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  • Review Article
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Options available—from start to finish—for obtaining data from DNA microarrays II

Nature Genetics volume 32, pages 481–489 (2002)Cite this article

Abstract

Microarray technology has undergone a rapid evolution. With widespread interest in large-scale genomic research, an abundance of equipment and reagents have now become available and affordable to a large cross section of the scientific community. As protocols become more refined, careful investigators are able to obtain good quality microarray data quickly. In most recent times, however, perhaps one of the biggest obstacles researchers face is not the manufacture and use of microarrays at the bench, but storage and analysis of the array data. This review discusses the most recent equipment, reagents and protocols available to the researcher, as well as describing data analysis and storage options available from the evolving field of microarray informatics.

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Figure 1: Comparison of commercial slides for printing of cDNA material.
Figure 2: Output from the local mean normalization step of SNOMAD normalization.

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Acknowledgements

We are grateful to M. Murphy, K. Belfrage, S. Katsabanis and D. Diyagama for their assistance with all aspects of microarray production and use and to Cold Spring Harbour Laboratory Press, in particular K. Jaansen, J. Sambrook and the contributors to Microarrays, A Molecular Cloning Manual, for granting permission to reproduce tables included in this manuscript. The PMCI microarray facility was established with the generous support of the Ian Potter Foundation and the Wellcome Trust. The ongoing operation of the Facility is supported by a Science Technology Innovation Initiative Grant from the State Government of Victoria, Australia.

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  1. The Ian Potter Foundation Centre for Cancer Genomics and Predictive Medicine and The Trescowthick Research Laboratories, Peter MacCallum Cancer Institute, Locked Bag 1, A'Beckett Street, Melbourne, 8006, Victoria, Australia

    Andrew J. Holloway, Ryan K. van Laar, Richard W. Tothill & David D.L. Bowtell

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Correspondence to David D.L. Bowtell.

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Holloway, A., van Laar, R., Tothill, R. et al. Options available—from start to finish—for obtaining data from DNA microarrays II. Nat Genet 32 (Suppl 4), 481–489 (2002). https://doi.org/10.1038/ng1030

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