Abstract
A total organophosphorus pesticide exposure study was conducted in Washington State in 1998 in a sample population of 13 children aged 2.5–5.5 years. The children were roughly split between rural and suburban populations and had been previously identified as having potentially elevated organophosphorus pesticide exposures. One component of the study was urine collection and analysis. Urine samples were collected from each subject up to four times in 24 h in two different seasons. Samples were collected at specific time points: before bed, first morning void, after lunch, and before dinner. Urine samples were analyzed initially for the six nonspecific dialkylphosphate (DAP) metabolites and subsequently for eight specific metabolites including malathion dicarboxylic acid (MDA), 3,5,6-trichloro-2-pyridinol (TCPy), and paranitrophenol (PNP). Relatively large percentages of the urine samples contained quantifiable amounts of two of the nonspecific DAP metabolites (DMTP—97%; DETP—67%), and three of the specific metabolites (MDA (71%), TCPy (79%), and PNP (96%)). A percent deviation analysis was employed to determine which of the spot sample time points was the best predictor of the estimated volume-weighted daily average. Of the four spot samples collected, first morning void samples were consistently found to be the best predictors of weighted-average daily metabolite concentration. This finding also held when the data were creatinine-adjusted. The results of this analysis suggest that if spot sampling is to be conducted as part of a biological monitoring study, first morning void samples should be preferentially collected.
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Acknowledgements
This work was supported primarily by the U.S. EPA Science to Achieve Results (STAR) program (R819186). Additional support came from the U.S. EPA/NIEHS Child Health Centers Program (R826886/PO1ES09601) and the NIOSH Agricultural Centers Program (U07/CCU012926). We thank Tom Moate for his analytical work and our subjects for their participation. These findings do not necessarily reflect the views of the funding agencies and no official endorsement should be inferred.
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Kissel, J., Curl, C., Kedan, G. et al. Comparison of organophosphorus pesticide metabolite levels in single and multiple daily urine samples collected from preschool children in Washington State. J Expo Sci Environ Epidemiol 15, 164–171 (2005). https://doi.org/10.1038/sj.jea.7500384
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DOI: https://doi.org/10.1038/sj.jea.7500384
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