Abstract
The external region of a cell membrane, known as the glycocalyx, is dominated by glycosylated molecules1,2,3 which direct specific interactions such as cell–cell recognition and contribute to the steric repulsion that prevents undesirable non-specific adhesion of other molecules and cells. Mimicking the non-adhesive properties of a glycocalyx provides a potential solution to the clinical problems, such as thrombosis4, that are associated with implantable devices owing to non-specific adsorption of plasma proteins. Here we describe a biomimetic surface modification of graphite using oligosaccharide surfactant polymers, which, like a glycocalyx, provides a dense and confluent layer of oligosaccharides. The surfactant polymers consist of a flexible poly(vinyl amine) with dextran and alkanoyl side chains. We show that alkanoyl side chains assemble on graphite through hydrophobic interaction and epitaxial adsorption. This constrains the polymer backbone to lie parallel to the substrate, with solvated dextran side chains protruding into the aqueous phase, creating a glycocalyx-like coating. The resulting biomimetic surface is effective in suppressing protein adsorption from human plasma protein solution.
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Acknowledgements
We acknowledge the financial support for this work provided by the National Institutes of Health and the Whitaker Foundation, and use of the AFM and IR facilities at the Center for Cardiovascular Biomaterials, CWRU.
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Holland, N., Qiu, Y., Ruegsegger, M. et al. Biomimetic engineering of non-adhesive glycocalyx-like surfaces using oligosaccharide surfactant polymers. Nature 392, 799–801 (1998). https://doi.org/10.1038/33894
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DOI: https://doi.org/10.1038/33894
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