Abstract
TRUE Upases attack triacylglycerols and act at an oil–water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 Å resolution and refined to 1.9 Å resolution. It reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
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References
Huge-Jensen, B., Gailuzzo Rubano, D. & Jensen, R. G. Lipids 22, 559–565 (1987).
Boel, E., Huge-Jensen, B., Christensen, M., Thim, L. & Fiil, N. P. Lipids 23, 701–706 (1988).
Huge-Jensen, B. et al. Lipids 24, 781–785 (1989).
Matthews, B. W. J. molec. Biol. 33, 491–497 (1968).
Hendrickson, W. A. Meth. Enzym. 115, 252–270 (1985).
Brunger, A. T., Karplus, M. & Petsko, G. A. Acta crystallogr. A45, 50–61 (1989).
Jones, T. A. J. appl. Crystallogr. 11, 268–272 (1978).
Rees, D. C., Lewis, M. & Lipscomb, W. N. J. molec. Biol. 168, 367–387 (1983).
Salemme, F. R. Prog. Biophys. molec. Biol. 42, 95–133 (1983).
Kugimiya, W., Otani, Y., Hashimoto, Y. & Takagi, Y. Biochem. biophys. Res. Commun. 141, 185–190 (1986).
Lee, C. W. & landolo, J. J. J. Bact. 166, 385–391 (1984).
Gotz, F., Popp, F., Korn, E. & Schleifer, K. H. Nucleic Acids Res. 13, 5895–5906 (1985).
Docherty, A. J. P. et al. Nucleic Acids Res. 13, 1891–1903 (1985).
Kerfelec, B., LaForge, K. S., Puigserver, A. & Scheele, G. Pancreas 1, 430–437 (1986).
Wion, K. L., Kirchgessner, T. G., Lusis, A. J., Schotz, M. C. & Lawn, R. M. Science 235, 1638–1641 (1987).
Senda, M., Oka, K., Brown, W. V., Qasba, P. K. & Furuichi, Y. Proc. natn. Acad. Sci. U.S.A. 84, 4369–4373 (1987).
Kirchgessner, T. G., Svenson, K. L., Lusis, A. J. & Schotz, M. C. J. biol. Chem. 262, 8463–8466 (1987).
Bernstein, F. C. et al. J. molec. Biol. 112, 535–542 (1977).
Derewenda, Z. & Helliwell, J. R. J. appl. Crystallogr. 22, 123–137 (1989).
Read, R. J. & James, M. N. J. J. molec. Biol. 200, 523–551 (1988).
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Brady, L., Brzozowski, A., Derewenda, Z. et al. A serine protease triad forms the catalytic centre of a triacylglycerol lipase. Nature 343, 767–770 (1990). https://doi.org/10.1038/343767a0
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DOI: https://doi.org/10.1038/343767a0
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