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Proteome chips for whole-organism assays

Nature Reviews Molecular Cell Biology volume 7, pages 617–622 (2006)Cite this article

Abstract

Over the past 5 years, protein-chip technology has emerged as a useful tool for the study of many kinds of protein interactions and biochemical activities. The construction of Saccharomyces cerevisiae whole-proteome arrays has enabled further studies of such interactions in a proteome-wide context. Here, we explore some of the recent advances that have been made at the '-omic' level using protein microarrays.

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Figure 1: Schematic of proteome-chip construction and assay.
Figure 2: Examination of the yeast glycome.
Figure 3: Identification of DNA-binding sites of novel DNA-binding proteins.
Figure 4: Enriched modules of regulation discovered from phosphorylation data.

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Authors and Affiliations

  1. the Department of Molecular, Cellular and Developmental Biology, Yale University, 266 Whitney Avenue, New Haven, 06511, Connecticut, USA

    Li A. Kung & Michael Snyder

  2. Li A. Kung & Michael Snyder

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  1. Li A. Kung
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  2. Michael Snyder
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Dr. Snyder is a consultant with Invitrogen.

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FURTHER INFORMATION

Michael Snyder's homepage

Berkeley Drosophila Genome Project, Drosophila Gene Collection

Mammalian Gene Collection

Saccharomyces cerevisiae Phosphorylome Database

Open Biosystems Human ORF Collection

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Kung, L., Snyder, M. Proteome chips for whole-organism assays. Nat Rev Mol Cell Biol 7, 617–622 (2006). https://doi.org/10.1038/nrm1941

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