Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Antibodies against platelet-derived growth factor inhibit acute transformation by simian sarcoma virus

Nature volume 317, pages 438–440 (1985)Cite this article

Abstract

A clue to the molecular mechanism of neoplastic transformation was provided by the finding of a near identity in amino-acid sequence between the platelet-derived growth factor (PDGF) B-chain and a region in the transforming protein, p28sis, of simian sarcoma virus (SSV)1–4, an agent that causes sarcomas and gliomas in experimental animals5,6. This finding infers a direct link between the molecular biology of normal mitogenesis and oncogenesis since it suggests that the transforming activity of SSV is caused by a growth factor. Although PDGF agonist activity has been isolated from conditioned medium of SSV-transformed cells7–10, it is not clear whether infection of responsive cells by SSV leads solely to autocrine stimulation of growth by a secreted PDGF-like factor or whether other, possibly intracellular, activities of p28sis or its processed products contribute to the transformation9,11,12. To distinguish between these possibilities, we have studied the effect of anti-PDGF antibodies on acute SSV-transformation, and report here that these antibodies inhibit both proliferation and SSV-induced morphological changes in human diploid fibroblasts.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Waterfield, M. D. et al. Nature 304, 35–39 (1983).

    Article  ADS  CAS  Google Scholar 

  2. Doolittle, R. F. et al. Science 221, 275–277 (1983).

    Article  ADS  CAS  Google Scholar 

  3. Johnsson, A. et al. EMBO J. 3, 921–928 (1984).

    Article  CAS  Google Scholar 

  4. Devare, S. G., Reddy, E. P., Law, J. D., Robbins, K. C. & Aaronson, S. A. Proc. natn. Acad. Sci. U.S.A. 80, 731–735 (1983).

    Article  ADS  CAS  Google Scholar 

  5. Deinhardt, F. et al. J. med. Primatol. 1, 29–50 (1972).

    Article  CAS  Google Scholar 

  6. Johnson, L. Jr et al. Abstr. Proc. Am. Ass. Cancer Res. 16, 119 (1975).

    Google Scholar 

  7. Bowen-Pope, D. F., Vogel, A. & Ross, R. Proc. natn. Acad. Sci. U.S.A. 81, 2396–2400 (1984).

    Article  ADS  CAS  Google Scholar 

  8. Owen, A. J., Pantazis, P. & Antoniades, H. N. Science 225, 54–56 (1984).

    Article  ADS  CAS  Google Scholar 

  9. Huang, J. S., Huang, S. S. & Deuel, T. F. Cell 39, 79–87 (1984).

    Article  CAS  Google Scholar 

  10. Johnsson, A., Betsholtz, C., Von der Helm, K., Heldin, C.-H. & Westermark, B. Proc. natn. Acad. Sci. U.S.A. 82, 1721–1725 (1985).

    Article  ADS  CAS  Google Scholar 

  11. Robbins, K. C., Antoniades, H. N., Devare, S. G., Hunkapiller, M. W. & Aaronson, S. A. Nature 305, 605–608 (1983).

    Article  ADS  CAS  Google Scholar 

  12. Deuel, T. F., Huang, J. S., Huang, S. S., Stroobant, P. & Waterfield, M. D. Science 221, 1348–1350 (1983).

    Article  ADS  CAS  Google Scholar 

  13. Ponten, J. Virol. Monogr. 8, 25–27 (1971).

    Google Scholar 

  14. Stoker, M. G. P. Nature 246, 200–203 (1973).

    Article  ADS  CAS  Google Scholar 

  15. Heldin, C.-H., Westermark, B. & Wasteson, Å. Expl Cell Res. 136, 255–261 (1981).

    Article  CAS  Google Scholar 

  16. Betsholtz, C. & Westermark, B. J. Cell. Physiol. 118, 203–210 (1984).

    Article  CAS  Google Scholar 

  17. Johnsson, A., Heldin, C.-H., Westermark, B. & Wasteson, Å. Biochem. biophys. Res. Commun. 104, 66–74 (1982).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical and Physiological Chemistry, University of Uppsala, S-751 23, Uppsala, Sweden

    Ann Johnsson & Carl-Henrik Heldin

  2. Department of Pathology, University of Uppsala, S-751 23, Uppsala, Sweden

    Christer Betsholtz & Bengt Westermark

Authors
  1. Ann Johnsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christer Betsholtz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carl-Henrik Heldin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bengt Westermark
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Johnsson, A., Betsholtz, C., Heldin, CH. et al. Antibodies against platelet-derived growth factor inhibit acute transformation by simian sarcoma virus. Nature 317, 438–440 (1985). https://doi.org/10.1038/317438a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/317438a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing