A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences. Quantitative detection of such mixtures of HIV-1 drug resistance mutations was best achieved using an automated system that uses fluorescent-labelled sequencing primers. This procedure has a wide range of applications in clinical research, including heterozygote analysis. Software that automatically reports mixed-base positions is presented.
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Larder, B., Kohli, A., Kellam, P. et al. Quantitative detection of HIV-1 drug resistance mutations by automated DNA sequencing. Nature 365, 671–673 (1993). https://doi.org/10.1038/365671a0
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DOI: https://doi.org/10.1038/365671a0
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