Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Three-dimensional structure of a complex of antibody with influenza virus neuraminidase

Abstract

The structure of a complex between influenza virus neuraminidase and an antibody displays features inconsistent with the inflexible 'lock and key' model of antigen‐antibody binding. The structure of the antigen changes on binding, and that of the antibody may also change; the interaction therefore has some of the character of a handshake.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Colman, P. M. & Ward, C. W. Curr. Top. Microbiol. Immun. 114, 178–255 (1985).

    Google Scholar 

  2. Kabat, E. A., Wu, T. T., Bilofsky, H., Reid-Miller, M. & Perry, H. Sequences of Proteins of Immunological Interest (US Department of Health and Public Services, Washington DC, 1983).

    Google Scholar 

  3. Schiffer, M., Girling, R. L., Ely, K. R. & Edmundson, A. B. Biochemistry 12, 4620–4631 (1973).

    Article  CAS  PubMed  Google Scholar 

  4. Poljak, R. J. et al. Proc. natn. Acad. Sci. U.S.A. 70, 3305–3310 (1973).

    Article  ADS  CAS  Google Scholar 

  5. Segal, D. M. et al. Proc. natn. Acad. Sci. U.S.A. 71, 4298–4302 (1974).

    Article  ADS  CAS  Google Scholar 

  6. Matsushima, M. et al. J. molec. Biol. 121, 441–459 (1978).

    Article  CAS  PubMed  Google Scholar 

  7. Saul, F., Amzel, L. M. & Poljak, R. J. J. biol. Chem. 253, 585–597 (1978).

    CAS  PubMed  Google Scholar 

  8. Marquart, M., Deisenhofer, J., Huber, R. & Palm, W. J. molec. Biol. 141, 369–392 (1980).

    Article  CAS  PubMed  Google Scholar 

  9. Satow, Y., Cohen, O. H., Padlan, E. A. & Davies, D. R. J. molec. Biol. 190, 593–604 (1986).

    Article  CAS  PubMed  Google Scholar 

  10. Suh, S. W. et al. Proteins: Structure, Function and Genetics, 1, 74–80 (1986).

    Article  CAS  Google Scholar 

  11. Colman, P. M. & Webster, R. G. in Biological Organisation: Macromolecular Interactions at High Resolution (ed. Burnett, R.) 125–133 (Academic, New York, 1987).

    Google Scholar 

  12. Amit, A. G., Marriuzza, R. A., Phillips, S. E. V. & Poljak, R. J. Science 233, 747–753 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

  13. Novotny, J. & Haber, E. Proc. natn. Acad. Sci. U.S.A. 82, 4592–4596 (1985).

    Article  ADS  CAS  Google Scholar 

  14. Davies, D. R., Padlan, E. A. & Segal, D. M. A. Rev. Biochem. 44, 639–667 (1975).

    Article  CAS  Google Scholar 

  15. Chothia, C., Novotny, J., Bruccoleri, R. & Karplus, M. J. molec. Biol. 186, 651–663 (1986).

    Article  Google Scholar 

  16. Padlan, E. A., Cohen, G. H. & Davies, D. R. Molec. Immun. 23, 951–960 (1986).

    Article  CAS  PubMed  Google Scholar 

  17. Colman, P. M., Deisenhofer, J., Huber, R. & Palm, W. J. molec. Biol. 100, 257–282 (1976).

    Article  CAS  PubMed  Google Scholar 

  18. Davies, D. R. & Metzger, H. A. Rev. Immun. 1, 87–117 (1983).

    Article  CAS  Google Scholar 

  19. Benjamin, D. C. et al. A. Rev. Immun. 2, 67–101 (1984).

    Article  CAS  Google Scholar 

  20. Crumpton, M. J. & Wilkinson, J. M. Biochem. J. 94, 545–556 (1965).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Smith-Gill, S. J. et al. J. Immun. 128, 314–322 (1982).

    CAS  PubMed  Google Scholar 

  22. Lerner, R. A. Nature 299, 592–596 (1982).

    Article  ADS  CAS  Google Scholar 

  23. Tainer, J. A. et al. Nature 312, 127–134 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  24. Westhof, E. et al. Nature 311, 123–126 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  25. Metzger, H. Contemp. Top. molec. Immun. 7, 119–152 (1978).

    Article  CAS  Google Scholar 

  26. Laver, W. G. Virology 86, 78–87 (1978).

    Article  CAS  PubMed  Google Scholar 

  27. Schulman, J. L. in The Influenza Viruses and Influenza (ed. Kilbourne, E. D.) 373–393 (Academic, New York, 1975).

    Google Scholar 

  28. Webster, R. G., Laver, W. G. & Air, G. M. in Genetics of Influenza Viruses (ed. Palese, P. & Kingsbury, B. W.) 127–168 (Springer, New York, 1983).

    Book  Google Scholar 

  29. Varghese, J. N., Laver, W. G. & Colman, P. M. Nature 303, 35–40 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  30. Colman, P. M., Varghese, J. N. & Laver, W. G. Nature 303, 41–44 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  31. Laver, W. G., Colman, P. M., Webster, R. G., Hinshaw, V. S. & Air, G. M. Virology 137, 314–323 (1984).

    Article  CAS  PubMed  Google Scholar 

  32. Air, G. M., Ritchie, L. R., Laver, W. G. & Colman, P. M. Virology 145, 117–122 (1985).

    Article  CAS  PubMed  Google Scholar 

  33. Tulloch, P. A. et al. J. molec. Biol. 190, 215–225 (1986).

    Article  CAS  PubMed  Google Scholar 

  34. Laver, W. G., Webster, R. G. & Colman, P. M. Virology 156, 181–184 (1987).

    Article  CAS  PubMed  Google Scholar 

  35. Epp, O. et al. Biochemistry 14, 4943–4952 (1975).

    Article  CAS  PubMed  Google Scholar 

  36. Epp, O. et al. Eur. J. Biochem. 45, 513–524 (1974).

    Article  CAS  PubMed  Google Scholar 

  37. Colman, P. M., Schramm, H. J. & Guss, J. M. J. molec. Biol. 116, 73–79 (1977).

    Article  CAS  PubMed  Google Scholar 

  38. Furey, W., Wang, B. C., Yoo, C. S. & Sax, M. J. molec. Biol. 167, 661–692 (1983).

    Article  CAS  PubMed  Google Scholar 

  39. Chang, C. H. et al. Biochemistry 24, 4890–4897 (1985).

    Article  CAS  PubMed  Google Scholar 

  40. Holowka, D. A., Strosberg, A. D., Kimball, J. W., Haber, E. & Cathou, R. E. Proc. natn. Acad. Sci. U.S.A. 69, 3399–3403 (1972).

    Article  ADS  CAS  Google Scholar 

  41. Levison, S. A., Hicks, A. N., Portman, A. J. & Dandliker, W. B. Biochemistry 14, 3778–3786 (1975).

    Article  CAS  PubMed  Google Scholar 

  42. Schlessinger, J., Steinberg, I. Z., Givol, I. D., Hochman, J. & Pecht, I. Proc. natn. Acad. Sci. U.S.A. 72, 2775–2779 (1975).

    Article  ADS  CAS  Google Scholar 

  43. Lancet, D. & Pecht, I. Proc. natn. Acad. Sci. U.S.A. 73, 3549–3553 (1976).

    Article  ADS  CAS  Google Scholar 

  44. Zidovetzki, R., Blatt, Y. & Pecht, I. Biochemistry 20, 5011–5018 (1981).

    Article  CAS  PubMed  Google Scholar 

  45. Edmundson, A. B., Ely, K. R. & Herron, J. N. Molec. Immun. 21, 561–576 (1984).

    Article  CAS  PubMed  Google Scholar 

  46. Ely, K. R. et al. Biochemistry 17, 158–167 (1978).

    Article  CAS  PubMed  Google Scholar 

  47. Jackson, D. C. & Webster, R. G. Virology 123, 69–77 (1982).

    Article  CAS  PubMed  Google Scholar 

  48. Mandel, B., Virology 69, 500–510 (1976).

    Article  CAS  PubMed  Google Scholar 

  49. Lubeck, M. & Gerhard, W. Virology 118, 1–7 (1982).

    Article  CAS  PubMed  Google Scholar 

  50. Crumpton, M. J. Biochem. J. 100, 223–232 (1966).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  51. Amon, R. The Antigens Vol. 1 (ed. Sela, M.) 87–159 (Academic, New York, 1973).

    Google Scholar 

  52. Dimmock, N. J. J. gen. Virol 65, 1015–1022 (1984).

    Article  PubMed  Google Scholar 

  53. Srinivasappa, J. et al. J. Virol. 57, 397–401 (1986).

    CAS  PubMed  PubMed Central  Google Scholar 

  54. Tonegawa, S. Sci. Am. 253, 104–113 (1985).

    Article  Google Scholar 

  55. Barth, R. K. et al. Nature 316, 517–523 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  56. Arden, B., Klotz, J., Siu, G. & Hood, L. E. Nature 316, 783–787 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  57. Goverman, J., Hunkapillar, T. & Hood, L. E. Cell 45, 475–484 (1986).

    Article  CAS  PubMed  Google Scholar 

  58. Amyard-Henry, M. et al. Bull. WHO 48, 199–202 (1973).

    Google Scholar 

  59. Air, G. M. Virology 97, 468–472 (1979).

    Article  CAS  PubMed  Google Scholar 

  60. Wang, B. C. Meth. Enzym. 115, 90–112 (1985).

    Article  CAS  PubMed  Google Scholar 

  61. Sussman, J. L. in Methods and Applications in Crystallographic Computing (eds Hall, S. R. & Ashida, T.) 206–237 (Oxford University Press, 1984).

    Google Scholar 

  62. Hendrickson, W. A. & Konnert, J. H. in Biomolecular Structure, Conformation, Function and Evolution Vol. 1 (ed. Srinivasan, R.) 43–57 (Pergamon, Oxford, 1981).

    Book  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Colman, P., Laver, W., Varghese, J. et al. Three-dimensional structure of a complex of antibody with influenza virus neuraminidase. Nature 326, 358–363 (1987). https://doi.org/10.1038/326358a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/326358a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing