sequences removed during post-transcriptional processing. The mutant has thus been used not only to define sequences required for splicing, but also to investigate the pathways along which a series of related mRNAs is generated.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Breathnach, R., Benoist, C., O'Hare, K., Gannon, F. & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 75, 4853–4857 (1978).
Seif, I., Khoury, G. & Dhar, R. Nucleic Acids Res. 6, 3387–3398 (1979).
Lerner, M. R., Boyle, J. A., Mount, S. M., Wolin, S. L. & Steitz, J. A. Nature 283, 220–224 (1980).
Rogers, J. & Wall, R. Proc. natn. Acad. Sci. U.S.A. 77, 1877–1879 (1980).
Graham, F. L., Smiley, J., Russell, W. C. & Nairn, R. J. gen. Virol. 36, 59–72 (1977).
Aiello, L., Huebner, K. & Weinmann, R. Virology 94, 460–469 (1979).
Berk, A. J., Lee, F., Harrison, T., Williams, J. & Sharp, P. A. Cell 17, 935–944 (1979).
Frost, E. & Williams, J. Virology 91, 39–50 (1978).
Jones, N. & Shenk, T. Cell 17, 683–689 (1979).
Williams, J. F., Gharpure, M., Ustacelebi, S. & McDonald, S. J. gen. Virol. 11, 95–101 (1971).
Berk, A. J. & Sharp, P. A. Cell 12, 721–732 (1977).
Berk, A. J. & Sharp, P. A. Cell 14, 695–711 (1978).
Ross, S. R., Levine, A. J., Galos, R. S., Williams, J. & Shenk, T. Virology 103, 475–492 (1980).
Salser, W. Cold Spring Harb. Symp. quant. Biol. 42, 985–1002 (1977).
Lai, C.-J. & Nathans, D. Virology 60, 466–475 (1974).
Hamer, D. A. & Leder, P. Cell 17, 737–747 (1979).
Khoury, G., Gruss, P., Dhar, R. & Lai, C.-J. Cell 18, 85–92 (1979).
Chow, L. T., Broker, T. R. & Lewis, J. B. J. molec. Biol. 134, 265–303 (1979).
Kitchingman, G. R. & Westphal, H. J. molec. Biol. 137, 23–48 (1980).
Perricaudet, M., Le Moullec, J. M. & Pettersson, U. Proc. natn. Acad. Sci. U.S.A. 77, 3778–3782 (1980).
Berk, A. J. & Sharp, P. A. Proc. natn. Acad. Sci. U.S.A. 75, 1274–1278 (1978).
Reddy, V. B., Ghosh, P. K., Lebowitz, P., Piatak, M. & Weissman, S. M. J. Virol. 30, 279–296 (1979).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Maniatis, T., Kee, S. G., Efstradiatis, A. & Kafatos, F. C. Cell 8, 163–182 (1976).
Sambrook, J. et al. Cold Spring Harb. Symp. quant. Biol. 44, 569–584 (1980).
Reddy, R., Ro-Choi, T. S., Henning, D. & Busch, H. J. biol. Chem. 249, 6486–6494 (1974).
van Ormondt, H., Maat, J., De Waard, A. & Van der Eb, A. J. Gene 4, 309–328 (1978).
Maxam, A. & Gilbert, W. in Meth. Enzym. 65, 499–560 (1980).
Thimmappaya, B. & Shenk, T. J. Virol. 30, 668–673 (1979).
Volckaert, G., Feunteun, J., Crawford, L., Berg, P. & Fiers, W. J. Virol. 30, 674–682 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Solnick, D. An adenovirus mutant defective in splicing RNA from early region 1A. Nature 291, 508–510 (1981). https://doi.org/10.1038/291508a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/291508a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
