Abstract
I N synapses, a rise in presynaptic intracellular calcium leads to secretory vesicle fusion in less than a millisecond, as indicated by the short delay from excitation to postsynaptic signal1–4. In non-synaptic secretory cells, studies at high time resolution have been limited by the lack of a detector as fast and sensitive as the postsynaptic membrane. Electrochemical methods may be sensitive enough to detect catecholamines released from single vesicles5,6. Here, we show that under voltage-clamp conditions, stochastically occurring signals can be recorded from adrenal chromaffin cells using a carbon-fibre electrode as an electrochemical detector. These signals obey statistics characteristic for quantal release; however, in contrast to neuronal transmitter release, secretion occurs with a significant delay after short step depolarizations. Furthermore, we identify a pedestal or 'foot' at the onset of unitary events which may represent the slow leak of catecholamine molecules out of a narrow 'fusion pore' before the pore dilates for complete exocytosis.
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References
Del Castillo, J. & Katz, B. J. Physiol., Lond. 124, 560–573 (1954).
Llinas, R., Steinberg, I. Z. & Walton, K. Biophys. J. 33, 323–352 (1981).
Augustine, G. J., Charlton, M. P. & Smith, S. J. J. Physiol., Lond. 367, 163–181 (1985).
Edwards, F. A., Konnerth, A. & Sakmann, B. J. Physiol., Lond. 430, 213–249 (1990).
Leszczyszyn, D. J. et al. J. biol. Chem. 265, 14736–14737 (1990).
Leszczyszyn, D. J. et al. J. Neurochem. 56, 1855–1863 (1991).
Fenwick, E. M., Marty, A. & Neher, E. J. Physiol., Lond. 331, 599–635 (1982).
Neher, E. & Marty, A. Proc. natn. Acad. Sci. U.S.A. 79, 6712–6716 (1982).
Duchen, M. R., Millar, J. & Biscoe, T. J. J. Physiol., Lond. 426, 5 (1991).
Marsden, C. A. et al. Neuroscience 25, 389–400 (1988).
Lindau, M. & Neher, E. Pflügers Arch. 411, 137–146 (1988).
Baur, J. E., Kristensen, E. W., May, L. J., Wiedemann, D. J. & Wightman, R. W. Analyt. Chem. 60, 1268–1272 (1988).
Phillips, J. H. Neuroscience 7, 1595–1609 (1982).
Katz, B. & Miledi, R. J. Physiol., Lond. 181, 656–670 (1965).
Horn, R. & Marty, A. J. gen. Physiol. 92, 145–159 (1988).
Aimers, W. A. Rev. Physiol. 52, 607–624 (1990).
Thomas, L. et al. Science 242, 1050–1053 (1988).
Parnas, H., Dudel, J. & Parnas, I. Pflügers Arch. 406, 121–130 (1986).
Simon, S. M. & Llinas, R. Biophys. J. 48, 485–498 (1985).
Fogelson, A. L. & Zucker, R. S. Biophys. J. 48, 1003–1017 (1985).
Sala, F. & Hernandez-Cruz, A. Biophys. J. 57, 313–324 (1990).
Alvarez De Toledo, G. & Fernandez, J. M. J. gen. Physiol. 95, 397–409 (1990).
Marty, A. & Neher, E. J. Physiol., Lond. 367, 117–141 (1985).
Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Pflügers Arch. 391, 85–100 (1981).
Armstrong-James, M. & Millar, J. J. Neurosci. Meth. 1, 279–287 (1979).
Crank, J. The Mathematics of Diffusion 2nd edn (Oxford University Press, Oxford, 1975).
Jackson, J. D. Classical Electrodynamics 2nd edn (Wiley, New York, 1975).
Wightman, R. M. et al. Proc. natn. Acad. Sci. U.S.A. 88, 10754–10758 (1991).
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Chow, R., von Rüden, L. & Neher, E. Delay in vesicle fusion revealed by electrochemical monitoring of single secretory events in adrenal chromaffin cells. Nature 356, 60–63 (1992). https://doi.org/10.1038/356060a0
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DOI: https://doi.org/10.1038/356060a0
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