Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

Comment on: “Hypoxia differently modulates the release of mitochondrial and nuclear DNA”

British Journal of Cancer volume 124, pages 2035–2036 (2021)Cite this article

Subjects

We read with interest the article by Otandault and colleagues investigating the effects of hypoxia on the release of nuclear-derived cell-free DNA (NcfDNA) and mitochondrial-derived cell-free DNA (McfDNA) in laboratory cancer models.1 We agree that research into the physiology of NcfDNA and McfDNA release is of paramount importance for designing future ‘liquid biopsy’ technologies and gaining understanding of the underlying cancer biology.

Otandault et al. investigated NcfDNA and McfDNA release dynamics in hypoxia using in vitro (colorectal cancer cell-lines) and in vivo (TC1-engrafted mice) models. The study offered fascinating insights, suggesting differing mechanisms of NcfDNA release and McfDNA release in vitro.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Otandault, A., Abraham, J. D., Al Amir Dache, Z., Khalyfa, A., Jariel-Encontre, I., Forné, T. et al. Hypoxia differently modulates the release of mitochondrial and nuclear DNA. Br. J. Cancer 122, 715–725 (2020).

    Article  CAS  Google Scholar 

  2. Cox, D. R. A., Wong, B. K. L., Yang, L., Yoshino, O., Testro, A., Muralidharan, V. et al. High speed centrifugation before frozen storage of plasma is critical for quantitative analysis of mitochondrial-derived cell free DNA. Clin. Chem. 66, 1111–1114 (2020).

    Article  Google Scholar 

  3. Jirouskova, M., Shet, A. S. & Johnson, G. J. A guide to murine platelet structure, function, assays, and genetic alterations. J. Thromb. Haemost. 5, 661–669 (2007).

    Article  CAS  Google Scholar 

  4. Meddeb, R., Pisareva, E. & Thierry, A. R. Guidelines for the preanalytical conditions for analyzing circulating cell-free DNA. Clin. Chem. 5, 623–633 (2019).

    Article  Google Scholar 

  5. Boudreau, L., Duchez, A. C., Cloutier, N., Soulet, D., Martin, N., Bollinger, J. et al. Platelets release mitochondria serving as substrate for bactericidal group IIA-secreted phospholipase A2 to promote inflammation. Blood 124, 2173–2183 (2014).

    Article  CAS  Google Scholar 

  6. McDonald, T. P., Cottrell, M. & Clift, R. Effects of short-term hypoxia on platelet counts of mice. Blood 51, 165–175 (1978).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. These authors jointly supervised this work: Daniel Cox, Alexander Dobrovic.

Authors and Affiliations

  1. School of Cancer Medicine, La Trobe University, Bundoora, VIC, Australia

    Boris K. L. Wong

  2. Translational Genomics and Epigenomics Laboratory, Department of Surgery – Austin Precinct, University of Melbourne, Melbourne, VIC, Australia

    Boris K. L. Wong, Fan Zhang, Alexander Dobrovic & Daniel R. A. Cox

  3. Pathology Department, St Vincent’s Hospital Melbourne, Melbourne, VIC, Australia

    Hongdo Do

  4. Liver Transplant Unit, Department of Gastroenterology & Hepatology, Austin Hospital, Melbourne, VIC, Australia

    Adam Testro

  5. Department of Surgery – Austin Precinct, The University of Melbourne, Melbourne, VIC, Australia

    Vijayaragavan Muralidharan & Daniel R. A. Cox

  6. HPB & Transplant Surgery Unit, Department of Surgery, Austin Hospital, Melbourne, VIC, Australia

    Vijayaragavan Muralidharan & Daniel R. A. Cox

Authors
  1. Boris K. L. Wong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hongdo Do
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Adam Testro
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Vijayaragavan Muralidharan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Alexander Dobrovic
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Daniel R. A. Cox
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

B.K.L.W., D.R.A.C., F.Z. and H.D.: drafted and approved the manuscript prior to submission. A.T., V.M. and A.D.: provided critical revision of the manuscript, approved the manuscript prior to submission, and supervised the work.

Corresponding author

Correspondence to Daniel R. A. Cox.

Ethics declarations

Ethics approval and consent to participate

N/A.

Consent for publication

This article does not contain any individual’s identifiable data in any form. None of the material has been published or is under consideration elsewhere, including online journals. All authors have contributed to and agreed on the content of the manuscript.

Data availability

N/A.

Competing interests

The authors declare no competing interests.

Funding information

B.K.L.W. is the recipient of the Full-Fee Research Scholarship and Postgraduate Research Scholarship (La Trobe University). D.R.A.C. is the recipient of the Eric Bishop Research Scholarship (Royal Australasian College of Surgeons), the Ashley Johnson Scholarship (Victorian Liver Transplant Unit) and the Research Training Program Scholarship (University of Melbourne).

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wong, B.K.L., Zhang, F., Do, H. et al. Comment on: “Hypoxia differently modulates the release of mitochondrial and nuclear DNA”. Br J Cancer 124, 2035–2036 (2021). https://doi.org/10.1038/s41416-021-01287-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41416-021-01287-z

Search

Advanced search

Quick links