Abstract
Chromosomes from antigen stimulated B–cells from spleens of inbred mice have been separated using flow cytometry into 18 distinguishable peaks. Using locus–specific oligonucleotides and fluorescence in situ hybridization to banded metaphase spreads, 15 individual chromosomes were identified: 1, 2, 3, 6, 7, 8, 9, 11, 12, 16, 17, 18, 19, X and Y. The remaining six chromosomes, occurring as pairs in three peaks, 4 with 5, 10 with 13, and 14 with 15, were resolved by flow sorting chromosomes from mice carrying an appropriate homozygous translocation and 4, 5 and 14 have been isolated in this way. This is the first demonstration of how a complete set of mouse chromosome paints can be produced.
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Rabbitts, P., Impey, H., Heppell-Parton, A. et al. Chromosome specific paints from a high resolution flow karyotype of the mouse. Nat Genet 9, 369–375 (1995). https://doi.org/10.1038/ng0495-369
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DOI: https://doi.org/10.1038/ng0495-369
