Conjugation of small ubiquitin-related modifier (SUMO) to lysine residues in target proteins is a multistep enzymatic reaction analogous to ubiquitination.1 Protein SUMOylation regulates numerous biological processes including transcription, the cell cycle, DNA repair and innate immunity.1 In the first step of the reaction, SUMO is cleaved from the SUMO precursor by SUMO-specific proteases. Next, SUMO is bound to the cysteine residue of the SUMO-activating enzyme (E1), forming a thioester linkage in an ATP-dependent manner. SUMO is then transferred from E1 to the cysteine residue of the SUMO-conjugating enzyme (E2). Finally, SUMO ligase (E3) catalyzes the SUMOylation of specific substrates via a direct interaction with E2 and the substrates. Like ubiquitination, SUMOylation is reversible; the deSUMOylation process is mediated by SUMO-specific proteases. Abnormal SUMOylation is implicated in various diseases including neurodegenerative disease,2 viral infection3 and cancer.4, 5 Therefore, enzymes responsible for the SUMO conjugation pathway represent potential targets for drug discovery.
To date, several natural products including ginkgolic acid,6 anacardic acid,6 kerriamycin B7 and spectomycin B18 as well as synthetic compounds,9 have been reported to inhibit protein SUMOylation. Here, we report another natural product that functions as a SUMOylation inhibitor: davidiin, purified from the plant Davidia involucrata. Although most known SUMOylation inhibitors function in the micromolar range, davidiin is particularly potent, inhibiting at sub-micromolar concentrations.
Materials for this study were obtained as follows. Goat polyclonal anti-SUMO-1 (N-19) and goat polyclonal anti-p53 (FL393)-G antibodies were purchased from Santa Cruz Biotechnologies (Santa Cruz, CA, USA). A mouse monoclonal anti-T7 antibody was from Novagen (Darmstadt, Germany). Mouse monoclonal anti-α-tubulin (B-5-1-2) and anti-FLAG (M2) antibodies were purchased from Sigma (St. Louis, MO, USA). Recombinant His- and T7-tagged RanGAP1-C2, GST-Aos1-Uba2 fusion protein (E1), His-tagged Ubc9 (E2), and His-tagged SUMO-1 proteins were purified as described previously.10 293T, H1299, MKN-45, DU-145 and NCI-H460 cells were maintained in Dulbecco’s modified Eagle medium supplemented with 10% FBS at 37 °C under 5% CO2.
The in vitro SUMOylation reaction was performed as described.6 Briefly, in vitro SUMOylation reaction was performed for 2 h at 30 °C in 20 μl buffer (50 mM Tris-HCl (pH 7.4), 6 mM MgCl2, 2 mM ATP and 1 mM dithiothreitol) containing His- and T7-tagged RanGAP1-C2, GST-Aos1/Uba2 (E1), His-tagged Ubc9 and His-tagged SUMO-1. Samples were separated by 10% SDS–PAGE followed by immunoblotting using an anti-T7 antibody and an anti-SUMO-1 antibody.
The reaction for thioester bond formation between SUMO and E1 was performed as described.6 Briefly, the reaction for the thioester bond formation was performed for 20 min at 37 °C in 20 μl buffer (50 mM Tris-HCl (pH 7.4), 6 mM MgCl2, 2 mM ATP) containing GST-Aos1/Uba2 (E1) and biotinylated SUMO-1 in the absence of dithiothreitol. Samples were separated by 11% SDS–PAGE and the E1–biotinylated SUMO-1 intermediate was detected by avidin-conjugated horseradish peroxidase (Sigma).
A screen of 750 samples of botanical and food ingredients extracts using an in situ cell-based SUMOylation assay11 revealed several samples that could inhibit protein SUMOylation, including an extract of D. involucrata (data not shown).6 The inhibitory activity of the D. involucrata extract was confirmed by in vitro SUMOylation assay using RanGAP1-C2 as substrate (Figure 1a). Compound A was isolated by activity-guided fractionation and it was identified by mass spectrometry (MS) (JMS-700, JEOL Ltd, Tokyo, Japan), NMR (EX-400, JEOL Ltd), UV (UV mini 1240, Shimadzu Co., Kyoto, Japan), and optical rotation (DIP1000, JASCO Co., Tokyo, Japan). Briefly, leaves of D. involucrata (328.3 g) were harvested from the Botanical Gardens of Iwate University and dried naturally at room temperature. The dried leaves (113.0 g) were extracted with MeOH, and the extract was diluted with water. The MeOH extract (34.05 g) was extracted with ethyl acetate (1 vol, two times) and evaporated (8.96 g). Half of the ethyl acetate extract was subjected to a Diaion HP-20 column (3 cm diameter × 20 cm) and an active fraction was eluted with 50% MeOH two times repeatedly (1.06 g). Biologically active peak A was isolated from part of the 50% MeOH fraction (100.0 mg) using an HPLC system (880-PU (pump) equipped with an MD-910 photodiode array detector (JASCO, Tokyo, Japan)) on a Capcell Pak ODS (20 mm diameter × 250 mm; Shiseido Inc., Tokyo, Japan) with 15% CH3CN–0.1% CH3COOH at a flow rate of 5 ml min−1. After the peak A fraction was isolated, it was subjected to HP-20 column (2.1 cm diameter × 7.0 cm) and eluted with 50% MeOH. The product was obtained as a light brownish powder after lyophilization (18.0 mg).
Data obtained were as follows: HR-FAB-MS m/z (M+H)+, calculated for C41H31O26, 939.1104; found, 939.1152; 1H NMR δH of the glucose moiety of compound A (400 MHz, acetone-d6) 6.13 (1H, d, J=2.7), 5.53 (1H, dd, J=2.7, 7.0), 5.79 (1H, dd, J=6.5, 7.0), 5.21 (1H, dd, J=2.7, 6.5), 4.57 (1H, ddd, J=2.7, 5.1, 12.1), 4.84 (1H, dd, J=11.7, 12.1), 4.42 (1H, dd, J=5.1, 11.7), 13C NMR δC (100 MHz, acetone-d6) 94,1 (C-1), 70.2 (C-2), 68.7 (C-3), 70.7 (C-4), 74.9 (C-5), 64.8 (C-6); the UV spectrum (nm) (ɛ) in MeOH 279 (49800); [α]D25=+20.2° (c=0.2, MeOH). All physicochemical properties were identical with data reported for a known ellagitannin, davidiin, previously isolated from D. involucrata (Figure 1b).12, 13, 14 Purified davidiin inhibited in vitro SUMOylation of RanGAP1-C2 in a dose-dependent manner, and its IC50 value was 0.15 μM (Figure 1c). We next examined whether davidiin affects in vivo protein SUMOylation by analyzing the levels of SUMOylated proteins in 293T cells expressing FLAG-tagged SUMO-1. Immunoblotting using an anti-FLAG antibody revealed that davidiin reduced the amount of high-molecular weight SUMO conjugates in a dose-dependent manner (Figure 1d). In addition, davidiin reduced the level of SUMOylation of p53 in a dose-dependent manner (Figure 1e). Importantly, davidiin did not influence the cellular level of ubiquitinated proteins (Figure 1f). These results indicate that davidiin inhibits protein SUMOylation both in vitro and in vivo without affecting protein ubiquitination. Next, we sought to identify the target of davidiin. The formation of an E1 conjugate to biotinylated SUMO-1 via a thioester linkage could be detected in the presence of ATP under non-reducing conditions; the band corresponding to the E1–biotinylated SUMO-1 intermediate disappeared when the reducing agent dithiothreitol or the SUMO E1 inhibitor ginkgolic acid6 was added to the reaction mixture (Figure 1g). The formation of an intermediate between E1 and biotinylated SUMO-1 was also blocked by davidiin in a dose range similar to that required for inhibition of in vitro SUMOylation (Figures 1c and g). These results suggest that davidiin inhibits protein SUMOylation by inhibiting the formation of the E1–SUMO-1 intermediate.
High-level expression of genes involved in SUMOylation was frequently observed in cancer. In addition, SUMO E1 has an important role in Myc-driven tumorigenesis.15 Accordingly, we tested effects of davidiin on proliferation of cancer cells. As shown in Figure 2, davidiin inhibited cell proliferation of several cancer cells in a dose-dependent manner with similar GI50 values (gastric cancer MKN-45 cells: 8.3 μM, prostate cancer DU-145 cells: 11.6 μM, lung cancer NCI-H460 cells: 16.4 μM).
Davidiin is an ellagitannin previously shown to inhibit the binding of a ligand to a μ-opioid receptor.16 In this study, we demonstrated a novel physiological activity of davidiin, namely, inhibition of protein SUMOylation. The IC50 value of davidiin against in vitro protein SUMOylation is 0.15 μM (Figure 1c), the most potent among small-molecule SUMOylation inhibitors reported to date.6, 7, 8, 9 Recent studies have shown that davidiin inhibits growth of hepatocellular carcinoma cells by downregulating EZH2,17 the enzymatic subunit of the Polycomb-repressive complex 2 that catalyzes histone H3 lysine 27 methylation, which is highly expressed in a variety of human cancers. Importantly in this regard, EZH2 is SUMOylated in cells.18 In this study, we showed that davidiin inhibited cell growth of several cancer cells including DU-145 cells, in which EZH2 is overexpressed (Figure 2).19 Importantly, concentrations of GI50 values of davidiin for these cancer cells were sufficient to remarkably inhibit protein SUMOylation in cells (Figures 1d and e). Although the molecular mechanism by which davidiin inhibits cancer cell growth remains to be elucidated, these observations suggested that the anti-tumor activity of davidiin is mediated, at least in part, by inhibition of SUMOylation of proteins including EZH2. The total synthesis of davidiin was recently established by Kasai et al.20 Because davidiin is the strongest natural SUMOylation inhibitor reported so far, it is an excellent starting material for drug discovery. Analyses of the structure–activity relationship of this compound should facilitate development of novel anticancer agents targeting protein SUMOylation.
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Acknowledgements
This work was supported in part by the Chemical Genomics Research Project; RIKEN Center for Sustainable Resource Science; the CREST Research Project; the Japan Science and Technology Corporation; Grants-in-Aid for Scientific Research (C); and Grants-in-Aid for Scientific Research on Innovative Area ‘Cancer’ from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
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Takemoto, M., Kawamura, Y., Hirohama, M. et al. Inhibition of protein SUMOylation by davidiin, an ellagitannin from Davidia involucrata. J Antibiot 67, 335–338 (2014). https://doi.org/10.1038/ja.2013.142
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DOI: https://doi.org/10.1038/ja.2013.142
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