Abstract
Precursors singly and doubly labeled with stable isotopes can be used to study both the origins of antibiotic molecules and the mechanisms involved in their biosyntheses. In this review, the versatile methodology used for these approaches is discussed, using examples from the biosynthetic investigations of neomycin, ribostamycin, nybomycin and erythromycin and of two antibiotics containing the so–called m–C7N unit, pactamycin and gel–danamycin.
*The E. R. Squibb Lectures on Chemistry of Microbial Products presented at Waksman Institute of Microbiology, Rutgers University, New Brunswick, N.J. March 16, 17, 1983.
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Rinehart, K. Newer Methods for Characterization of Antibiotics I: Stable Isotopes in the Study of Antibiotic Synthesis. Nat Biotechnol 1, 495–502 (1983). https://doi.org/10.1038/nbt0883-495
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DOI: https://doi.org/10.1038/nbt0883-495
