Abstract
SMAD proteins1 have been identified as signalling mediators of the TGF-β superfamily, which is involved in a range of biological activities including cell growth, morphogenesis, development and immune responses2,3. Smad1, Smad2, Smad3 and Smad5 are ligand-specific: Smad1 and Smad5 transduce signals from bone morphogenetic proteins4,5,6,7, and Smad2 and Smad3 mediate signalling by TGF-β and activin8,9, whereas Smad4 acts as a common signalling component10. For example, Smad2 is phosphorylated by the TGF-β type I receptor upon ligand binding, forms a heteromer with Smad4, and then translocates into the nucleus where it activates transcription10,11. Here we report the isolation of Smad6 in the mouse. Smad6 is quite different in structure from the other SMAD proteins, and forms stable associations with type I receptors. Smad6 interferes with the phosphorylation of Smad2 and the subsequent heteromerization with Smad4, but does not inhibit the activity of Smad3. Smad6 also inhibits the phosphorylation of Smad1 that is induced by the bone morphogenetic protein type IB receptor. These data indicate that signals of the TGF-β superfamily are regulated both positively and negatively by members of the SMAD family.
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Acknowledgements
We thank A. Nakao for the Smad1 and Smad2 cDNAs; S. Kern for the Smad4/DPC4 cDNA; J. Massagué for the p3TP-Lux reporter and R mutant cells; R. Derynck for Smad3 cDNA and the pCAL2 cyclin A reporter; Y. Eto for activin A; T. K. Sampath for OP-1/BMP-7; P. Horton for critical reading of the manuscript; and Y. Inada for technical assistance. This work was supported by grants-in-aid for scientific research from the Ministry of Education, Science, Sports and Culture of Japan, and special coordiantion funds for promoting science and technology from the Science and Technology Agency. K.M. is supported by the Toray Scientific Foundation and Uehara Memorial Foundation.
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Imamura, T., Takase, M., Nishihara, A. et al. Smad6 inhibits signalling by the TGF-β superfamily. Nature 389, 622–626 (1997). https://doi.org/10.1038/39355
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DOI: https://doi.org/10.1038/39355
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