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A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide

Nature Protocols volume 1, pages 2320–2325 (2006)Cite this article

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Abstract

We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract nucleic acids from plant tissues. In contrast to the original method, the modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. This protocol yields approximately 5–30 μg of total DNA from 200 mg of tissue fresh weight, depending on plant species and tissue source. It can be completed in as little as 5–6 h.

Note: In the version of this article initially published online, the name of the coauthor S Krasnyanski was misspelled as S Krasynanski. This error has been corrected in all versions of the article.

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Figure 1: Flow diagram to illustrate the major steps of the modified CTAB DNA isolation protocol.
Figure 2: Electrophoretic and restriction analyses of DNA extracted from leaf tissue of three different plant species.

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Change history

  • 31 January 2007

    In the version of this article initially published online, the name of the coauthor S Krasnyanski was misspelled as S Krasynanski. This error has been corrected in all versions of the article.

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Acknowledgements

This work was funded by grants from the National Science Foundation (520630 and 524026).

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Author notes

  1. G C Allen, M A Flores-Vergara, S Krasynanski, S Kumar and W F Thompson: All authors contributed equally to this work.

Authors and Affiliations

  1. Departments of Horticultural Science and Crop Science, Genetics and Crop Science, 1200 Partners II, Campus Box 7550, 840 Main Campus Drive, North Carolina State University, Raleigh, 27606-7550, NC, USA

    G C Allen

  2. Department of Plant Biology, Genetics and Crop Science, 1200 Partners II, Campus Box 7550, 840 Main Campus Drive, North Carolina State University, Raleigh, 27606-7550, NC, USA

    M A Flores-Vergara & S Kumar

  3. Department of Crop Science, Genetics and Crop Science, 1200 Partners II, Campus Box 7550, 840 Main Campus Drive, North Carolina State University, Raleigh, 27606-7550, NC, USA

    S Krasynanski

  4. Departments of Plant Biology, Genetics and Crop Science, 1200 Partners II, Campus Box 7550, 840 Main Campus Drive, North Carolina State University, Raleigh, 27606-7550, NC, USA

    W F Thompson

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Correspondence to G C Allen.

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Allen, G., Flores-Vergara, M., Krasynanski, S. et al. A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. Nat Protoc 1, 2320–2325 (2006). https://doi.org/10.1038/nprot.2006.384

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