Abstract
Background:
Novel predictors of prognosis and treatment response for prostate cancer (PCa) are required to better individualize treatment. Single-nucleotide polymorphisms (SNPs) in four genes directly (XRCC5 (X-ray repair complementing defective repair in Chinese hamster cells 5) and XRCC6 (X-ray repair complementing defective repair in Chinese hamster cells 6)) or indirectly (PARP1 and major vault protein (MVP)) involved in non-homologous end joining were examined in 494 Spanish PCa patients.
Methods:
A total of 22 SNPs were genotyped in a Biotrove OpenArray NT Cycler. Clinical tumor stage, diagnostic PSA serum levels and Gleason score at diagnosis were obtained for all participants. Genotypic and allelic frequencies were determined using the web-based environment SNPator.
Results:
(XRCC6) rs2267437 appeared as a risk factor for developing more aggressive PCa tumors. Those patients carrying the GG genotype were at higher risk of developing bigger tumors (odds ratio (OR)=2.04, 95% confidence interval (CI) 1.26–3.29, P=0.004), present higher diagnostic PSA levels (OR=2.12, 95% CI 1.19–3.78, P=0.011), higher Gleason score (OR=1.65, 95% CI 1.01–2.68, P=0.044) and D’Amico higher risk tumors (OR=2.38, 95% CI 1.24–4.58, P=0.009) than those patients carrying the CC/CG genotypes. Those patients carrying the (MVP) rs3815824 TT genotype were at higher risk of presenting higher diagnostic PSA levels (OR=4.74, 95% CI 1.40–16.07, P=0.013) than those patients carrying the CC genotype. When both SNPs were analyzed in combination, those patients carrying the risk genotypes were at higher risk of developing D’Amico higher risk tumors (OR=3.33, 95% CI 1.56–7.17, P=0.002).
Conclusions:
We believe that for the first time, genetic variants at XRCC6 and MVP genes are associated with risk of more aggressive disease, and would be taken into account when assessing the malignancy of PCa.
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Acknowledgements
We thank the technical support from the Immunology Department (Hospital Universitario de Gran Canaria Dr Negrín) staff: Nereida González-Quevedo and Yanira Florido-Ortega. This work was subsidized by grants from the Instituto de Salud Carlos III (Ministerio de. Economía y Competitividad from Spain), ID: PI12/01867 and PI13/00412; and Instituto Canario de Investigación del Cáncer (ICIC-GR-F-14/11). Almudena Valenciano has a grant from the Instituto Canario de Investigación del Cáncer (ICIC). The founders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.
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Henríquez-Hernández, L., Valenciano, A., Foro-Arnalot, P. et al. Association between single-nucleotide polymorphisms in DNA double-strand break repair genes and prostate cancer aggressiveness in the Spanish population. Prostate Cancer Prostatic Dis 19, 28–34 (2016). https://doi.org/10.1038/pcan.2015.63
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DOI: https://doi.org/10.1038/pcan.2015.63