ABSTRACT
The present study investigates the molecular details of how arsenic trioxide inhibits preadipocyte differentiation and examines the role of Akt/PKB in regulation of differentiation and apoptosis. Continual exposure of arsenic trioxide, at the clinic achievable dosage that does not induce apoptosis, suppressed 3T3-L1 cell differentiation into fat cells by inhibiting the expression of PPARγ and C/EBPα and disrupting the interaction between PPARγ and RXRα, which determines the programming of the adipogenic genes. Interestingly, if we treated the cells for 12 or 24 h and then withdrew arsenic trioxide, the cells were able to differentiate to the comparable levels of untreated cells as assayed by the activity of GAPDH, the biochemical marker of preadipocyte differentiation. Long term treatment blocked the differentiation and the activity of GAPDH could not recover to the comparable levels of untreated cells. Continual exposure of arsenic trioxide caused accumulation in G2/M phase and the accumulation of p21. We found that arsenic trioxide induced the expression and the phosphorylation of Akt/PKB and it inhibited the interaction between Akt/PKB and PPARγ. Akt/PKB inhibitor appears to block the arsenic trioxide suppression of differentiation. Our results suggested that Akt/PKB may play a role in suppression of apoptosis and negatively regulate preadipocyte differentiation.
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INTRODUCTION
Arsenic, a natural constituent of soil and water, is a well-known human carcinogen 1, 2. Chronic exposure to inorganic forms of it from drinking water and air pollution can cause many types of cancers such as skin cancer, bladder cancer, liver cancer and kidney cancer. In addition to its carcinogenic effects, arsenic has been linked to type II diabetes mellitus 1 and cardiovascular diseases 2, for example atherosclerosis, hypertension 3, and blackfoot disease 4. The mechanisms of these effects of arsenic on the development of diseases are not known. But previous studies indicated that alteration of cell proliferation contributes to its carcinogenicity 5. On the other hand, arsenic has been used to treat cancers and it brings complete remission of acute promyeloid leukemia 6 . This could be due to the induction of differentiation at lower dose or induction of apoptosis or autophagic cell death at higher dose 7. Others and we have reported that arsenic at clinically achievable dosage induced apoptosis by activating mitochondrial dependent apoptosis 8, 9. It is not surprising that arsenic trioxide could disrupt multiple signaling pathways for mitogenesis, differentiation, apoptosis and cell cycle control 10, 11.
Arsenic has significant impact on the differentiation of cultured cells, including keratinocytes 12 and adipocytes 13. However, the molecular details for arsenic to inhibit differentiation are still not clear. It is known that the induction of terminal differentiation requires a comprehensive reprogramming of gene expression, and this could occur in parallel with withdrawal from the cell cycle. PPARγ and C/EBPα are two important transcription factors governing adipocyte differentiation. PPARγ is a ligand-activated transcription factor that belongs to the nuclear hormone receptor family. It needs to form an active heterodimer with RXRα (Rentinoid X Receptor) to bind to PPAR-response elements (PPRE), and stimulate transcription of the target genes. The cooperation of PPARγ and RXR drives the expression of a number of adipocytic genes for preadipocyte to differentiate and to maintain adipogenic phenotype 14. The serine-threonine protein kinase Akt/PKB, also known as Protein kinase B (PKB), is an important survival factor when cells were stimulated by a number of growth factors or cytokines. It is reported that Akt/PKB is a pivotal mediator of the pathway of insulin signaling that induce the differentiation of preadipocyte 15. Also, it is well documented that Akt/PKB play a critical role in regulating apoptosis. In this paper, we described that arsenic trioxide inhibited preadipocyte differentiation by downregulation of transcription factors of PPARγ and C/EBPα and the interaction between PPARγ and its ligand RXRα. We found that Akt/PKB was induced but the interaction between Akt/PKB and PPARγ was blocked by arsenic trioxide.
MATERIALS AND METHODS
Cell lines, antibodies and reagents
Mouse fibroblast line 3T3-L1 preadipocytes (ATCC CL173) were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco BRL) containing 10% fetal bovine serum (Hyclone), 100 U/ml penicillin and 0.1 mg/ml streptomycin (Gibco). To induce differentiation, 3T3-L1 preadipocytes were grown to confluence for two days. 2 d after confluence, cells were induced to differentiate (d 0) with addition of the standard differentiation inducing mix. Insulin (100 nM, Sigma), dexamethasone (0.25 μM, Sigma) and isobutylmethylxanthine (IBMX) (0.5 mM, Sigma) were added to the medium for the first 48h. From day 2 to 6, the medium was supplemented with 100 nM insulin only. The cells were then switched back to 10% fetal bovine serum from d 6 to 8. The cultures were replenished every 2 days. 3 μM arsenic trioxide was added into the medium 2 d prior to the induction and during the induction period. 5 μM Akt/PKB inhibitor (1L-6-Hydroxymethyl-chiro-inositol 2-(R)-2-O-methyl-3-O-octadecylcarbonate, Calbiochem) was added into the medium 2 d prior to the induction in the presence or absence of 3 μM arsenic trioxide.
Oil Red O staining
Dishes were washed three times with phosphate-buffered saline, fixed by 10% formalin in phosphate buffer for 1 h at room temperature. After fixation, cells were washed once with phosphate-buffered saline and stained with filtered Oil Red O stock solution (0.5 g of Oil Red O (Sigma) in 100 ml isopropyl alcohol) for 2 h at room temperature. Cells were washed twice with water, visualized and photographed.
Cell apoptosis assay
Cells were harvested and washed with PBS and then binding buffer. After that, cells were stained with 25 ng/mL Annexin V-FITC at 37°C for 10min, and stained with PI just before detection. Then Annexin-V positive apoptotic cells was determined by a BD FACScan as previously described 8.
Cell cycle assay
Cell synchronization
Cells were incubated at the density of 2.5×105 cells/150mm2 in 10% FBS DMEM. 24h later, cells were washed with PBS and then starved in DMEM containing 0.1% FBS for 24h to synchronize. Then cell cycle was initiated by replacement of the starvation medium with the full medium (DMEM with 10% FBS). At the same time 3 μM arsenic trioxide was added into the medium.
Flow cytometric analysis of cell cycle
Cell cycle distribution was determined by using fluorescence activated cell sorting (FACS). Briefly, cells were harvested and washed twice with PBS. After centrifugation, pellets were re-suspended in 500 μl PBS, added drop by drop into 5 ml cold (-20° C) ethanol and fixed at 4° C overnight. Before staining, the ethanol was removed and cells were washed twice with 1% BSA (in PBS). Then cells were re-suspended in 400 μl PBS containing 1% BSA. 50 μl 500 μg/ml PI and 50 μl 10 mg/ml boiled RNase A were added and cells were incubated at 37° C for 30 min before determination. The proportion of cells in each phase of cell cycle was determined by a BD FACScan.
Determination of enzyme activity of GAPDH
Cells were washed twice with ice-cold PBS and STEM (250 μM Sucrose, 10 mM Tris-Hcl (PH7.4), 1 mM EDTA, 1 mM 2-Mercaptoethanol), and were scraped down to centrifuge. Then cells were re-suspended with 200 μl STEM, fractioned by supersonic and centrifuged at 4°C, 12,000 rpm for 30 min. The quantity of protein in supernatant was determined and adjusted to the same level. 240 μl reaction buffer (100 mM Triethanolamine (PH 7.7), 0.25 mM EDTA, 1 mM 2-Mercaptoethanol, 0.2 mM NADH), 160 μl Dihydroxyacetone phosphate and 400 μl STEM plus supernatant were added to a 1 ml quartzose cup. Change of absorbance at 340 nm was recorded by using a Beckman Spectrophotometer. The activity of GAPDH were computed by the initial velocity of the reaction and showed as nmol/min/mg protein.
Western blotting
Cells were harvested and washed twice with PBS, then were lysed with lysis buffer at 4° C for 30 min. cells were centrifuged at 12,000 rpm, 4° C for 15min. After adjusting the protein concentrations, lysates were boiled with loading buffer at 100° C for 10 min. Lysates were run in 12% polyacrylamide-SDS gels, and transferred to nitrocellulose. The nitrocellulose blots were blocked with phosphate-buffered saline containing 5% non-fat milk and 0.1% Tween 20 and then treated with antibodies that recognize p21, PPARγ, C/EBPα or Akt/PKB, respectively.
Immunoprecipitation
Cells were harvested and washed twice with PBS, then were lysed with lysis buffer at 4° C for 30 min. Cells were centrifuged at 12,000 rpm for 15min at 4° C. Supernatants were carefully collected without disturbing the pellet and transferred to a clean tube. The quantity of protein in them was determined and adjusted to the same level. 2 μg PPARγ monoclonal antibody and 500 μl lysis buffer were added into the supernatants if it contains 500 μg proteins and incubated at 4° C overnight. Protein G-argarose was washed thrice with lysis buffer and added 20 μl per sample. Then the proteins were incubated at 4° C for 2 h. After thrice washing with lysis buffer, proteins were boiled with loading buffer at 100° C for 10 min. Then western blotting was performed and antibodies such as RXRα, Akt/PKB were used.
RESULTS
Clinic achievable dosage of arsenic trioxide could not induce apoptosis but potently inhibited the differentiation of preadipocyte 3T3-L1
We first examined the effect of arsenic trioxide on cell apoptosis and found that 3 μM arsenic trioxide could not induce apoptosis of 3T3-L1 preadipocyte (Fig. 1A) although higher dosage induced cell death (data not shown). We did not detect measurable caspase activity after the treatments with clinic achievable dosage (data not shown). We observed that continual exposure of the cells with 3 μM arsenic trioxide potently inhibited differentiation of 3T3-L1 cells as assayed by Oil Red O staining. As shown in Fig. 1D, cells treated with insulin containing differentiation mix were induced to differentiate and obtained morphology with microscopically visible droplets of lipids. In contrast, under the identical conditions, cells grown in 3 μM arsenic trioxide or 0.5 μM VP16, a DNA damage agent, did not show lipid accumulation (Fig. 1E and F). We further examined the biochemical marker of preadipocyte differentiation, the appearance of glycerophosphate dehydrogenase (GAPDH). As shown in Fig. 1G, the activity of GAPDH was strongly inhibited by 3 μM arsenic trioxide. Interestingly, the cells can continue to differentiate to the comparable levels if we treated the cells for 12 or 24 h and then withdrew arsenic trioxide (Fig. 1H). If the cells were treated for 48h with arsenic trioxide, the differentiation was markedly blocked and the activity of GAPDH could not recover to the comparable levels of untreated cells.
Arsenic trioxide induced slight G2 accumulation of cell cycle and p21 expression
Because arsenic trioxide and VP16 are both cytotoxic agents, commonly used clinically for treatments of cancers, we next checked their effects on cell cycle of 3T3-L1. Cells were treated with arsenic trioxide after 24 h serum starvation, and the cell cycle was examined by flow cytometry after PI staining. As shown in Fig. 2A, there was an increase of S phase at 24h and accumulation of G2/M phase at 48h after arsenic trioxide treatment, whereas VP16 treated cells were arrested at G2/M 24h after treatment (Fig. 2B and 2C), suggesting that these two agents has distinct effects on cell cycle. At the same time, p21 were increased following arsenic trioxide treatments (Fig. 2D).
Arsenic trioxide inhibited the expression of PPARγ and C/EBPα
To understand the molecular mechanisms of arsenic inhibition of preadipocyte differentiation, we next examined the protein levels of PPARγ and C/EBPα following the induction of differentiation and the treatment of the cytotoxic agent. As shown in Fig. 3, expression of PPARγ and C/EBPα were significantly reduced in arsenic trioxide treated cells in comparison with that of control. There was significant reduction of both PPARγ1 and PPARγ2 expression although there was a fluctuation of PPARγ1 expression following the treatments. These results indicate that arsenic trioxide inhibits lipid accumulation and preadipocyte differentiation by downregulation of the expression of adipocyte transcription factors during differentiation.
Arsenic trioxide inhibited the interaction between PPARγ and RXRα
Because the interaction between PPARγ and RXRα is critical for the activity of the transcription complex, we examined the expression of RXRα and interaction between the two proteins after the treatment of 3 μM arsenic trioxide and 0.5 μM VP16. Our result showed that the treatment of arsenic trioxide also inhibited the expression of RXRα (Fig. 4A). Importantly, we found that the interaction between PPARγ and RXRα was reduced when treated with arsenic trioxide as assayed by immunoprecipitation (Fig. 4B). Another cytotoxic agent VP16 had minimal effect on the interaction of PPARγ and RXRα.
Arsenic trioxide could inhibit the interaction between PPARγ and Akt/PKB
Having shown that arsenic trioxide strongly suppressed differentiation by disrupting signaling PPARγ/RxRα pathway, we next examined if Akt/PKB may be involved in mediating the survival signal even when cells were treated with arsenic trioxide. We found that the treatment of arsenic trioxide increased the expression of Akt/PKB compared to that of control which was treated with differentiation mix. Arsenic trioxide significantly enhanced the phosphorylation of Akt/PKB, thereby its activation (Fig. 5A), although the expression of PPARγ, C/EBP and RXR was reduced under identical conditions. Interestingly, we found that Akt/PKB inhibitor could counteract the repression effect of arsenic trioxide on the differention of 3T3-L1. When the 3T3-L1 cells were treated with arsenic trioxide and Akt/PKB inhibitor together, there are more than 90% of cells accumulate droplets of lipids (see Fig. 5C and D). Furthermore, we showed that Akt/PKB interacted with PPARγ directly and arsenic trioxide could suppress the interaction between Akt/PKB and PPARγ (Fig. 5B). Our results suggest that Akt/PKB could negatively regulate preadipocyte differentiation and may function to suppress apoptosis in the cells when treated with arsenic trioxide.
DISCUSSION
The current study examined the molecular details of the inhibitory effects of arsenic trioxide on cell differentiation and apoptosis. We found that arsenic trioxide could inhibit the expression of the PPARγ and RXRα and disrupt the interaction between them. We showed that Akt/PKB expression and its phosphorylation are induced upon arsenic trioxide treatment. Terminal differentiation is usually associated with the long term survival. Apart from the induction of the differentiation programme, insulin also activates survival signals. Of note, Akt/PKB, which was found to be a critical molecule for cell survival, is significantly induced by insulin. We found that Akt/PKB expression was induced and it was activated as revealed by phosphorylation upon the treatment of arsenic trioxide, even when the cell differentiation is significantly suppressed. It is interesting to note that Akt/PKB can directly interact with PPARγ and arsenic trioxide can inhibit the interaction between them. The reduced interaction is associated with the inhibition of cell differentiation. Of note, we found that Akt/PKB inhibitor could counteract, at least in part, the repression effect of arsenic trioxide on the differention of 3T3-L1 while the inhibitor itself has minimal effects on cell differentiation and cell death. This indicates that the repression effect of arsenic trioxide on the differentiation was depended on the activation of Akt/PKB. This is in agreement with previous report that Akt/PKB-α plays an important role in the regulation of preadipocyte growth and proliferation, and it is down-regulated upon differentiation 16. Our results are also in agreement with wealth information that Akt/PKB functions to suppress apoptosis. It is found that Akt/PKB could phosphorylate Bad, a Bcl-2 family protein to suppress apoptosis 17. Alternatively, Akt/PKB could inhibit Bax conformation change, critical events for apoptosis 18. It should be noted that arsenic trioxide activate a number of signaling pathways, such as MAPK, ERK and JNK or suppress certain signaling pathways 19, 20. The molecular details of the network of different signaling molecules to regulate cell differentiation need to be further investigation.
In addition to show that it inhibits the interaction between Akt/PKB and PPARγ, we found that arsenic trioxide disrupts the interaction between PPARγ and RXR, critical transcriptional factors functions together to regulate adipogenic genes expression. These results extended previous findings which suggested that sodium arsenite could inhibit the differentiation of another preadipocyte cell line, C3H 10T1/2 by inhibiting the expression of PPARγ and C/EBPα 21. It appears that the inhibition of the activity of GAPDH by arsenic trioxide is reversible and preadipocyte differentiation reached the comparable levels between control cells and cells exposed to the short exposure (12, 24 h). There may be a critical decision-making time for the cells to decide their fates between 24 and 48 h. We observed that there was an accumulation of the cells at G2 phase and the increased expression of p21, at 48 h. p21 was also implicated in regulating cell survival. Altiok and his colleague found that PPARγ initiates withdrawal from the cell cycle during adipogenesis 22. In smooth muscle cells, PPARγ activation results in accumulation of cells in G0/G1 23. Our results showed that arsenic trioxide decreased the expression of PPARγ, suggesting that it is not likely to be responsible for G2 accumulation. Our results also is in contrast to the finding that sodium arsenite inhibited the expression of p21 21. Other report suggests that arsenite induced p27, which is also related to cell cycle entry 24. This warrants further study of the determination of cell fates under arsenic stress conditions.
In summary, current study corroborates the earlier report that arsenic inhibits differentiation of preadipocytes into adipocytes. We showed that arsenic disrupts adipogenesis via perturbation of the interaction of PPARγ and RXRα pathway. Importantly, our data suggest that Akt/PKB could be a major survival signal and negatively regulate cell differentiation. Our results may be useful for understanding of effects of arsenic trioxide in cell death and differentiation.
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Acknowledgements
This work is supported by grants from National Nature Science Foundation of China (No.30000083). Quan CHEN's laboratory was supported by grants “Knowledge Innovation Key Project” (kscx2-sw-2010) of Chinese Academy of Sciences, and the National Basic Research Program of China (973 program project, No.2002CB513100) and National Fund for Outstanding Young Scholars from NSFC (30325013) awarded to Quan CHEN.
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WANG, Z., JIANG, C., LIU, L. et al. The role of Akt on Arsenic trioxide suppression of 3T3-L1 preadipocyte differentiation. Cell Res 15, 379–386 (2005). https://doi.org/10.1038/sj.cr.7290305
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DOI: https://doi.org/10.1038/sj.cr.7290305
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