Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Sequence requirements for nuclear location of simian virus 40 large-T antigen

Nature volume 311, pages 33–38 (1984)Cite this article

Abstract

A point mutation in the simian virus 40 large-T gene, which was generated by mixed oligonucleotide mutagenesis and resulted in the conversion of Lys 128 to Thr, produced a large-T antigen that was detected in the cytoplasm but not the nucleus of cells. Deletions within the surrounding sequence Lys-128Lys-Lys-Arg-Lys-Val-Glu also produce cytoplasmic large-T and define a region of the protein involved in nuclear location.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Tooze, J. Molecular Biology of Tumor Viruses Part 2, 2nd edn (Cold Spring Harbor Laboratory, New York, 1981).

    Google Scholar 

  2. Tegtmeyer, P. J. Virol. 10, 591–598 (1972).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Pope, J. H. & Rowe, W. P. J. exp. Med. 120, 121–128 (1964).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Santos, M. & Butel, J. S. Virology 120, 1–17 (1982).

    Article  CAS  PubMed  Google Scholar 

  5. Klockmann, U. & Deppert, W. EMBO J. 2, 1151–1157 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Tjian, R. Cell 13, 165–179 (1978).

    Article  CAS  PubMed  Google Scholar 

  7. Shortle, D. R., Margolskee, R. F. & Nathans, D. Proc. natn. Acad. Sci. U.S.A. 76, 6128–6131 (1979).

    Article  ADS  CAS  Google Scholar 

  8. Rio, D., Robbins, A., Myers, R. & Tjian, R. Proc. natn. Acad. Sci. U.S.A. 77, 5706–5710 (1980).

    Article  ADS  CAS  Google Scholar 

  9. Kalderon, D. & Smith, A. E. Virology (in the press).

  10. Prives, C., Beck, Y. & Shure, H. J. Virol. 33, 689–696 (1980).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Chaudry, F., Harvey, R. & Smith, A. E. J. Virol. 44, 54–66 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Clark, C., Peden, K., Pipas, J. M., Nathans, D. & Tjian, R. Molec. cell. Biol. 3, 220–228 (1983).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Morrisson, B., Kress, M., Khoury, G. & Jay, G. J. Virol. 47, 106–114 (1983).

    Google Scholar 

  14. Scheidtmann, K. H., Echle, B. & Walter, G. J. Virol. 44, 116–133 (1981).

    Google Scholar 

  15. Hutchinson, C. A. III et al. J. biol. Chem. 253, 6551–6560 (1978).

    Google Scholar 

  16. Smith, M. & Gillam, S. in Genetic Engineering, Principles and Methods, Vol. 3 (eds Setlow, J. K. & Hollaender, A.) (Plenum, New York, 1981).

    Google Scholar 

  17. Grunstein, M. & Hogness, D. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).

    Article  ADS  CAS  Google Scholar 

  18. Wallace, R. B. et al. Nucleic Acids Res. 6, 3543–3557 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Wallace, R. B. et al. Gene 16, 21–26 (1981).

    Article  CAS  PubMed  Google Scholar 

  20. Graham, F. L. & van der Eb, A. J. Virology 52, 456–467 (1973).

    Article  CAS  PubMed  Google Scholar 

  21. Harlow, E., Crawford, L. V., Pim, D. C. & Williamson, N. M. J. Virol. 39, 861–869 (1981).

    CAS  PubMed  PubMed Central  Google Scholar 

  22. Richardson, W. D. & Westphal, H. Cell 27, 133–141 (1981).

    Article  CAS  PubMed  Google Scholar 

  23. Kalderon, D., Oostra, B. A., Ely, B. K. & Smith, A. E. Nucleic Acids Res. 10, 5161–5171 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Myers, R. M. & Tjian, R. Proc. natn. Acad. Sci. U.S.A. 77, 6491–6495 (1980).

    Article  ADS  CAS  Google Scholar 

  25. Dulbecco, R. & Freeman, G. Virology 8, 396–397 (1959).

    Article  CAS  PubMed  Google Scholar 

  26. McKay, R. D. G. J. molec. Biol. 145, 471–488 (1981).

    Article  CAS  PubMed  Google Scholar 

  27. Rapp, F., Pauluzzi, S. & Butel, J. S. J. Virol. 4, 626–631 (1969).

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Pollack, R., Risser, R., Conlon, S. & Rifkin, D. Proc. natn. Acad. Sci. U.S.A. 71, 4792–4796 (1974).

    Article  ADS  CAS  Google Scholar 

  29. Risser, R. & Pollack, R. Virology 59, 477–489 (1974).

    Article  CAS  PubMed  Google Scholar 

  30. Lanford, R. G. & Butel, J. S. Virology 110, 147–158 (1981).

    Article  CAS  PubMed  Google Scholar 

  31. Land, H., Parada, L. F. & Weinberg, R. A. Science 222, 771–778 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  32. Paine, P. L. & Feldherr, C. M. Expl Cell Res. 74, 81–98 (1972).

    Article  CAS  Google Scholar 

  33. Paine, P. L., Moore, L. C. & Horowitz, S. B. Nature 254, 109–114 (1975).

    Article  ADS  CAS  PubMed  Google Scholar 

  34. De Robertis, E. M. Cell 32, 1021–1025 (1983).

    Article  CAS  PubMed  Google Scholar 

  35. Dingwall, C., Sharnick, S. V. & Laskey, R. A. Cell 30, 449–458 (1982).

    Article  CAS  PubMed  Google Scholar 

  36. Hall, M. N., Hereford, L. & Herskowitz, I. Cell 36, 1057–1065 (1984).

    Article  CAS  PubMed  Google Scholar 

  37. Prentki, P., Karen, F., Iida, S. & Meyer, J. Gene 14, 289–299 (1981).

    Article  CAS  PubMed  Google Scholar 

  38. Edge, M. D. et al. Nature 292, 756–761 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Alexander F. Markham: ICI Pharmaceuticals Division, Alderley Park, Macclesfield, Cheshire SK10 4TG, UK

Authors and Affiliations

  1. Biochemistry Division, National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK

    Daniel Kalderon, William D. Richardson, Alexander F. Markham & Alan E. Smith

Authors
  1. Daniel Kalderon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. William D. Richardson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alexander F. Markham
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alan E. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kalderon, D., Richardson, W., Markham, A. et al. Sequence requirements for nuclear location of simian virus 40 large-T antigen. Nature 311, 33–38 (1984). https://doi.org/10.1038/311033a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/311033a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing