Abstract
A rapid, transient rise in the free cytosolic Ca2+ concentration ([Ca2+]i) is one of the earliest events in neutrophil activation and is assumed to be involved in many of the subsequent cellular reactions1–3. Both Ca2+ release from intracellular stores and Ca2+ influx from the extracellular space contribute to the rise in [Ca2+]i4. In an attempt to assess the relative importance of these pools and the sequences leading to the rise in [Ca2+]i, we have studied the time course of changes in [Ca2+]i after stimulation with N-formylmethionyl-leucyl-phenylalanine (fMLP) or platelet-activating factor (PAF) using the Ca2+ indicators quin-2 and fura-2. We observed a time lag of 1–3 s between stimulation and rise in [Ca2+]i. This lag depends on the agonist concentration but is independent of extracellular Ca2+. Thus Ca2+ release from intracellular stores is rate limiting for the rise in [Ca2+]i. After this, cation channels in the plasma membrane (measured with the patch clamp method5) are opened. These non-selective channels, which also pass Ca2+, are activated by the initial rise in [Ca2+]i, but by neither fMLP nor inositol 1,4,5-trisphosphate (IP3) directly.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Pozzan, T., Lew, D. P., Wollheim, C. B. & Tsien, R. Y. Science 221, 1413–1415 (1983).
White, J. R., Naccache, P. H., Molski, T. F. P., Borgeat, P. & Sha'afi, R. I. Biochem. biophys. Res. Commun. 113, 44–50 (1983).
Gennaro, R., Pozzan, T. & Romeo, D. Proc. natn. Acad. Sci. U.S.A. 81, 1416–1420 (1984).
Korchak, H. M. et al. J. biol. Chem. 259, 4076–4082 (1984).
Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Pflügers Arch. ges. Physiol. 391, 85–100 (1981).
Tsien, R. Y. Biochemistry 19, 2396–2404 (1980).
Grynkiewicz, G., Poenie, M. & Tsien, R. Y. J. biol. Chem. 260, 3440–3450 (1985).
Sklar, L. A. et al. J. biol. Chem. 260, 11461–11467 (1985).
Sklar, L. A. & Oades, Z. G. J. biol. Chem. 260, 11468–11475 (1985).
von Tscharner, V., Derenleau, D. A. & Baggiolini, M. J. biol. Chem. 261, 10163–10168 (1986).
Di Virgilio, F., Lew, D. P. & Pozzan, T. Nature 310, 691–693 (1984).
Petersen, O. H. & Petersen, C. C. H. News physiol. Sci. 1, 5–8 (1986).
Spät, A., Bradford, P. G., McKinney, J. S., Rubin, R. P. & Putney, J. W. Jr Nature 319, 514–516 (1986).
Berridge, M. J. & Irvine, R. F. Nature 312, 315–321 (1984).
Korchak, H. M., Vienne, K., Rutherford, L. E. & Weissmann, G. Fedn Proc. 43, 2749–2754 (1984).
Fukushima, Y. & Hagiwara, S. J. Physiol., Lond. 358, 255–284 (1985).
Colquhoun, D., Neher, E., Reuter, H. & Stevens, C. F. Nature 294, 752–754 (1981).
Yellen, G. Nature 296, 357–359 (1982).
Barrowman, M. M., Cockcroft, S. & Gomperts, B. D. Nature 319, 504–507 (1986).
Sawyer, D. W., Sullivan, J. A. & Mandell, G. L. Science 230, 663–666 (1985).
Bøyum, A. Scand. J. clin. Invest. 21 (Suppl. 97), 77–79 (1986).
Hesketh, T. R., Smith, G. A., Moore, J. P., Taylor, M. V. & Metcalfe, J. C. J. biol. Chem. 25, 4876–4882 (1983).
Almers, W. & Neher, E. FEBS Lett. 192, 13–18 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
von Tscharner, V., Prod'hom, B., Baggiolini, M. et al. Ion channels in human neutrophils activated by a rise in free cytosolic calcium concentration. Nature 324, 369–372 (1986). https://doi.org/10.1038/324369a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/324369a0
This article is cited by
-
B cell zone reticular cell microenvironments shape CXCL13 gradient formation
Nature Communications (2020)
-
The function of TRP channels in neutrophil granulocytes
Pflügers Archiv - European Journal of Physiology (2018)
-
Signalling mechanisms in PAF-induced intestinal failure
Scientific Reports (2017)
-
On the activation mechanism of store-operated calcium channels
Pflügers Archiv - European Journal of Physiology (2006)
-
In vitro and ex vivo antioxidant activities of labetalol on rabbit neutrophil respiratory burst
Advances in Therapy (2004)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.