Use of Escherichia coli as an expression host has opened up new possibilities in antibody research and its applications. It greatly facilitates rational engineering and random mutagenesis.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Skerra, A. & Plückthun, A. Science 240, 1038–1041 (1988).
Plückthun, A. & Skerra, A. Meth. Enzym. 178, 497–515 (1989).
Better, M., Chang, C.P., Robinson, R.R. & Horwitz, A.H. Science 240, 1041–1043 (1988).
Glockshuber, R., Malia, M., Pfitzinger, I. & Plückthun, A. Biochemistry 29, 1362–1367 (1990).
Skerra, A. & Plückthun, A. (submitted).
Perlmutter, R.M. et al. Adv. Immun. 35, 1–37 (1984).
Plückthun, A., Glockshuber, R., Pfitzinger, I., Skerra, A. & Stadlmüller, J. Cold Spring Harb. Symp. quant. Biol. 52, 105–112 (1987).
Segal, D.M., Padlan, E.A., Cohen, G.H., Rudikoff, S., Potter, M. & Davies, D.R. Proc. Natn Acad. Sci. U.S.A. 71, 4298–4302 (1974).
Satow, Y., Cohen, G.H., Padlan, E.A. & Davies, D.R. J. molec. Biol. 190, 593–604 (1986).
Skerra, A., Glockshuber, A. & Plückthun, A. (submitted).
Bird, R.E. et al. Science 242, 423–426 (1988).
Huston, J.S. et al. Proc. Natn Acad. Sci. U.S.A. 85, 5879–5883 (1988).
Ward, E.S., Güssow, D., Griffiths, A.D., Jones, P.T. & Winter, G. Nature 341, 544–546 (1989).
Glockshuber, R., Steipe, B., Huber, R. & Plückthun, A. J. molec. Biol. 213, 613–615 (1990).
Huse, W.D. et al. Science 246, 1275–1281 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Plückthun, A. Antibodies from Escherichia coli. Nature 347, 497–498 (1990). https://doi.org/10.1038/347497a0
Issue Date:
DOI: https://doi.org/10.1038/347497a0
This article is cited by
-
Solution properties ofEscherichia coli-expressed VH domain of anti-neuraminidase antibody NC41
Journal of Protein Chemistry (1995)
-
Recombinant Colorimetric Antibodies: Construction and Characterization of a Bifunctional F(ab)2/Alkaline Phosphatase Conjugate Produced in Escherichia coli
Nature Biotechnology (1993)
-
Efficient Production of a Functional Single-Chain Antidigoxin Antibody via an Engineered Bacillus subtilis Expression-Secretion System
Nature Biotechnology (1993)
-
Antibody Engineering: Advances From the Use of Escherichia coli Expression Systems
Nature Biotechnology (1991)