Abstract
THE glycine-specific tRNAs of E. coli can be grouped into three subspecies which are separated by chromatography on benzoylated DEAE cellulose (BDC): tRNAGly1 (GGG), tRNAGly2 (GGA/G), and tRNAGly3 (GGU/C)1,2. The tRNAGly1 and tRNAGly2 are specified by the genes, glyU and glyT, respectively, which have been located at 55 and 77 minutes on the E. coli chromosome. Suppressors of tryptophan A gene (trpA) missense mutations and partial diploid strains have been used extensively to characterize the glycine tRNA structural genes (Table 1)1–3. A common property of these suppressor mutations is that the altered tRNAGly is no longer aminoacylated at the normal rate by the glycyl tRNA synthetase (GRS). When ordinary loading conditions are used virtually none of the suppressor tRNA species are amino-acylated. These studies have shown that single gene copies are normally present at the glyT and glyU loci.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
We are sorry, but there is no personal subscription option available for your country.
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Hill, C. W., Squires, C., and Carbon, J., J. Mol. Biol., 52, 557 (1970).
Carbon, J., Squires, C., and Hill, C. W., Cold Spring Harbor Symp. Quant. Biol., 34, 505 (1969).
Carbon, J., Squires, C., and Hill, C. W., J. Mol. Biol., 52, 571 (1970).
Hill, C. W., Foulds, J., Soil, L., and Berg, P., J. Mol. Biol., 39, 563 (1969).
Barrell, B. G., in Procedures in Nucleic Acid Research (edit. by Cantoni, G. L., and Davis, D. R.) (Harper and Row, New York, 1970).
Smith, J. D., Abelson, J. N., Clark, B. F. C., Goodman, H. M., and Brenner, S., Cold Spring Harbor Symp. Quant. Biol., 31, 479 (1966).
Abelson, J. N., Gefter, M. L., Barnett, L., Landy, A., Russell, R. L., and Smith, J. D., J. Mol. Biol., 47, 15 (1970).
Goodman, H. M., Abelson, J., Landy, A., Brenner, S., and Smith, J. D., Nature, 217, 1019 (1968).
Carbon, J., and Curry, J. B., J. Mol. Biol., 38, 201 (1968).
Yaniv, M., and Barrell, B. G., Nature, 222, 278 (1969).
Schulman, L. H., and Chambers, R. W., Proc. US Nat. Acad. Sci., 61, 308 (1968).
Yanofsky, C., Ito, J., and Horn, V., Cold Spring Harbor Symp. Quant. Biol., 31, 151 (1966).
Yaniv, M., and Barrell, B. G., Nature New Biology (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SQUIRES, C., CARBON, J. Normal and Mutant Glycine Transfer RNAs. Nature New Biology 233, 274–277 (1971). https://doi.org/10.1038/newbio233274a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/newbio233274a0
This article is cited by
-
The early history of tRNA recognition by aminoacyl-tRNA synthetases
Journal of Biosciences (2006)
-
A · T → C · G transversions and their prevention by the Escherichia coli mutT and mutHLS pathways
Molecular and General Genetics MGG (1989)
-
Mutagenicity and toxicity of chloroethylene oxide and chloroacetaldehyde
Experientia (1985)
-
Origin of the genetic code and specificity of tRNA aminoacylation. A testable model
Origins of Life (1984)
-
Characterization of missense suppressors of a double mutant of the tryptophan synthetase alpha chain of Escherichia coli
Molecular and General Genetics MGG (1978)