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Immunoglobulin heavy-chain expression and class switching in a murine leukaemia cell line

Nature volume 296, pages 325–331 (1982)Cite this article

A Corrigendum to this article was published on 01 April 1982

Abstract

A cell line that switches from μ to γ2b synthesis during growth in culture uses the same VH region for both heavy chains but retains two copies of the Cμ gene. This suggests that the μ to γ2b class switch can occur, at least in part, by an RNA processing mechanism. Regulatory variants of this cell line lose constitutive μ-chain synthesis but simultaneously acquire lipopolysaccharide(LPS)-inducible synthesis of that chain. This co-variation is allele-specific and is correlated to a large deletion of DNA in the JH–Cμ intron.

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Author notes

  1. Frederick W. Alt

    Present address: Department of Biochemistry, College of Physicians and Surgeons, Columbia University, New York, New York, 10027, USA

  2. Naomi Rosenberg and Rose J. Casanova: Cancer Research Center, Tufts University School of Medicine, Boston, Massachusetts 02111, USA

Authors and Affiliations

  1. Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    Frederick W. Alt, Naomi Rosenberg, Rose J. Casanova, Elise Thomas & David Baltimore

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  1. Frederick W. Alt
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  2. Naomi Rosenberg
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  5. David Baltimore
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Alt, F., Rosenberg, N., Casanova, R. et al. Immunoglobulin heavy-chain expression and class switching in a murine leukaemia cell line. Nature 296, 325–331 (1982). https://doi.org/10.1038/296325a0

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