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Pathway engineering for the production of aromatic compounds in Escherichia coli

Nature Biotechnology volume 14, pages 620–623 (1996)Cite this article

Abstract

Glucose is the preferred substrate for certain fermentation processes. During its internalization and concomitant formation of glucose-6-phosphate through the glucose phosphotransferase system (PTS), one molecule of phosphoenolpyruvate (PEP) is consumed. Together with erythrose 4-phosphate (E4P), PEP is condensed to form 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAMP), the first intermediate of the common segment of the aromatic pathway. From this metabolic route, several commercially important aromatic compounds can be obtained. We have selected Escherichia coli mutants that can transport glucose efficiently by a non-PTS uptake system. In theory, this process should increase the availability of PEP for other biosynthetic reactions. Using these mutants, in a background where the DAMP synthase (the enzyme that catalyzes the condensation of PEP and E4P into DAMP) was amplified, we were able to show that at least some of the PEP saved during glucose transport, can be redirected into the aromatic pathway. This increased carbon commitment to the aromatic pathway was enhanced still further upon amplification of the E. coli tktA gene that encodes for a transketolase involved in the biosynthesis of E4P.

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Author notes

  1. Fernando Valle: e-mail: valle@ibt.unam.mx.

Authors and Affiliations

  1. Institute de Biotecnología, Universidad National Autónoma de México, Apdo. Postal 510–3, Cuernavaca, Morelos, 62250, México

    Noemí Floras, Francisco Bolivar & Fernando Valle

  2. Genencor International Inc., 180 Kimball Way, South San Francisco, CA, 94080

    Jimmy Xiao & Alan Berry

Authors
  1. Noemí Floras
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  2. Jimmy Xiao
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  3. Alan Berry
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  4. Francisco Bolivar
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  5. Fernando Valle
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Floras, N., Xiao, J., Berry, A. et al. Pathway engineering for the production of aromatic compounds in Escherichia coli. Nat Biotechnol 14, 620–623 (1996). https://doi.org/10.1038/nbt0596-620

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