Abstract
Serum amyloid P component (SAP, also known as Pentraxin 2; APCS gene) is a component of the humoral arm of innate immunity involved in resistance to bacterial infection and regulation of tissue remodeling. Here we investigate the role of SAP in antifungal resistance. Apcs−/− mice show enhanced susceptibility to A. fumigatus infection. Murine and human SAP bound conidia, activate the complement cascade and enhance phagocytosis by neutrophils. Apcs−/− mice are defective in vivo in terms of recruitment of neutrophils and phagocytosis in the lungs. Opsonic activity of SAP is dependent on the classical pathway of complement activation. In immunosuppressed mice, SAP administration protects hosts against A. fumigatus infection and death. In the context of a study of hematopoietic stem-cell transplantation, genetic variation in the human APCS gene is associated with susceptibility to invasive pulmonary aspergillosis. Thus, SAP is a fluid phase pattern recognition molecule essential for resistance against A. fumigatus.
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Introduction
The innate immune system represents the first line of resistance against pathogens and a key determinant in the activation and orientation of adaptive immunity through the complementary activities of a cellular and humoral arm1. Cell-associated innate immune molecules sense pathogen-derived agonists leading to activation of different inflammatory pathways2. Humoral pattern recognition molecules (PRMs) are essential components of the innate immune response sharing functional outputs with antibodies3, including opsonisation, regulation of complement activation, agglutination, and neutralization1. Humoral PRMs in turn interact with and regulate cellular effectors1,3,4,5,6. Components of humoral innate immunity include complement components, ficolins, collectins, and pentraxins1,7,8.
Pentraxins are an ancient group of proteins evolutionarily conserved from arachnids and insects to humans characterized by the presence of a 200 amino acid (aa) pentraxin domain and a pentraxin signature (HxCxS/TWxS, x = any aa)7,8. CRP (also called PTX1) and SAP (PTX2; APCS gene) are the classic short pentraxins. CRP and SAP are acute phase response proteins produced in the liver in response to infection and inflammatory cytokines8,9,10.
SAP has complex biological functions that include regulation of matrix formation and resistance to infectious agents8,10. These general functions are shared by other pentraxins11,12,13,14. SAP was found to bind viruses (e.g., influenza)15, bacteria (e.g., Streptococcus pneumoniae, Staphylococcus aureus, mycobacteria)16,17 and malaria parasites18. Pentraxins, including SAP, behave as “ante-antibodies”, activating complement and interacting with Fcγ receptors (Rs)1,2,3. SAP regulates complement activation by interacting with C1q19, members of the ficolin family20 and the complement regulator C4-binding protein (C4BP)21. Moreover SAP interacts with FcγRs and has opsonic activity3,22,23,24,25. In in vitro and in vivo models of infection, SAP has been reported to have dual influence on antimicrobial resistance depending on the species and experimental conditions. For instance, interaction of SAP with S. pyogenes, Neisseria meningitides and some variants of Escherichia coli led to decreased phagocytosis and killing by macrophages and inhibition of complement activation26. Apcs−/− mice showed better survival in experimental infections with S. pyogenes and E. coli26.
SAP plays an important role in the regulation of extracellular matrix deposition. SAP is an important component of amyloidosis, where it promotes persistence of amyloid fibrils through binding and consequent stabilization27,28. A specific antibody against SAP promotes clearance of amyloid deposits in patients with systemic amyloidosis29. Moreover, SAP has been shown to inhibit lung fibrosis in preclinical models by regulating macrophage polarization30,31,32,33.
SAP has entered early clinical assessment for the treatment of lung fibrosis10. In a randomized clinical trial, use of recombinant and identical to the endogenous human SAP (PRM-151) was associated with reduction in fibrocytes in pulmonary fibrosis patients34. A Phase 2 study trial of PRM-151, a novel anti-fibrotic immunomodulator, was reported in patients with Idiopathic Pulmonary Fibrosis (IPF)35 and in a 28-week Phase 2 trial, infusions of SAP improved lung function36,37 by inhibiting alternative activation of macrophages and fibrocyte differentiation31,32.
The distant relative of SAP, PTX3, has been shown to play an essential role in resistance against A. fumigatus and related fungi. Strong genetic evidence is also consistent with a key role of PTX3 in resistance against A. fumigatus in humans in different clinical settings38,39,40,41,42,43,44,45,46,47. Given the PTX3 precedent, it was important to assess the role of SAP in resistance against A. fumigatus. Here we report that SAP was non-redundant for resistance against A. fumigatus in mice. SAP-promoted phagocytosis of conidia involved activation of the complement cascade. In immunosuppressed mice, SAP administration resulted in protection against A. fumigatus. In the context of hematopoietic stem-cell transplantation, the APCS locus emerged as a novel risk factor regulating susceptibility to invasive pulmonary aspergillosis (IPA). Thus, SAP is a fluid phase PRM that plays an essential role in resistance against A. fumigatus infection and has therapeutic potential against fungal infections.
Results
SAP is essential for resistance to A. fumigatus infection
Apcs−/− mice were used to assess the role of this molecule in resistance against A. fumigatus pulmonary infection. As shown in Fig. 1a, b, where two doses of conidia (1 × 108 or 5 × 107) were injected intratracheally (i.t.), Apcs−/− mice showed increased susceptibility to infection. Apcs−/− mice showed lethal infection with a median survival time (MST) of 3 days compared to MST > 10 days of wt, both when 1 × 108 (Fig. 1a) or 5 × 107 (Fig. 1b) conidia were used. Actually, 89.9% (8/9) (Fig. 1a) and 44.4% (5/9) (Fig. 1b) of Apcs−/− mice succumbed on day 3 compared to 23.8% (2/9) or 0% (0/6) of wt mice. At the end of the experiment (monitoring until day 10), 11.1% (1/9) and 33.3% (3/9) of Apcs−/− mice survived to infection compared to 55.6% (5/9) or 83.3% (5/6) of wt mice, at 1 × 108 (Fig. 1a) or 5 × 107 (Fig. 1b) conidia (P < 0.05). Figure 1 shows one experiment out of 2 (1 × 108) or 5 (5 × 107) performed. Supplementary Table 1 summarizes cumulative results obtained in experiments using 5 × 107 conidia. Similar results were obtained in pulmonary infections by different clinically relevant species of the Trichocomaceae family, such as A. flavus (5 × 107) (Supplementary Fig. 1) and A. terreus (1 × 108) (Supplementary Fig. 2). Although A. terreus showed low pathogeniticy in mice even with the highest dose of injected conidia (1 × 108), differences in survival (90.9%, 10/11 of survived wt mice at 1 × 108 conidia compared to 61.5%, 8/13 of Apcs−/− mice; P = 0.09) (Supplementary Fig. 2a) and fungal burden in the lung (P = 0.002) (Supplementary Fig. 2b) indicated augmented susceptibility associated with SAP-deficiency. No relevance of SAP was observed in a model of fungal dissemination using Candida albicans (i.v., 5 × 106) (wt, 50.0% vs. Apcs−/−, 58.3% of survived mice) (Supplementary Fig. 3).
In an effort to assess whether the phenotype observed in Apcs−/− mice was indeed related to SAP deficiency48, recombinant murine SAP was used. Pre-opsonisation of A. fumigatus conidia with recombinant murine SAP rescued the susceptibility of Apcs−/− mice to infection, without affecting the resistance of wt mice (Fig. 1c). Indeed, 69% (9/13) of SAP-treated Apcs−/− mice resisted to infection (MST > 10 days; P < 0.0001) compared to 0% (0/14) of Apcs−/− mice treated with vehicle (MST 3 days). Survival was similar in wt groups (wt: 80%, 8/10; MST > 10 days; SAP-treated wt: 70%, 7/10; MST > 10 days). Similar results were observed when murine SAP (50 µg/mouse) was injected intravenously (i.v.) (Fig. 1d).
SAP-deficiency was associated with defective recruitment of neutrophils in response to A. fumigatus, as assessed by cell counts (Supplementary Fig. 4a) (P = 0.008; 16 h) and levels of myeloperoxidase (MPO) (Apcs−/−, 449 ± 28 vs. wt, 564 ± 16 ng/ml; P = 0.008; 16 h) in bronchoalveolar lavage fluids (BALFs) (Supplementary Fig. 4b), and lower levels of C5a (Apcs−/−, 22.7 ± 1.7 vs. wt, 31.5 ± 1.8 ng/ml; P = 0.004; 16 h) (Supplementary Fig. 4b). Decreased C3d in lung lysates was also observed (P = 0.03) (Supplementary Fig. 4c). Murine SAP pre-opsonisation restored the levels of MPO and the number of neutrophils in BALFs of Apcs−/− mice (16 h) (Supplementary Fig. 5), hence suggesting that SAP-opsonized conidia trigger an inflammatory response in the lung essential for resistance to fungus.
Mechanism of SAP-mediated resistance to A. fumigatus
Injection of A. fumigatus conidia increased the circulating levels of SAP (0.55 ± 0.43 µg/ml; n = 3) at 4 (2.63 ± 1.02 µg/ml, n = 4) and 16 h (22.12 ± 8.50 µg/ml, n = 3) comparably to those observed after LPS administration (28.47 ± 11.43 µg/ml, n = 3; LPS, 0.8 mg/kg, 16 h) (Fig. 2a). In lungs (n = 3; 4 h), SAP localized in areas of cell recruitment and complement deposition closely associated with conidia (Fig. 2b).
Neutrophils are major players of resistance against A. fumigatus49. Therefore, we examined neutrophil phagocytosis of A. fumigatus in BALFs of Apcs−/− mice. As shown in Fig. 2c, d, in the lungs of Apcs−/− mice we observed a 6-fold increase of A. fumigatus CFU [Apcs−/−, median, 1.9 × 108, interquartile range (IQR) 3.0 × 108−1.6 × 108 vs. wt, 4.0 ± x 107, IQR 6.8 × 107−4.5 × 107; P < 0.0001] (Fig. 2c) and reduced phagocytosis by neutrophils (Apcs−/−, 28.74 ± 3.96% vs. wt, 42.53 ± 6.39%; P = 0.05) (Fig. 2d).
Based on these in vivo results, the interaction of SAP with conidia and its impact on phagocytosis and complement activation was examined in vitro. Recombinant murine SAP bound viable dormant, swollen and germinated conidia, as assessed by FACS (Fig. 2e) and microscopy (Fig. 2f). A similar binding was observed for A. flavus and A. terreus, but not for Candida albicans (Supplementary Fig. 6a–c). Binding on A. fumigatus was competed by human SAP, but not by CRP (Fig. 2e). Similarly, PTX3 competed binding (Supplementary Fig. 6d), thus indicating that the binding site(s) on A. fumigatus is (are) conserved between mouse and human SAP and PTX3.
In whole blood, phagocytosis by neutrophils was reduced in Apcs−/− mice, both at 1 (wt, 47.0 ± 4.0 vs. Apcs−/−, 41.3 ± 2.4%; P = 0.05) and 20 min (wt, 55.7 ± 3.1 vs. Apcs−/−, 44.4 ± 2.3%; P = 0.01) after incubation with A. fumigatus conidia (Fig. 2g). Hence, SAP basal levels would be sufficient to affect conidia phagocytosis. Murine SAP pre-opsonisation rescued the defect (1 min, P = 0.002; 20 min, P = 0.03) and potentiated phagocytosis by wt neutrophils (1 min, P < 0.0001; 20 min, P = 0.03) (Fig. 2g). Similar results were obtained when human native SAP was used (Supplementary Fig. 7). In addition, membrane expression of CD11b in wt neutrophils was reduced from 97.2 ± 0.4% to 52.5 ± 3.8% and 47.4 ± 2.8%, respectively, at 1 and 20 min after exposure to A. fumigatus (Fig. 2h). In neutrophils from Apcs−/− mice, the decrease in CD11b expression was minor (basal, 97.0 ± 0.5%; 1 min, 59.6 ± 2.4%, P = 0.07; 20 min 57.7 ± 2.2%, P = 0.004), as a consequence of an accumulation of CD11b in the phagocytic cup associated with phagocytosis3,50,51. Murine SAP pre-opsonisation restored CD11b internalization and increased it in wt (Fig. 2h). Therefore, SAP acts as an opsonin for early disposal of A. fumigatus by neutrophils, enhancing phagocytic activity.
The classical and lectin pathways are main initiators of complement activation against A. fumigatus52. Experiments of opsonophagocytosis were therefore performed in the presence of human or mouse sera deficient for different complement components to define the molecular mechanism responsible for SAP-mediated resistance to A. fumigatus. Phagocytosis by human neutrophils was abolished in serum depleted for C3 (−72.0 ± 4.7%, P < 0.0001), C1q (−85.3 ± 0.7%, P < 0.0001) and MBL (−91.7 ± 1.9%, P < 0.0001) compared to normal serum, thus indicating importance of both the classical and the MBL-mediated lectin pathway in resistance against this fungus. In two independent experiments using 5 and 10% of sera, opsonisation with human native SAP potentiated phagocytosis in normal (serum 5%, % of increase, 37.4 ± 10.3%, P = 0.009; serum 10%, 18.3 ± 2.0%, P = 0.01) and MBL-depleted serum (5%, 55.5 ± 17.0%; 10%, 19.8 ± 6.1%, P = 0.04), but not in sera depleted for C3 and C1q (Fig. 3a). Similarly, as assessed in independent experiments, neutrophil phagocytosis was reduced in whole blood of C3−/− (−43.4 ± 10.8%, P = 0.02), C1q−/− (−47.1 ± 11.4%, P = 0.02) and Mbl1/2−/− (−40.2 ± 12.3%, P = 0.01) mice compared to wt, but not in Fb−/− mice (Fig. 3b). Pre-opsonization with murine SAP potentiated phagocytosis in wt [% of increase, 30.8 ± 5.4% (Fig. 3b, left); 94.3 ± 48.2%, P = 0.004 (Fig. 3b, middle); 134.7 ± 45.2%, P = 0.02 (Fig. 3b, right)], Mbl1/2−/− (68.6 ± 28.3%, P = 0.002) and Fb−/− (196.6 ± 61.8%, P = 0.005)] but not in C3−/− and C1q−/− mice (Fig. 3b). These results indicate that the interaction with the classical complement pathway is required for the initiation of SAP-mediated phagocytosis, with no relevance of the alternative pathway. In agreement, a decreased C1q deposition on A. fumigatus conidia was observed after incubation with plasma from Apcs−/− mice (P = 0.05) (Fig. 3c). Moreover, while MBL in serum plays a role in opsonisation, MBL and ficolins, the latter ascertained using specific antibodies (Supplementary Fig. 8), are dispensable for SAP-mediated enhanced phagocytosis.
It was important to assess whether the opsonic activity of SAP was dependent on FcγR or IgG. SAP-mediated opsonophagocytosis did not involve engagement of FcγRs, as ascertained in phagocytosis experiments with specific blocking antibodies (Fig. 3d). As control51, PTX3-mediated phagocytosis was inhibited by FcγRII blockage (Fig. 3d). Depletion of IgGs from the plasma of healthy donors (n = 5) (Fig. 3e) resulted in decreased neutrophil phagocytosis at the 20 min time-point (−26.6 ± 3.3%, P = 0.0006) compared to control plasma. A similar reduction was observed when plasma depleted from SAP (Fig. 3e) was used (1 min, −1.65 ± 13.7%, P = 0.28; 20 min, −20.5 ± 5.9%, P = 0.04). Human SAP pre-opsonization rescued and potentiated neutrophil phagocytosis in IgG-depleted (% of increase, 1 min, 59.6 ± 19.5%, P = 0.04; 20 min, 95.6 ± 14.6%, P = 0.0007), SAP-depleted (1 min, 84.1 ± 26.9%, P = 0.008; 20 min, 127.1 ± 34.1%, P = 0.02) and normal (1 min, 92.0 ± 24.7%; 20 min, 87.9 ± 17.9%, P = 0.008) plasma (Fig. 3f), thus indicating that SAP-mediated opsonophagocytosis did not involve an antibody-mediated engagement of FcγR.
SAP induced complement activation on A. fumigatus culminating in the formation of the membrane attachment complex (MAC; C5b-C9). In plasma of Apcs−/− mice, decreased C3 deposition was observed on A. fumigatus conidia in vitro (Fig. 4a). Pre-opsonisation of A. fumigatus with recombinant murine SAP rescued and increased C3 deposition in Apcs−/− and wt plasma, respectively (Fig. 4a). In the same experiment, a decreased C5a formation was observed in plasma of Apcs−/− compared to wt mice (1 min, 8.5 ± 2.1 vs. 13.0 ± 4.5 ng/ml; 5 min, 14.2 ± 4.3 vs. 35.7 ± 6.9 ng/ml, P = 0.008; 10 min, 26.5 ± 2.2 vs. 53.2 ± 7.5 ng/ml, P = 0.003; 20 min, 61.5 ± 7.8 vs. 113.8 ± 15.5 ng/ml, P = 0.005, respectively, Apcs−/− vs. wt) (Fig. 4b). This defect was rescued by murine SAP opsonisation (1 min, P = 0.003; 5 min, P = 0.007; 10 min, P = 0.002; 20 min, P = 0.04), which also increased C5a in wt plasma (Fig. 4b). Assembly of MAC on the surface of A. fumigatus conidia was decreased in sera depleted for C3 (1 min, −72.2 ± 4.8%; 30 min, −73.0 ± 10.1%; Mean ± SD; P < 0.0001), C1q (1 min, −81.8 ± 3.6%; 30 min, −68.5 ± 4.0%; P < 0.0001), MBL (1 min, −92.2 ± 2.8%, P < 0.001; 30 min, −84.5 ± 3.0%, P < 0.0001) and FB (1 min, −80.6 ± 5.2%; 30 min, −46.4 ± 1.0%; P < 0.0001) and increased in FH-depleted serum (1 min, +5.6 ± 15.8%; 30 min, +55.0 ± 24.9%, P < 0.005). Pre-opsonisation with human SAP enhanced MAC formation on conidia both at 1 min (P = 0.001) and at 30 min (P = 0.008) in the presence of normal serum and in those depleted of MBL, FB, and FH, but not in serum depleted for C1q (Fig. 4c). As assessed in a resazurin-based cell viability assay, pre-opsonisation of human SAP resulted in increased microbicidal activity of A. fumigatus conidia in normal serum and in serum depleted of MBL (Fig. 4d). The effect was abolished in human sera depleted of C3 or C1q. Posaconazole (POC) was used as antifungal control (Fig. 4d). Actual fungal killing was also ascertained as CFU counts (Supplementary Fig. 9). SAP-mediated complement activation on A. fumigatus was antibody-independent. Decreased C3 deposition was observed in IgG-depleted plasma, both at 1 (−53.5 ± 7.9%, P = 0.02) and 20 min (−74.2 ± 4.7%, P = 0.002) compared to control. In SAP-depleted plasma, reduction of C3 deposition corresponded to −30.5 ± 9.1%, (1 min, P = 0.025) and −58.6 ± 4.9%, (20 min, P < 0.0001). Pre-opsonisation with human SAP rescued and increased C3 deposition in both IgG- (% of increase, 1 min, 87.9 ± 25.2%, P = 0.022; 20 min, 139.6 ± 14.6%, P < 0.0001) and SAP-depleted (1 min, 14.3 ± 4.4%; 20 min, 144.5 ± 23.8%, P = 0.001) plasma as well as in the control (1 min, 75.0 ± 43.4%; 20 min, 36.1 ± 13.3%, P = 0.008) (Fig. 3g), thus suggesting an effect of SAP in promoting complement activation on A. fumigatus under mimed immunosuppressive conditions of antibody deficiencies.
Having found that SAP plays a non-redundant role in resistance to A. fumigatus and that neutrophils were involved, it was important to explore the role and significance of other phagocytic cell populations using in vitro, ex vivo and in vivo approaches. As shown in Fig. 5a, in a whole blood assay, in vitro SAP increased the phagocytic activity of neutrophils [% of increase, wt, 134.3 ± 19.6%, P < 0.0001 (1 min); 103.8 ± 25.4%, P = 0.003 (20 min); Apcs−/−, 176.3 ± 34.2%, P = 0.0004 (1 min); 110.3 ± 311.0%, P < 0.0001 (20 min)] and monocytes [wt, 138.2 ± 12.7%, P < 0.0001 (1 min); 83.1 ± 14.5%, P = 0.002 (20 min); Apcs−/−, 104.4 ± 20.9%, P = 0.005 (1 min); 148.3 ± 23.7%, P < 0.0001 (20 min)], but not of DC (Fig. 5a). Similar results were obtained with human myelomonocytic cells in vitro (30 min; P = 0.03) (Fig. 5b).
Bone marrow-derived myelomonocytic cells were used to further investigate the impact of SAP on this compartment. In the presence of correspondent serum (10%), bone marrow-derived macrophages obtained from Apcs−/− mice produced decreased levels of CXCL1 (Apcs−/−, 7.5 ± 2.0 vs. wt, 8.6 ± 1.1 ng/ml), CXCL2 (Apcs−/−, 15.6 ± 7.2 vs. wt, 29.8 ± 3.7 ng/ml), TNF-α (Apcs−/−, 3.7 ± 0.6 vs. wt, 4.5 ± 0.4 ng/ml), IL-6 (Apcs−/−, 3.1 ± 0.8 vs. wt, 4.7 ± 0.9 ng/ml) after stimulation with A. fumigatus conidia (24 h) (Fig. 5c).
We next investigated the impact of the Apcs deficiency on macrophage and DC function in vivo. As assessed by FACS, injection of conidia pre-opsonized with murine SAP increased phagocytosis (4 h) in BALF macrophages (% of increase, wt, 41.8 ± 3.4%, P = 0.004; Apcs−/−, 26.8 ± 5.0%, P = 0.03) and neutrophils (wt, 81.5 ± 32.6%, P = 0.03; Apcs−/−, 285.9 ± 60.2%, P = 0.0003) derived from wt and Apcs−/− mice, but not in DCs (Fig. 5d). SAP-deficiency did not affect phagocytosis in BALF macrophages (51.4 ± 4.9 vs. 51.0 ± 14.1%; Apcs−/ vs. wt) and DCs (7.8 ± 1.5 vs. 11.2 ± 3.4%; P = 0.18), but reduced it in neutrophils (8.1 ± 2.5 vs. 16.0 ± 3.7%, Apcs−/ vs. wt, % of decrease: −49.2 ± 15.5%; P = 0.05) (Fig. 5d). In conclusion, although SAP facilitates recognition, phagocytosis and functional responses by macrophages, the predominant effector cells affected by Apcs deficiency in vivo are neutrophils.
Collectively, these results indicate that SAP-mediated resistance against A. fumigatus involves complement-mediated opsonization, neutrophil phagocytosis and pathogen killing.
Therapeutic potential of SAP
Having established that SAP is an essential component of resistance to A. fumigatus, it was important to obtain indications as to its therapeutic potential. A. fumigatus infection is a clinical challenge in patients with congenital or acquired immune deficiency. The most important risk factor for IPA is represented by neutropenia and monocytopenia that occur in immune-compromised patients40. The potential therapeutic effect of SAP was determined in transiently myelosuppressed mice. Therefore, we used a model of cyclophosphamide-induced immune suppression to obtain indications as to the therapeutic potential of SAP. Dosage of A. fumigatus conidia was newly optimized in mice after 2-day treatment with cyclophosphamide (150 mg/kg). Human native SAP (4 mg/kg) was injected intraperitoneally (i.p.) at 2 and 24 h after infection. Immune-compromised mice did not survive infection with 5 × 107 (17/17; MST 4 days), 1 × 107 (7/7; MST 4 days) and 5 × 106 (8/8; MST 5 days) conidia (Fig. 6a). SAP protected immune-compromised mice increasing survival to 20% (5 × 107; 12/15, MST 4; P = 0.002), 62.5% (1 × 107; 3/8, MST > 10; P = 0.001) and 80% (5 × 106; 2/10, MST > 10; P = 0.004), respectively (Fig. 6a). A 12-fold reduction in fungal burden was observed in the lungs of SAP-treated mice 16 h after infection with 1 × 107 conidia (median 2.0 × 106 CFU, IQR 9.0 × 106−8.0 × 106 vs. 4.4 × 107 CFU, IQR 9.1 × 107−7.5 × 107; P = 0.02) (Fig. 6b). Similarly, SAP (50 µg/mouse) administered i.t. 2 h after infection (5×106 conidia) protected immune-compromised mice. 2/13 untreated mice survived (15.4%; MST 7 days), whereas 9/12 SAP-treated mice survived (75.0%; MST > 10; P = 0.003) (Fig. 6c). I.t. SAP treatment resulted in a 23-fold reduction of lung CFU 16 h after infection (median 2.3 × 104, IQR 1.1 × 105−1.0 × 105 vs. 5.3 × 106, IQR 9.5 × 106−7.7 × 106; P = 0.008) (Fig. 6d).
Genetic variation in human SAP influences the risk of IPA
In view of the critical role of SAP during fungal infection in vivo, we next investigated the relationship between genetic variation in the human APCS gene and susceptibility to IPA in patients at-risk. For such, we assessed the cumulative incidence of IPA in patients from the IFIGEN cohort undergoing allogeneic hematopoietic stem-cell transplantation (Supplementary Table 2) according to recipient or donor APCS genotypes of the single nucleotide polymorphisms (SNPs) rs2808661 and rs3753869 (Table 1). These SNPs were selected based on their ability to tag surrounding variants with a pairwise correlation coefficient r2 of at least 0.80 and a minor allele frequency ≥5%, covering therefore the whole genetic variability across the APCS locus (Supplementary Fig. 10).
The rs2808661 SNP in the donor genome was associated with an increased risk of IPA after transplantation, whereas recipient rs2808661 displayed a trend towards increased risk (Fig. 7a). The cumulative incidence of IPA for donor rs2808661 was 23% for GG, 21% for GA (P = 0.67) and 56% for AA genotypes (P = 0.04), whereas for recipient rs2808661, the cumulative incidence ranged from 20% for GG to 25% for GA (P = 0.58) and 36% for AA (P = 0.08) (Fig. 7a). Upon modeling a dominant mode of inheritance, the key contribution of the donor AA genotype to the risk of infection was confirmed. As for the rs3753869 SNP, association with risk was only detected in donor genomes (Fig. 7b). The cumulative incidence of IPA was 21% for CC, 26% for CA (P = 0.30) and 43% for AA genotypes (P = 0.03), respectively.
In a multivariate model accounting for age, gender, type of transplantation, acute graft-versus-host disease (GVHD) and antifungal prophylaxis, the AA genotype in rs3753869 conferred a 3.6-fold increased risk of developing IPA (Table 2). Collectively, these results highlight genetic variation at the APCS locus as a novel risk factor regulating susceptibility to IPA.
To assess whether genetic variation at the APCS locus was associated with different SAP levels, this pentraxin was measured in samples from hematological patients from the FUNBIOMICS cohort53. SAP serum levels were similar in IPA patients (18.6 ± 1.2 µg/ml, n = 20) compared to controls (16.6 ± 1.3 µg/ml, n = 14; P = 0.2), whereas higher amounts were present in BALFs (65.7 ± 26.4 ng/ml, n = 9; vs. 2.6 ± 0.7 ng/ml, n = 7; P = 0.0007) (Fig. 7c). Once analyzed for the single donor genotype, SAP concentration in sera was to 18.8 ± 1.7 µg/ml for GG (n = 12), 23.7 ± 0.5 µg/ml for GA (n = 3) and 14.7 ± 0.9 µg/ml for AA (n = 4; P = 0.103, AA vs. GG; P = 0.0006, AA vs. GA; P = 0.05, AA vs. GG+GA) in donor rs2808661 (Fig. 7d). Regarding donor rs3753869, SAP concentration was 19.1 ± 1.3 µg/ml for CC (n = 13), 23.17 ± 2 .7 µg/ml for CA (n = 3) and 13.3 ± 2.9 µg/ml for AA (n = 4; P = 0.03, AA vs. CC; P = 0.06, AA vs. CA; P = 0.02, AA vs. CC+CA) (Fig. 7e). No differences were observed for GG vs. GA and CC vs. CA, respectively, in recipient rs2808661 and rs3753869 (Supplementary Fig. 11). Therefore, the increased risk of IPA according to donor APCS rs2808661 and rs3753869 genotypes is associated with lower serum levels of SAP.
Discussion
The results presented here show that SAP is an essential component of resistance against the fungal pathogen A. fumigatus. Apcs−/− mice showed increased susceptibility to infection by A. fumigatus, a phenotype rescued by in vitro opsonization of fungi or in vivo administration of murine SAP. In vitro and in vivo observations suggest that complement activation following fungal recognition and phagocytosis of opsonized fungi are responsible of SAP-mediated resistance.
SAP has been shown to bind a variety of microbial species and to have opsonic activity16,19,22,23,24,26, though under selected conditions, inhibition of phagocytosis has been reported26,54. Recently, SAP was found to bind amyloid fibrils produced on the surface of Candida albicans and to dampen recognition by the innate immune system55. Here, we found that SAP binds A. fumigatus conidia and facilitates phagocytosis, and evidence suggests that this pathway underlies the role of this fluid phase PRM in resistance to this fungus.
The role of SAP in resistance to A. fumigatus is reminiscent of findings with the related molecule PTX338. Genetic evidence in mice2,38 and humans39,40,41,42,43,44,45,46,47 suggests that PTX3 plays an essential role in resistance against selected pathogens, in particular A. fumigatus. SAP is produced in the liver whereas PTX3 is stored in neutrophil granules and produced by macrophages as well as by other cell types1,2. It is therefore likely that SAP and PTX3 exert through different molecular mechanisms51 complementary functions systemically and in peripheral tissues in antifungal resistance.
In hematological patients, we found that BALF had substantially higher levels of SAP in IPA compared to controls, but not peripheral blood. Moreover, in patients undergoing stem cell transplantation, donor genetic variation was associated with increased susceptibility to IPA. These results raise the interesting possibility that a relevant source of SAP is represented by hematopoietic progenitor cells. The liver is the major dominant source of SAP7,9,10. However, local expression of SAP has been reported in atherosclerosis56 and in fibrotic lesions10. By bioinformatics analysis, we found evidence of APCS gene expression in human myelomonocytic progenitors, myeloid cells in acute coronary syndromes and in sepsis and myeloid murine cells (Supplementary Fig. 12a–f). Moreover, APCS was expressed in monocytes in our own cohort of COVID-19 patients57 as assessed by RNAseq (Supplementary Fig. 12f). Further studies are required to define the relevant extrahepatic cellular source of SAP in patients with IPA.
There are well-known risk factors that predispose to fungal disease, particularly in immunocompromised and severely ill patients, but these alone fail to convincingly explain the development of infection in all patients. Several studies have reported an expanding number of SNPs associated with IPA40,43,58. We now demonstrate that SNPs in APCS also contribute to IPA via molecular mechanisms influencing opsonisation and activation of the complement cascade in response to fungal infection. Although genetic variation in APCS has been evaluated in the context of other diseases, including type 2 diabetes and polyneuroendopathic amyloidosis59, these are not acknowledged as relevant conditions predisposing to IPA. Our analyses have indeed confirmed a role for APCS SNPs in IPA that is independent from clinical risk factors often associated with the infection in the stem-cell transplantation setting, such as the development of severe acute GVHD. These data may become relevant in the clinical management of patients at-risk of IPA, since the identification of patients that may benefit the most from SAP-based therapies may be possible.
The frequency of APCS homozygous mutant genotypes associated with IPA is relatively low in the population. However, this fact further strengthens the critical role of these genetic variants as risk factors for IPA, since carriers of these genotypes reach cumulative incidence values of infection around 50%. Therefore, although rare in the general population, specific APCS genotypes are greatly enriched among IPA patients, suggesting a particularly relevant mechanism in the pathogenesis of infection, thus supporting a potential clinical applicability for screening donors, for patient management, e.g., to direct antifungal prophylaxis or to intensify fungal diagnostics in patients at higher genetic risk.
SAP has been shown to regulate matrix deposition by regulating macrophages, which play a key role as orchestrators of tissue remodeling10,31,32,33. These preclinical results paved the way to clinical development of SAP (PRM-151) as an anti-fibrotic agent34,35. Early Phase II results of PRM-151 in idiopathic pulmonary fibrosis are encouraging36,37. Current therapeutic limitations as well as concerns over the emergence of antifungal resistance are inspiring the search for novel host-directed therapies. The results reported here suggest that SAP may be explored as a therapeutic agent in Aspergillus infections, which represent a formidable clinical challenge.
Methods
Animals
Wild-type (wt) C57BL/6J and C57BL/6NJ mice between 8 and 10 weeks of age were, respectively, purchased from Charles River Laboratories (Calco, Como, Italy) and from The Jackson Lab (Bar Harbor ME, US). Apcs−/− mice were generated as described27. C1q−/− mice were kindly provided by Prof. John Lambris (University of Pennsylvania, USA). C3−/−, Mbl1/2−/− and Fb−/− mice were purchased from Jackson Lab (Bar Harbor ME, US). Apcs−/−, C1q−/−, C3−/−, Mbl1/2−/− mice were on a C57BL/6J genetic background, whereas Fb−/− mice were on C57BL6/NJ. wt littermates were used as control for Mbl1/2−/−, C1q−/− and Fb−/− mice. Co-housed wt were used as control for C3−/−mice. Mice were housed and bred in the SPF animal facility of Humanitas Clinical and Research Center in individually ventilated cages (light-dark cycle: from 7 a.m. to 7 p.m.; temperature and humidity: 20–24 °C and 40–60%). Procedures involving animals and their care were conformed to protocols approved by the Humanitas Clinical and Research Center (Rozzano, Milan, Italy) in compliance with national (4D.L. N.116, G.U., suppl. 40, 18-2-1992) and international law and policies (EEC Council Directive 2010/63/EU, OJ L 276/33, 22-09-2010; National Institutes of Health Guide for the Care and Use of Laboratory Animals, US National Research Council, 2011). The study was approved by the Italian Ministry of Health (approval n. 71/2012-B, issued on the 09/03/2012, 44/2015-PR issued 28/01/2015 and 742/2016-PR issued on the 26/07/2016). All efforts were made to minimize the number of animals used and their suffering.
Population cohorts
For the genetic association study, a total of 483 hematological patients of European ancestry undergoing allogeneic HSCT at Instituto Português de Oncologia, Porto, and at Hospital de Santa Maria, Lisbon (Portugal), were enrolled in the IFIGEN study between 2009 and 2015. The demographic and clinical characteristics of the patients are summarized in Supplementary Table 1. One hundred-eleven cases of probable/proven IPA were identified according to the 2008 criteria from the European Organization for Research and Treatment of Cancer/Mycology Study Group (EORTC/MSG)60. Twenty-one patients were excluded from the study based on the “possible” classification of infection. The levels of SAP were measured in clinical specimens from adult hematological patients enrolled in the FUNBIOMICS study53 at the Leuven University Hospitals, Leuven (Belgium). Cases of probable/proven IPA were diagnosed as above60. The control group included patients with no evidence for the presence of Aspergillus spp. in the bronchoalveolar lavage (BALF) sample (negative culture, negative microscopy, and a galactomannan optical density index <0.5). Patients with “possible” disease were excluded from the study and no mold-active antifungals were administered by the treating physician(s) before sample collection. BALF and serum samples were collected using standard protocols, as described53.
Ethics statement
Approval for the IFIGEN study was obtained from the Ethics Committee for Research in Life and Health Sciences (CEICVS) of the University of Minho, Portugal (no. 125/014), the Ethics Committee for Health of the Instituto Português de Oncologia - Porto, Portugal (no. 26/015), the Ethics Committee of the Lisbon Academic Medical Center, Portugal (no. 632/014), and the National Commission for the Protection of Data, Portugal (no. 1950/015). The FUNBIOMICS study was approved by CEICVS and the Ethics Committee of the University Hospitals of Leuven, Belgium (no.126/014). Written informed consent was obtained from the patient or a representative prior to collection of samples.
The study on COVID-19 patients was approved by the local Humanitas Clinical and Research Center Ethical Committee (authorization 233/20). This study was conducted as described57 on a cohort of patients admitted to Humanitas Clinical and Research Center (Rozzano, Milan, Italy) with a laboratory-confirmed diagnosis of COVID-19.
In healthy donors, neutrophils and monocytes were isolated from peripheral blood upon approval by the Humanitas Research Hospital Ethical Committee (approval obtained on January 28th, 2016).
Invasive pulmonary aspergillosis
A clinical strain of A. fumigatus was isolated from a patient with a fatal case of pulmonary aspergillosis was kindly provided by Dr. Giovanni Salvatori (Sigma-tau, Rome, Italy). A. flavus (#MCV-C#1; ATCC® 204304) and A. terreus (Thom, #MF4833; ATCC® 20541) were purchased from ATCC®. The growth and culture conditions of A. fumigatus, A. flavus and A. terreus conidia were as described38. The Candida albicans strain CAF3-1 was kindly provided by Dr. Marina Vai (Dept. of Biotechnology and Biosciences, University of Milano-Bicocca). C. albicans blastospores were grown at 25 °C in rich medium [YEPD (yeast extract, peptone, dextrose), 1% (w/v) yeast extract, 2% (w/v) Bacto Peptone, and 2% (w/v) glucose] supplemented with uridine (50 mg/l) and the hyphae induced as described61. For intratracheal (i.t.) injection, mice were anesthetized with ketamine (100 mg/kg; i.p.) and xylazine (10 mg/kg i.p.). After surgical exposure, a volume of 50 μl PBS2+, pH 7.4, containing 1 × 108 or 5 × 107 resting conidia (>95% viable, as determined by serial dilution and plating of the inoculum on Sabouraud dextrose agar) were delivered into trachea under direct vision using a catheter connected to the outlet of a micro-syringe (Terumo, Belgium). Survival to infection was daily monitored for 10d later. C. albicans blastospores (5 × 106 in a final volume of 50 μl PBS) were intravenously (i.v.) injected and survival monitored for 20 days. Dying mice were euthanized after evaluation of the following clinical parameters: body temperature dropping, intermittent respiration, solitude presence, sphere posture, fur erection, non-responsive alertness, and inability to ascent when induced.
In experiments of in vivo phagocytosis, mice were i.t. injected with 5 × 107 heat-inactivated fluorescein isothiocynate (FITC)-labeled conidia and euthanized 4 h later. In rescue experiments, conidia (1 × 109) were opsonized with murine recombinant SAP (50 µg/ml; lot. #MPX1518061; R&D Systems) for 1 h at r.t. in PBS, pH 7.4, containing 0.01% (vol/vol) Tween-20® (Merck-Millipore). After washing of unbound protein, a volume of 50 µl (5 × 107 conidia) was i.t. injected. Alternatively, a single i.v. injection of murine SAP (50 µg/mouse; lot. #MPX1518061; R&D Systems) previously dialyzed in PBS (pH 7.4) in order to eliminate sodium azide was given after 2 h i.t. injection of conidia (5 × 107). In therapy experiments, immunosuppression was induced by i.p. injection of 150 mg/kg cyclophosphamide (150 µl per mouse of 20 mg/ml solution) 2 days before infection and indicated doses of conidia were i.t. injected. Native human SAP (lot. #2787319; Merck-Millipore) was dialyzed in PBS (pH 7.4) and i.p. injected at the dose of 4 mg/kg at 2 and 24 h after infection. In experiments of local therapy, 50 µg human SAP (1 mg/ml; 50 µl in PBS) were i.t. administred 2 h after infection.
BALFs collection and analysis
BALFs were performed with 1.5 ml PBS, pH 7.4, containing protease inhibitors (Complete tablets, Roche Diagnostic; PMSF, Sigma-Aldrich/Merck) and 10 mM EDTA (Sigma-Aldrich/Merck) with a 22-gauge venous catheter. BALFs were centrifuged, and supernatants were collected for quantification of cytokines as described below. After erythrocyte lysis with ACK solution (pH 7.2; NH4Cl 0.15 M, KHCO3 10 mM, EDTA 0.1 mM), cells were resuspended in PBS, pH 7.4, containing 10 mM EDTA and 1% heat-inactivated fetal bovine serum (FCS; Sigma-Aldrich/Merck), stained with LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit (ThermoFisher Scientific-Molecular Probes) or Zombie Aqua™ Fixable Viability Kit (Biolegend, US), and analyzed as reported in Supplementary Fig. 13 by BD FACS LSRFortessaTM Flow Cytometer (BD Biosciences) and BD FACSDivaTM Software (version 6.2) with the following specific antibodies: peridinin chlorophyll protein complex (PerCP)- or brilliant violet (BV) 650-labeled anti-CD45 (#30-F11, IgG2B; used at 4 µg/ml; Cat. No. 103130, Biolegend, and Cat. No. 563410, BD Biosciences, respectively); FITC- or phycoerythrin (PE)-CF594-labeled anti-Ly6G (#1A8, IgG2A; used at 4 µg/ml; Cat. No. 11-9668-82, eBioscience-Invitrogen, and Cat. No. 562700, BD Biosciences, respectively); allophycocyanin (APC)-cyanine (Cy)7- or BV421-labeled anti-CD11b (#M1/70, RUO, IgG2B; used at 1 µg/ml; Cat. No. 557657 and Cat. No. 562605, BD Biosciences); BV421-labeled anti-Ly6C (#AL-21, RUO, IgM; used at 1.6 µg/ml; Cat. No. 562727, BD Biosciences), APC-labeled anti-CD11c (#HL3, IgG1; used at 1.6 µg/ml; Cat. No. 550261, BD Biosciences) and PE-Cy7-labeled anti-F4/80 (#BM8, IgG2A; used at 1.6 µg/ml; Cat. No. 123110, Biolegend). At least 10,000 events of CD45+ cells were considered as cut-off for the the FACS analysis.
Lung homogenates and analysis
Lungs were removed 16 h after infection and homogenized in 1 ml PBS, pH 7.4, containing 0.01% (vol/vol) Tween-20® (Merck-Millipore) and protease inhibitors. Samples were serially diluted 1:10 in PBS and plated on Sabouraud dextrose agar for blinded CFU counting. For lysate preparation, lungs were collected at 4 h and homogenized in 50 mM Tris-HCl, pH 7.5, containing 2 mM EGTA, 1 mM PMSF, 1% Triton X-100 (all from Sigma-Aldrich/Merck), and complete protease inhibitor cocktail. Total proteins were measured by DC Protein Assay, according to manufacturer’s instructions (Bio-Rad Laboratories). Western blot analysis for C3 was performed after loading 10 µg of lung protein extracts on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The goat polyclonal anti-C3 (diluted 1:3000; Merck-Millipore, Cat. No. 204869) and HRP-conjugated donkey anti-goat IgG (diluted 1:5000; R&D Systems, Cat. No. HAF-109) were used. The monoclonal anti-vinculin (#hVIN-1; used at 0.5 µg/ml; Sigma-Aldrich/Merck, Cat. No. V9264) was used as loading control. C3d bands were quantified by Fiji-ImageJ (NIH, Bethesda USA; version 2.1.0/1.53.c) as a ratio of mean gray intensity values of each protein relative to vinculin bands.
Cells and in vitro phagocytic activity
Phagocytosis assay in whole blood of A. fumigatus conidia by mouse and human neutrophils was performed as described51. Briefly, conidia (1 × 109) were labeled (1 h, r.t.) with FITC (Sigma-Aldrich/Merck; 5 mg/ml in DMSO), and eventually opsonized (1 h, r.t) with murine or human SAP (100 µg/ml; 1.1 µM) and PTX3 (50 µg/ml; 1.1 µM). An amount of 1 × 107 FITC-conidia were added to 200 µl of mouse whole blood (collected with heparin from cava vein) and incubated for 1 and 20 min at 37 °C in an orbital shaker. Samples were immediately placed on ice and ACK lysis solution was added to lyse erythrocytes. Murine neutrophils, monocytes, macrophages and dendritic cells were analyzed by BD FACS LSRFortessaTM Flow Cytometer (BD Biosciences) as above described and indicated in Supplementary Fig. 13, and frequency and/or mean fluorescence intensity (MFI) of FITC+ neutrophils and/or CD11b expression were reported.
Human neutrophils were isolated from fresh whole blood of healthy volunteers through separation from erythrocytes by 3% dextran (GE Healthcare Life Sciences) density gradient sedimentation followed by Ficoll-Paque PLUS (GE Healthcare Life Sciences) and 62% Percoll® (Sigma-Aldrich/Merck) centrifugation as previously described51. Blood monocytes were obtained by Ficoll-Paque PLUS (GE Healthcare Life Sciences) and 46% Percoll® (Sigma-Aldrich/Merck). Purity, determined by FACS analysis on forward scatter/side scatter parameters, was routinely > 98%. 1 × 105 cells were incubated for 1 and 30 min at 37 °C in 50 µl RPMI-1640 medium with 5 and/or 10% normal human serum (NS) or complement depleted sera, optionally with autologous human plasma-heparin, and 1 × 106 FITC-labeled A. fumigatus conidia. When indicated, cells were resuspended in 10% normal plasma-heparin from healthy donors depleted from IgGs or SAP as described below. Cells were transferred on ice and, after washing with PBS, pH 7.4, containing 10 mM EDTA and 0.2% bovine serum albumin (BSA; Sigma-Aldrich/Merck), FITC fluorescence in neutrophils (defined as FSC-Ahigh/SSC-Ahigh) and monocytes (defined as FSC-Adim/SSC-Adim) was analyzed by BD FACS CantoTM II Flow Cytometer (BD Biosciences) and BD FACSDivaTM Software (version 6.1.1). NS and sera depleted of C3, C1q, MBL, FB and FH were all obtained from CompTech (Complement Technology, Inc., USA). In blocking experiments, neutrophils were-preincubated for 1 h on ice in the presence of following mouse IgG1 (used at 10 µg/ml/1 × 106 cells): anti-CD16 (FcγRIII) (#3G8; BioLegend, Cat. No. 302002); anti-CD32 (FcγRII) (#FUN-2; BioLegend, Cat. No. 303202), or anti-CD32 (FcγRII) (#AT10; Merck-Millipore, Cat. No. MABF925); anti-CD64 (FcγRI) (#10.1; eBioscience-ThermoFisher, Cat. No. 14-0649-82); anti-M-ficolin (#036-051 1; Santa Cruz Biotechnology, Cat. No. sc-80486); L-ficolin (#FCN219; Santa Cruz Biotechnology, Cat. No. sc-80484); irrelevant mouse IgG1 (#MG1-45, BioLegend, Cat. No. 401402, or #P3.6.2.8.1, ThermoFisher Scientific, Cat. No. 16-4714-82). All these antibodies were previously dialyzed in PBS, pH 7.4.
Bone marrow-derived (BM) cells were isolated from femurs and tibiae and cultured at density of 1.5 × 106 cells/ml in RPMI-1640 medium supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS; Sigma-Aldrich/Merck), 2mM l-Glutammine, 1% Pen/Strept for 24 h. Non-adherent cells were then harvested and cultured with 20 ng/ml of murine recombinant M-CSF (PeproTech) to obtain macrophages. Cells were washed and medium replaced at day 3. BM macrophages (1 × 106 cells/ml) were stimulated on day 6 with heat-inactivated A. fumigatus conidia (ratio 1:5, cell:conidia) or 10 ng/ml LPS (Sigma-Aldrich/Merck) in the presence of 10% freshly isolated serum obtained from wt and Apcs−/− mice to preserve functions of complement. Cell supernatants were collected (24 h) for cytokine measurement.
Complement deposition on Aspergillus fumigatus
A volume of 10 µl PBS, pH 7.4, containing 1 × 107 conidia eventually opsonized with murine SAP (100 µg/ml per 1 × 109 conidia, 1 h at r.t.) was incubated (37 °C) in round bottom wells of Corning® 96-well polypropylene microplates for the indicated time points with 20 µl mouse plasma-heparin or 20 µl human NS, complement depleted sera (diluted in PBS at 10% and 30%). When indicated, plasma-heparin obtained from healthy donors depleted of IgGs or SAP (diluted in PBS at 10%) was used. Complement deposition was blocked by addition of EDTA (10 mM final concentration) and by cooling in ice. After centrifugation (1450 x g, 10 min at 4 °C), when indicated, supernatant was collected for mouse C5a measurement by ELISA. Conidia were washed and incubated (1 h, at 4 °C) with PBS, pH 7.4, 2 mM EDTA, 1% BSA containing the following primary antibodies: goat anti-human/mouse C3 and activation fragments (diluted 1:5000; Merck-Millipore, Cat. No. 204869); rat anti-mouse C1q (IgG1, #7H8; used at 1 µg/ml; HyCult® Biotech, Netherlands, Cat. No. HM1044); rabbit anti-human C5b-C9 (MAC) (diluted 1:2000; Complement Technology, Inc., Cat. No. A227); or correspondent irrelevant IgGs. Conidia were then incubated (1 h, at 4 °C) with Alexa Fluor® 488 and 647-conjugated species-specific cross-adsorbed detection antibodies (used at 2 µg/ml; ThermoFisher Scientific-Molecular Probes, Cat. No. A-11055, A-48272, A-21206) and analyzed by BD FACS CantoTM II Flow Cytometer (BD Biosciences) using forward and side scatter parameters to gate at least 8000 conidia. After each step, conidia were extensively washed with PBS, pH 7.4, 2 mM EDTA, 1% BSA. Results were expressed as frequency of conidia showing fluorescence compared with irrelevant controls and as geometric conidia MFI.
Fungal viability assay
Effect of SAP on A. fumigatus killing was evaluated by a resazurin-based cell viability assay. A volume of 10 µl PBS, pH 7.4, containing 1.5 × 105 conidia eventually opsonized with human SAP (100 µg/ml per 1 × 109 conidia, 1 h at r.t.) was placed into sterile round bottom Corning® 96-well polypropylene microplate and incubated for 1 and 30 min at 37 °C with 20 µl of human serum and different complement component-depleted sera (30%). After incubation, plates were immediately cooled on ice and cold-centrifuged (1450 x g, 10 min at 4 °C), and then supernatant removed. Conidia not incubated with serum were used as a negative control. Conidia treated with the fungicide drug Posaconazole (POC; 1 µM) were considered as positive control in the assay. Preparation of AlamarBlueTM Cell Viability Reagent and test was performed according with manufacture’s instructions (ThermoFisher Scientific-Invitrogen). A volume of 100 µl AlamarBlueTM solution (10 µl of AlamarBlueTM reagent and 90 µl of Sabouraud dextrose broth) was added to each well. After 17 h reaction at 37 °C, fluorescence (excitation/emission at ≈530–560/590 nm) intensity was measured by microplate reader SynergyTM H4 (BioTek, France). Results represent ratio of fluorescence intensity values relative to those measured in negative controls. The actual killing of fungi was controlled as CFU count.
Proteins
Recombinant murine SAP from mouse myeloma cell line NSO was used (lot. #MPX1518061; R&D Systems). Native SAP from human serum was purchased by Merck-Millipore (lot. #2787319). Recombinant murine PTX3 was purified from supernatant of Chinese hamster ovary cells (ATCC®-CRL-12023) stably expressing the protein, as described previously51. Purity of the recombinant protein was assessed by SDS-PAGE followed by silver staining. Proteins contained <0.125 endotoxin units/ml as checked by the Limulus amebocyte lysate assay (BioWhittaker®, Inc.). SAP levels were measured in mouse plasma by ELISA (DuoSet ELISA; R&D Systems) after collection of blood from the cava vein. Murine MPO was measured in BALFs by ELISA (DuoSet ELISA; R&D Systems). Murine CXCL1, CXCL2, TNF-α and IL-6 were measured in supernatants by ELISA (DuoSet ELISA; R&D Systems) according with manufacturer’s instructions. Murine C5a was measured either in plasma-heparin or in BALFs previously stored at −80 °C by DuoSet ELISA (R&D Systems) maintaining EDTA (10 mM) throughout the assay in order to stop the activation of the complement cascade. Human SAP was measured in sera and BALFs of patients by ELISA (lot. #P105304, DuoSet ELISA; R&D Systems) according with manufacturer’s instructions.
IgGs and SAP depletion
Plasma-heparin of healthy volunteers was collected in BD Vacutainer® and maintened (1 h) on ice during procedures of depletion to avoid the activation of the complement. IgG depletion was obtained by passing human plasma-heparin (2 ml) on protein-G SepharoseTM Fast Flow (GE Healthcare, Sweden) column, as indicated by manufacturer’s instructions. Depletion of SAP was obtained by passing plasma heparin (2 ml) of each donor through different columns of protein-G SepharoseTM Fast Flow (GE Healthcare, Sweden) previously cross-linked with a purified rabbit polyclonal anti-SAP (1 mg/1 ml of resin) (Merck-Millipore, Cat. No. 565191). Dimethyl pimelimidate (Sigma-Aldrich/Merck) was used as cross-linking reagent. Bound proteins were eluted with 0.1 M Glicine-HCl pH 2.8, concentrated with Vivaspin® 10,000 MWCO (Sartorius, Germany) and titrated with the PierceTM Coomassie (Bradford) protein assay kit (ThermoFisher Scientific). The actual depletion of SAP and IgGs was evaluated by Western blot analysis after loading 1 µl/lane plasma-heparin on SDS-PAGE. Proteins eluted from the anti-SAP column were also loaded to ascertain its capture. The mouse monoclonal anti-SAP (used at 0.5 µg/ml; #910119; R&D Systems, Cat. No. MAB1948) and HRP-conjugated sheep anti-mouse IgG (diluted 1:3000; GE Healtcare, Sweden, Cat. No. NA931V) or a goat anti-human IgG (used at 1 µg/ml; Jackson ImmunoResearch, Cat. No. 109-006-097) and HRP-conjugated anti-goat IgG (diluted 1:5000; R&D Systems, Cat. No. HAF-109) were used to detect, respectively, SAP and IgGs. The different intensity of SAP (or IgG) bands between lanes Donor A–E vs. Donor A–E/IgG depleted (or SAP depleted) are due to steric hindrance of antibody recognition, having SAP and IgG low chains similar molecular weight.
Binding of SAP
Aspergillus conidia (1 × 108 CFU) were cultured 4 and 16 h under static condition in Sabouraud medium to, respectively, allow conidia swelling and germination. C. albicans blastospore, yeast and hypheae (1 × 107 CFU) were obtained as described61. After washing with PBS2+, pH 7.4, containing 0.01% (vol/vol) Tween-20® (Merck-Millipore), resting and swollen conidia or hyphae were incubated (1 h, r.t.) with biotin-labeled murine SAP (R&D Systems) at concentrations ranging from 0.1 to 10 µg/ml in PBS2+, pH 7.4, containing 2% BSA (Sigma-Aldrich/Merck). In competition experiments, human SAP or CRP (50 µg/ml; lot. #B68019; Merck-Millipore) or murine PTX3 (50 µg/ml) were further added. After extensive washing, samples were incubated (30 min, r.t.) with Alexa Fluor® 647-conjugated streptavidin (diluted 1:1000; ThermoFisher Scientific-Molecular Probes, Cat. No. S-32357) and binding was evaluated by FACS as frequency and MFI and visualized by confocal microscopy as described. In some experiments, a rat monoclonal anti-SAP (IgG2A, #300103; used at 5 µg/ml; R&D Systems, Cat. No. MAB2558) and an Alexa Fluor® 647-conjugated goat anti-rat IgG (diluted 1:500; ThermoFisher Scientific-Molecular Probes, Cat. No. A21247) were also used. CRP was cross-linked with BS3 (Bis[sulfosuccinimidyl]suberate; Thermo Fisher Scientific) according to the manufacturer’s instructions.
Microscopy
Five micrometer cryostat sections of mouse lungs injected with 5 × 107 heat inactivated FITC-labeled conidia (4 h) were incubated in 5% of normal donkey (Sigma-Aldrich/Merck) serum, 2% BSA (Sigma-Aldrich/Merck), 0.1% Triton X-100 (Sigma-Aldrich/Merck) in PBS2+, pH 7.4, (blocking buffer) for 1 h at room temperature. Sections were incubated with the following primary antibodies diluted in blocking buffer for 2 h at r.t.: rabbit polyclonal anti-SAP (diluted 1:500; Merck-Millipore, Cat. No. 565191); rat monoclonal Ab anti-C3 (C3b/iC3b/C3d) (IgG2A, #2/11; used at 5 µg/ml; Hycult® Biotech, Cat. No. HM1065) previously conjugated with Alexa Fluor® 647 through the Antibody Labeling kit (ThermoFisher Scientific-Molecular Probes) and BV421-labeled anti-Ly6G (IgG2A, #1A8; used at 1 µg/ml; BD Biosciences, Cat. No. 562737). Sections were then incubated for 1 h with an anti-rabbit IgG Alexa Fluor® 532-conjugated detection antibody (used at 1 µg/ml; ThermoFisher Scientific-Molecular Probes, Cat. No. A-11009). After each step, sections were washed with PBS2+, pH 7.4, containing 0.01% (vol/vol) Tween-20® (Merck-Millipore). Correspondent IgG isotype controls (BV421- and Alexa Fluor® 647-labeled rat IgGs from BD Biosciences, respectively, Cat. No. 562602 and 557690, and a purified homemade rabbit IgG) were used. Lungs obtained from Apcs−/− mice were used as control. Sections were mounted with the antifade medium FluorPreserve® Reagent (Merck-Millipore) and analyzed in a sequential scanning mode with a Leica TCS SP8 confocal microscope at Airy Unit 1 (pinhole aperture 95.5 µm) with an oil immersion lens HC PL APO 63X (N.A. 1.4) equipped with Leica Application Suite X software (LASX; version 3.5.5.19976). Images of SAP binding to resting or germinated conidia were obtained after z-stack acquisition using same instrument parameters and image deconvolution by Huygens Professional software (Scientific Volume Imaging B. V.; version 19.10) and presented as medium intensity projection (MIP).
SNP selection and genotyping
Genomic DNA was isolated from whole blood of patients enrolled in the IFIGEN study using the QIAcube automated system (Qiagen). SNPs were selected based on their ability to tag surrounding variants with a pairwise correlation coefficient r2 of at least 0.80 and a minor allele frequency ≥5% using publically available sequencing data from the Pilot 1 of the 1000 Genomes Project for the CEU population. Supplementary Table 3 reports sequences of the primers used in the study. Genotyping was performed using KASPar assays (LGC Genomics) in an Applied Biosystems 7500 Fast Real-Time PCR system (Thermo Fisher Scientific), according to the manufacturer’s instructions. Mean call rate for the SNPs was > 98%. Quality control for the genotyping results was achieved with negative controls and randomly selected samples with known genotypes.
In silico data analysis
Publicly available expression data relative to human and murine myeloid cells were retrieved from the open access platform Gene Expression Omnibus archive (GEO). Data relative to human mononuclear BM cells were retrieved from the GEO data series GSE4251962,63 based on Affymetrix Human Genome U133 Plus 2.0 Array. In this experiment, mononuclear BM cells from heathly donors were enriched for CD34+ and hematopoietic stem and progenitor cells were sorted by FACS. Hematopoietic stem cells (HSCs) and hematopoietic progenitors (HPCs) with the following immunophenotypes were purified as described using a BD FACS Aria ITM cell sorter (BD Bioscience): HSCs, Lin− CD34+ CD38− CD90+ CD45RA−; multipotent progenitors, Lin−, CD34+, CD38− CD90− CD45RA-; common myeloid progenitors, Lin− CD34+ CD38+ CD45RA− CD123+; megakaryocyte-erythroid progenitors, Lin− CD34+ CD38+ CD45RA− CD123−; granulocyte-macrophage progenitors, Lin− CD34+ CD38+ CD45RA+ CD123+. BM populations representing early and late promyelocytes, myelocytes, metamyelocytes, band cells, and BM polymorphonuclear cells were isolated by flow cytometry-based cell sorting: early promyelocytes, Lin− FSChi SSCint CD34− CD15int CD49dhi CD33hi CD11b− CD16−; late promyelocytes, Lin− FSChi SSChi CD34− CD15hi CD49dhi CD33hi CD11b− CD16−; myelocytes, Lin− FSChi SSChi CD34− CD15hi CD49dhi CD33hi CD11bhi CD16−; metamyelocytes, Lin− FSChi SSChi CD34− CD15hi CD49d− CD33− CD11bhi CD16−; band cells, Lin− FSChi SSChi CD34− CD15hi CD49d− CD33− CD11bhi CD16int; polymorphonuclear cells, Lin− FSChi SSChi CD34− CD15hi CD49d− CD33− CD11bhi CD16hi. Data relative to monocytes and macrophages derived from patients affected by acute coronary syndrome were obtained from the GEO data series GSE1143064 based on Affymetrix Human Genome U133 Plus 2.0 Array. In this study, blood samples were obtained from patients with symptoms of acute coronary syndrome who had undergone coronary angiography and who had one stenosis > 50% diagnosed in at least one major coronary artery. RNA samples were extracted from monocyte and monocyte derived macrophages. Data relative to human neutrophils were obtained from the GEO data series GSE303765 based on Affymetrix Human Genome U133A Array. Peripheral blood neutrophils were isolated from septic patients and treated in vitro with LPS or HMGB1. Since no raw data were available, data were processed starting with the gene expression matrix. Expression data of murine macrophage were obtained from the GEO data series GSE293566. Mouse macrophage cultures were obtained from PBMCs isolated from wt mice inoculated with Sendai virus (SeV) or UV-inactivated SeV (UV-SeV). Expression was evaluated with Affymetrix Murine Genome U74A Version 2 Array. Since no raw data were available, data were processed starting with the gene expression matrix. Data of murine neutrophils were obtained from the GEO series GSE2521167 based on Illumina MouseRef-8 v2.0 expression beadchip array. In this study, transcriptional differences of neutrophils isolated from wt and Il-1r1−/− mice after treatment with Ikkβ inhibitors were measured by Illumina MouseRef-8 v2.0 expression beadchip array. All microarray transcriptional data were processed starting with raw files when available, using R statistical environment (version 3.5.2) with the R packages “Biobase” version 2.42.0), “GEOquery” (version 2.50.5). Normalization, probes annotation, gene summarization and differential expression significance assessments were performed with the R package Limma (version 3.38.3). Bulk RNA-Seq data of human peripheral monocytes of COVID-19-infected patients and healthy controls were obtained from the GEO series GSE16035157. Associated transcriptomic data of human peripheral neutrophils derived from the same healthy donor samples were generated using same sequencing technology and protocols57. Briefly, RNA from neutrophils isolated from whole blood by negative selection using MACSxpress® Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec, Germany) was purified with the Direct-zol RNA Microprep or Miniprep Kits (Zymo Research), according to manufacturer’s instructions. Total-RNA-sequencing library preparation was performed starting from 1 ng of total-RNA with the SMART-Seq Stranded Kit (Clontech-Takara). Libraries obtained were qualitatively assessed by using TapeStation 4200 (Agilent) and quantified by Qubit Fluorimeter (Thermofisher). Afterwards, they were multiplexed in equimolar pools and sequenced on a NextSeq-550 Illumina Platform generating at least 80 million 75bp-PE reads per sample. Raw RNA-Seq data of human peripheral neutrophils derived from three healthy donors are deposited and publicly available under the GEO series GSE163533. Raw files were aligned and quantified with STAR (version 2.6.1) on the GRCh38 genome guided by GENCODE annotation (version 33). Gene summarized counts were processed in R, genes whose expression was minor than two reads were removed while the remaining portion was “vs. normalized” with the R package DESeq268 (version 1.22.1). Plots were rendered with the R library “ggplot2” (version 3.3.2).
Statistic
Student’s t-tests were performed after data were confirmed to fulfill the criteria of normal distribution and equal variance. Otherwise Mann–Whitney test was applied. Log-rank (Mantel-Cox) test was performed for comparison of survival curves. Statistical significance of multivariate frequency distribution between groups was also analyzed by Fisher’s Exact test. Analyses were performed with GraphPad Prism version 6 or 7c software. The probability of IPA according to APCS genotypes was determined using the cumulative incidence method and compared using Gray’s test. Cumulative incidences of infection at 24 months were computed with the cmprsk package for R version 2.10.1, with censoring of data at the date of last follow-up visit and relapse and death as competing events. All clinical and genetic variables achieving a P-value ≤ 0.15 in the univariate analysis were entered one by one in a pairwise model together and kept in the final model if they remained significant (P < 0.05). Multivariate analysis was performed using the subdistribution regression model of Fine and Gray with the cmprsk package for R version 2.10.1.
Reporting summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data availability
All data needed to evaluate the conclusions in the paper are present in the paper and/or in the Supplementary Information and Data Source File. Additional data related to this paper may be requested from the authors. Source data are provided with this paper.
Code availability
Bioinformatic methods used for in silico analyses were based on publicly available software, custom scripts might be requested to the authors. Microarray-based computational analyses were performed on publicly available datasets derived from the Gene Expression Omnibus (GEO) platform under the accession ID GSE42519, GSE11430, GSE3037, GSE2935, GSE25211, retrieved following these links: RNA-Seq data were derived from in-house performed sequencing experiments and are publicly available under the accession ID GSE160351 and GSE163533.
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Acknowledgements
The contribution of the European Commission (ERC project PHII-669415; FP7 project 281608 TIMER; ESA/ITN, H2020-MSCA-ITN-2015-676129), Ministero dell’Istruzione, dell’Università e della Ricerca (MIUR) (project FIRB RBAP11H2R9), Associazione Italiana Ricerca sul Cancro (AIRC IG-19014 and IG-21714, AIRC 5 × 1000 −9962 and −21147), the Italian Ministry of Health, the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) (NORTE-01-0145-FEDER-000013 and NORTE-01-0145-FEDER-000023), the Fundação para a Ciência e Tecnologia (FCT) (UIDB/50026/2020, UIDP/50026/2020, PTDC/SAU-SER/29635/2017, PTDC/MED-GEN/28778/2017, CEECIND/04058/2018 and CEECIND/03628/2017), the European Union’s Horizon 2020 research and innovation program under grant agreement no. 847507 and the “la Caixa” Foundation (ID 100010434) and FCT under the agreement LCF/PR/HR17/52190003 is gratefully acknowledged.
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A.D. contributed to the experimental design and conducted experiments, in particular animal model of invasive aspergillosis and in vitro experiments of binding and phagocytosis, and drafted the manuscript. R.P., I.L., Re.P. performed in vivo experiments. C.C. performed the genotyping study and statistical analysis. S.N.M., performed the in silico analyses. F.S.C., E.M. performed flow cytometry. J.F.L., A. Campos, T.M., K.L., J.M., A. Carvalho provided the patient samples and information in the context of the IFIGEN and FUNBIOMICS consortiums. A. Carvalho also participated in the drafting of the manuscript. F.P. prepared fungal culture. T.S. performed confocal microscopy. A.I., J.D.L., B.B. contributed to the scientific discussion of the project. C.G., M.B. contributed to the scientific discussion and participated in the drafting of the manuscript. A.C. conceived study of Apcs genotyping in patients and identified the relationship between genetic variation of Apcs and susceptibility to IPA and participated in drafting the manuscript. A.M. played a key role in designing and supervision of the study and drafted the final version of the paper.
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A.M. and A.D. are inventors of a patent on SAP (WO2020127471). The other authors do not report competing interests.
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Doni, A., Parente, R., Laface, I. et al. Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus. Nat Commun 12, 3739 (2021). https://doi.org/10.1038/s41467-021-24021-y
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DOI: https://doi.org/10.1038/s41467-021-24021-y
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