Abstract
THE temperate bacteriophage Mu-1 can induce mutations at many different loci in the genome of its host bacterium Escherichia coli K12 (see ref. 1). The mutations are assumed to arise by the insertion of Mu-1 DNA within the affected genes, as the sites of mutations are inseparable from the prophage sites by genetic criteria2, 3. It has been inferred therefore that Mu-1 can integrate at a very large number of chromosomal sites. This attribute of Mu-1 makes it distinct from other known temperate coliphages; the lambdoid phages attach at a particular site4, P2 phage attaches at a limited number of sites5, and P1 resides in the cell as a plasmid6.
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Application of the bacteriophage Mu-driven system for the integration/amplification of target genes in the chromosomes of engineered Gram-negative bacteria—mini review
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References
Taylor, A. L., Proc. US Nat. Acad. Sci., 50, 1043 (1963).
Martuscelli, J., Taylor, A. L., Cummings, D. J., Chapman, V. A., Delong, S., and Canedo, L., J. Virol., 8, 551 (1971).
Bukhari, A. I., and Taylor, A. L., J. Bact., 105, 844 (1971).
Campbell, A. M., Episomes (Harper and Row, New York, 1969).
Six, E., Virology, 29, 106 (1966).
Ikeda, H., and Tomizawa, J., Cold Spring Harbor Symp. Quant. Biol., 33, 791 (1968).
Taylor, A. L., and Trotter, C. D., Bact. Rev., 31, 332 (1967).
Zipser, D., Zabell, S., Rothman, J., Grodzicker, T., Wenk, M., and Novitski, M., J. Mol. Biol., 49, 251 (1970).
Zabin, I., and Fowler, A. V., in The Lactose Operon (edit. by Beckwith, J. R., and Zipser, D.), 27 (Cold Spring Harbor Laboratory, 1970).
Kennedy, E. P., in The Lactose Operon (edit. by Beckwith, J. R., and Zipser, D.), 49 (Cold Spring Harbor Laboratory, 1970).
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BUKHARI, A., ZIPSER, D. Random Insertion of Mu-1 DNA within a Single Gene. Nature New Biology 236, 240–243 (1972). https://doi.org/10.1038/newbio236240a0
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DOI: https://doi.org/10.1038/newbio236240a0
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