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Induction of stable RNA interference in mammalian cells

Gene Therapy volume 13, pages 503–508 (2006)Cite this article

Abstract

Over the last years, RNA interference (RNAi) has become a widely used technique that permits the knock-down, and hence functional analysis, of individual genes in vertebrate cells. However, the high failure rate of the RNA molecules used in RNAi experiments continues to be a problem. In this paper, I describe a set of design criteria, experimental steps and expression vectors that can facilitate the effective knock-down of almost any vertebrate gene product in cultured cells or in experimental animals.

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Acknowledgements

I thank Dr Alexandra Schäfer for assistance with the figures used in this manuscript. This work was supported by Grant GM071408 from the National Institutes of Health.

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  1. Center for Virology and Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA

    Bryan R Cullen

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  1. Bryan R Cullen
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Correspondence to Bryan R Cullen.

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Cullen, B. Induction of stable RNA interference in mammalian cells. Gene Ther 13, 503–508 (2006). https://doi.org/10.1038/sj.gt.3302656

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