RORγt+ Treg to Th17 ratios correlate with susceptibility to Giardia infection

Yordanova, Ivet A.; Cortés, Alba; Klotz, Christian; Kühl, Anja A.; Heimesaat, Markus M.; Cantacessi, Cinzia; Hartmann, Susanne; Rausch, Sebastian

doi:10.1038/s41598-019-56416-9

Download PDF

Article
Open access
Published: 30 December 2019

RORγt⁺ Treg to Th17 ratios correlate with susceptibility to Giardia infection

Ivet A. Yordanova ORCID: orcid.org/0000-0003-4119-7308¹,
Alba Cortés²,
Christian Klotz³,
Anja A. Kühl⁴,
Markus M. Heimesaat⁵,
Cinzia Cantacessi ORCID: orcid.org/0000-0001-6863-2950²,
Susanne Hartmann¹ &
…
Sebastian Rausch¹

Scientific Reports volume 9, Article number: 20328 (2019) Cite this article

2585 Accesses
13 Citations
2 Altmetric
Metrics details

Subjects

Abstract

Infections with Giardia are among the most common causes of food and water-borne diarrheal disease worldwide. Here, we investigated Th17, Treg and IgA responses, and alterations in gut microbiota in two mouse lines with varying susceptibility to Giardia muris infection. Infected BALB/c mice shed significantly more cysts compared with C57BL/6 mice. Impaired control of infection in BALB/c mice was associated with lower Th17 activity and lower IgA levels compared with C57BL/6 mice. The limited metabolic activity, proliferation and cytokine production of Th17 cells in BALB/c mice was associated with higher proportions of intestinal Foxp3⁺RORγt⁺ regulatory T cells and BALB/c mice developed increased RORγt⁺ Treg:Th17 ratios in response to G. muris infection. Furthermore, G. muris colonization led to a significantly reduced evenness in the gut microbial communities of BALB/c mice. Our data indicate that differential susceptibility to Giardia infections may be related to RORγt⁺ Treg controlling Th17 activity and that changes in the microbiota composition upon Giardia infection partially depend on the host background.

Immune microniches shape intestinal Treg function

Article Open access 03 April 2024

Yisu Gu, Raquel Bartolomé-Casado, … Fiona Powrie

A host–microbiota interactome reveals extensive transkingdom connectivity

Article 20 March 2024

Nicole D. Sonnert, Connor E. Rosen, … Noah W. Palm

Microbiota in health and diseases

Article Open access 23 April 2022

Kaijian Hou, Zhuo-Xun Wu, … Zhe-Sheng Chen

Introduction

Infections with the intestinal protozoan parasite Giardia lamblia remain a highly prevalent cause of food- and water-borne diarrheal disease across the world. Recent data indicate that over 183 million cases of giardiasis occur annually across the globe, with a disease burden of almost 172,000 Disability Adjusted Life Years (DALYs)¹. G. lamblia accounts for ~35–37% of water-borne disease outbreaks and is estimated to cause 0.5–5.4% of cases of diarrhea in children under 5 years of age in both high- and low-income countries^2,3. Giardia trophozoites are typically non-invasive and preferentially colonize the upper small intestinal tract, where they attach in dense foci to the epithelial cell layer, a mechanism thought to contribute to localized and generally mild immunopathology^4,5. Giardiasis commonly causes few signs of intestinal inflammation⁶, however some patients do develop diverse clinical manifestations, such as chronic diarrhea, abdominal pain, fatigue and malabsorption⁶. Studies surveying Giardia infection intensity in human patients indicate a wide variation in fecal cyst shedding rates in children⁷. Similarly, studies in dairy calves infected with G. lamblia indicate significant individual variations in fecal cyst shedding⁸. The potential factors influencing such differences in parasite loads, overt immunopathology or the development of clinical symptoms are yet to be characterised.

Ex vivo stimulation of blood cells, small intestinal lamina propria (siLP) lymphocytes or intestinal epithelial lymphocytes from G. lamblia-infected patients leads to the production of a number of pro-inflammatory cytokines, including IL-17A^9,10. Recent work in experimental mouse models has demonstrated that Th17 cells and IL-17A production are crucial players in the efficient control of Giardia infection^11,12,13. IL-17A typically drives neutrophil recruitment, antimicrobial peptide secretion and supports the expression of the polymeric Ig receptor (pIgR) by intestinal epithelial cells, which is necessary for IgA transport into the intestinal lumen^11,13,14. IgA secretion represents another key immune protective mechanism against Giardia, as mice deficient in B cells, IgA or pIgR fail to control infection with G. lamblia or G. muris, the species adapted to the murine host^11,15,16.

The high prevalence of asymptomatic Giardia infections and the minimal immunopathology commonly observed during experimental giardiasis suggests that immune-regulatory mechanisms may participate in controlling the pro-inflammatory potential of Th17 cells, possibly at the expense of efficient elimination of infection⁸. Regulatory T cells (Treg) expressing the transcription factor Foxp3 are pivotal for the maintenance of homeostasis and regulation of overt inflammatory processes¹⁷. In recent years it was shown that Foxp3⁺ Treg adopt functional specialization by expressing the transcription factors T-bet, GATA-3 or RORγt associated with the Th1, Th2 and Th17 effector lineages, respectively. These effector-like Treg populations were shown to efficiently co-localize with the respective effector T cell subsets and to constrain inflammatory responses^18,19,20. Hence, distinct subsets of Treg are a key factor balancing immune responses at mucosal surfaces²¹. RORγt⁺ Treg have been characterized as peripherally-induced and highly enriched in the small intestinal and colonic lamina propria^20,22. Their differentiation is primarily dependent on commensal microbiota signaling and their absence leads to elevated IL-17A expression by intestinal T cells^20,23,24.

Intestinal Treg heterogeneity and associations between Treg phenotypes and Th17 cell activity have not been addressed in the context of Giardia infections to date. Here, we assessed Th17, Treg and IgA responses and the microbiota composition in two mouse lines with differential susceptibility to G. muris infection. We found that BALB/c mice releasing moderately higher cyst numbers display poor Th17 activity, increased Foxp3⁺RORγt⁺ Treg to Th17 cell ratios, limited IgA production and more pronounced changes in the microbiota structure compared to C57BL/6 mice, which restrict Giardia replication more readily.

Results

Differential control of Giardia infection is associated with Th17 activity

Over the course of 6 weeks, BALB/c mice displayed higher fluctuations in cyst shedding and shed significantly more cysts in the second week after infection compared with C57BL/6 mice. (Fig. 1a). The moderately higher cyst numbers released by infected BALB/c mice were not associated with changes in body weight development (Fig. 1b). Furthermore, BALB/c mice displayed no signs of intestinal inflammation. In contrast, enteritis scores were slightly elevated in C57BL/6 mice irrespective of the infection status, which was due to mild leukocyte infiltrations and/or slight blunting of villi (Fig. 1c). Previous studies demonstrated that the efficient control of Giardia infection depends on Th17 cells secreting IL-17A^11,12. Asking if the moderate difference in susceptibility to Giardia infection correlated with the activity of Th17 cells, we surveyed RORγt and IL-17A expression by CD4⁺ T cells in BALB/c and C57BL/6 mice at steady state and two weeks post G. muris infection. C57BL/6 mice harbored higher frequencies of Th17 cells expressing IL-17A and RORγt within CD4⁺ lymphocytes isolated from the small intestinal lamina propria (siLP) and Peyer’s patches (PP) compared to BALB/c mice (Fig. 1d,e). Of note, the frequencies of small intestinal CD4⁺ effector cells expressing RORγt⁺ and IL-17A⁺ remained stable upon infection in both mouse lines (Fig. 1d,e) despite significantly higher proportions of siLP Th17 cells expressing the proliferation marker Ki-67 in infected C57BL/6 mice (Fig. 1f). We hence assessed the expression of the tissue retention markers CD69 and CD103 by small intestinal Th17 cells to see if this discrepancy might be due to recirculation of Th17 cells from gut tissue in infected C57BL/6 mice. However, small intestinal Th17 cells of both mouse lines similarly expressed CD69 and CD103 at steady state and upon infection (Suppl. Figure 1a,b).

Next, we investigated if the stronger IL-17A expression detected in small intestinal CD4⁺ of C57BL/6 mice was reflected in vivo by quantifying IL-17A gene transcription in the small intestine. IL-17A transcript levels were significantly higher in the ileum of naive C57BL/6 compared with naïve BALB/c mice. However, corroborating the unaltered IL-17A protein expression detected in intestinal CD4⁺ T cells upon restimulation, IL-17A transcription in duodenum, jejunum and ileum was not augmented two weeks after G. muris infection (Fig. 1g).

To test if differences in parasite-specific Th17 responses were associated with the differential control of Giardia replication in the two mouse lines, we stimulated cells isolated from spleen, mLN and PP of naïve and G. muris-infected BALB/c and C57BL/6 mice with trophozoite antigen derived from the two G. lamblia strains GS (assemblage B) and WB6 (assemblage A) or with anti-CD3/CD28 antibodies as a positive control. IL-17A responses triggered by anti-CD3/CD28 antibodies did not differ depending on the infection status in cultures of spleen, mLN or PP cells (Fig. 1h and data not shown). Parasite-specific IL-17A responses to both types of antigen were detected in spleen cell cultures of several infected C57BL/6 mice, while the corresponding cultures of most infected BALB/c mice responded poorly to the antigens (Fig. 1h). Interestingly, IL-17A production in response to the parasite antigens was only detected with spleen cells, whereas parallel cultures of mLN and PP cells did not react to parasite antigens (data not shown).

Taken together, these data indicate that lower cyst shedding by G. muris infected C57BL/6 mice relates to constitutively higher frequencies of intestinal Th17 cells and the more rapid development of parasite-specific IL-17A responses to Giardia infection compared to BALB/c mice

Restricted Th17 responses correlate with elevated frequencies of intestinal RORγt⁺ Treg

The poor Th17 activity in BALB/c compared with C57BL/6 mice at steady state and during G. muris infection prompted the question if this was associated with differences in the regulatory T cell (Treg) compartment. Frequencies of Foxp3⁺ Treg within the CD4⁺ T cell population were similar in the small intestine of both mouse lines and did not change during infection in BALB/c and C57BL/6 mice (Fig. 2a). Confirming the flow cytometry data, similar numbers of CD4⁺ T cells and CD4⁺Foxp3⁺ Treg were detected for all groups in cross sections of duodenum, jejunum and ileum (Fig. S1c,d). Peripherally induced Foxp3⁺Helios⁻ Treg cells co-expressing RORγt have previously been reported to display superior suppressive activity compared to conventional RORγt⁻ Treg^22,25. We found that Foxp3⁺ Treg isolated from the small intestine of BALB/c mice comprised significantly more RORγt⁺ cells compared with Treg derived from C57BL/6 mice (Fig. 2b,c). In line with previous reports^22,25, RORγt⁺ Treg largely lacked Helios expression, indicating their extra-thymic origin (Fig. S1e). Next, we surveyed the uptake of fluorescently-labelled palmitate (Bodipy FL16) as a measure of cellular metabolic activity. In both infected mouse lines, RORγt⁻ and RORγt⁺Foxp3⁺ Treg acquired higher levels of palmitate compared to Foxp3⁻RORγt⁻CD4⁺ cells (Fig. S1f). CD4⁺ T cells isolated from the small intestine of G. muris infected C57BL/6 mice, but not BALB/c mice, comprised a brightly FL16-labeled population, which predominantly originated from RORγt⁺ Th17 cells expressing the early T cell activation marker CD69 (Fig. 2d). G. muris infection led to a significant increase in palmitate uptake by Th17 cells in infected C57BL/6 mice compared with naive controls and with infected BALB/c mice (Fig. 2e). Furthermore, BALB/c mice displayed significantly higher ratios of RORγt⁺ Treg to Th17 cells at steady state compared with C57BL/6 mice and this ratio increased further in response to G. muris infection in BALB/c, but not C57BL/6 mice (Fig. 2f). Consequently, we detected a significant negative correlation between the ratio of RORγt⁺ Treg to Th17 cells and the metabolic activity, as well as the proliferative response of intestinal Th17 cells (Fig. 2g,h). In conclusion, G. muris infection induces a significant shift in favor of RORγt⁺ Treg in BALB/c, but not C57BL/6 mice. In addition, high ratios of RORγt⁺ Treg to Th17 cells correlate with poor activity in local Th17 cells in Giardia infected mice.

Poor ILC3 activity in BALB/c mice at steady state and during G. muris infection

A previous study reported the increased expression of IL-17A by innate immune cells along with CD4⁺ T cells during G. muris infection in C57BL/6 mice¹¹. We hence asked if BALB/c and C57BL/6 mice differed in IL-17A responses by non-CD4⁺ T cells. Contrasting the higher frequencies of Th17 cell in the small intestines of C57BL/6 mice, we detected significantly more CD90⁺CD4⁻RORγt⁺ group 3 innate lymphoid cells (ILC3) in naïve and infected BALB/c mice (Fig. 3a,b). However, as seen for Th17 cells, small intestinal ILC3 of naïve and infected BALB/c mice poorly produced IL-17A following ex vivo stimulation compared with those from C57BL/6 mice (Fig. 3a,c). ILC3 from PP mirrored this pattern of IL-17A responses (Fig. S2a), whereas IL-17A production by mLN-derived ILC3 was similar in both mouse lines (Fig. S2b,c). Thus, higher G. muris cyst excretion by BALB/c mice coincides with poor IL-17A production by Th17 cells and ILC3 in the local vicinity of the parasite.

Systemic and local IgA levels mirror small intestinal Th17 activity at steady state and during Giardia infection

Intestinal IgA is a key host protective factor in giardiasis^11,15,16. We hence investigated if differential parasite control by C57BL/6 compared with BALB/c mice coincided with differences in their local and systemic antibody responses. We therefore assessed the frequencies of B220⁻IgA⁺ plasma cells (PC) in siLP and B220⁺CD19⁺IgA⁺ B cells in PP, as well as serum and intestinal IgA titers. Indeed, siLP isolates of C57BL/6 mice comprised higher frequencies of B220⁻IgA⁺ plasma cells compared with BALB/c cell isolates, reaching significance two weeks post-G. muris infection (Fig. 4a). In contrast, similar frequencies of IgA⁺ B cells were present in PP of both mouse lines at steady state and following infection (Fig. 4b). Serum IgA levels were similar at steady state and two weeks post infection, however, both naive and infected C57BL/6 mice had significanty more IgA in serum compared to BALB/c mice (Fig. 4c). Higher serum IgA levels were accompagnied by significantly more IgG2b in serum of naïve C57BL/6 compared to BALB/c mice and IgG2b levels increased further in response to G. muris infection in the C57BL/6, but not the BALB/c line (Fig. 4d). To estimate possible differences in secretory IgA transport into the intestinal lumen, we quantified IgA released by intestinal tissue explants and assessed pIgR transcript levels in small intestinal tissue. Duodenal explants isolated from naïve CD57BL/6 mice released significantly higher amounts of IgA compared to tissue from BALB/c mice and a similar trend was maintained during infection (Fig. 4e). IgA levels secreted by jejunum and ileum explants were similar for all groups (Fig. 4e). In trend, pIgR transcript levels were increased in duodenum, jejunum and ileum of C57BL/6 compared with BALB/c mice (Fig. 4f). As both IgA and IgG2b class switching are supported by the cytokine TGF-β^26,27, we surveyed TGF-β mRNA and protein levels in intestinal samples of BALB/c and C57BL/6 mice and found no evidence for differential TGF-β transcription or TGF-β protein levels depending on the host background and infection status (Fig. 4g and data not shown). Thus, more efficient parasite control by C57BL/6 mice coincided with a more prominent population of intestinal IgA⁺ PC and systemically elevated IgA and IgG2b levels at steady state and during G. muris infection.

Alterations in microbiota structure resulting from G. muris infection differ depending on the host genetic background

The gut microbiota is pivotal for the development of intestinal Th17 and IgA responses which, in turn, regulate the composition of the microbial community^28,29. We hence asked if the differential Th17 and IgA responses detected in BALB/c compared with C57BL/6 mice under homeostasis and upon G. muris infection were associated with distinct microbiota signatures. Comparing the fecal microbiome of naïve BALB/c and C57BL/6 mice, we detected differences in the microbiota structure between the two mouse lines (Fig. S3a,b). In particular, microbial beta diversity (i.e., differences in microbial species composition between groups) measured by ANOSIM on Bray-Curtis dissimilarities, was significantly lower in C57BL/6 than in BALB/c mice (p = 0.007; Fig. S3c). Furthermore, whilst no significant differences were detected in overall alpha diversity (measured through the Shannon index) and microbial richness (i.e., the number of species composing a given microbial community), a significantly lower bacterial evenness (i.e., the relative abundance of each microbial species within a given microbial community; p = 0.035, ANOVA) was observed in C57BL/6 compared with BALB/c mice (Fig. S3d). Comparison of individual microbial taxa abundances by Linear discriminant analysis Effect Size (LEfSe) and negative binomial distribution (DESeq. 2) revealed significant differences (from phylum to genus level) between the two mouse lines, including several families/genera within the order Clostridiales (Table S1, S2).

Analysis of the microbiome composition of G. muris-infected BALB/c and C57BL/6 mice in comparison with their respective uninfected controls revealed significant alterations of the gut microbial community of infected BALB/c, but not C57BL/6 mice, as detected by unsupervised Principal Coordinates Analysis (PCoA, on Bray-Curtis dissimilarities) (Fig. 5a). The observed alterations in gut microbial community profiles of infected BALB/c mice were associated with a decrease in overall alpha diversity (measured through Shannon index; p = 0.038, ANOVA), which was driven by a significant reduction in microbial evenness (p = 0.0039, ANOVA), whilst species richness remained unaltered (Fig. 5b). In contrast, no alterations in alpha diversity were observed in the microbiota of C57BL/6 mice upon infection (Fig. 5b). Microbial beta diversity measured by ANOSIM on Bray-Curtis dissimilarities did not reveal significant differences between infected and uninfected mice of either mouse lines (Fig. S4).

Importantly, populations of gut bacteria significantly impacted by G. muris colonization according to DESeq. 2 and LEfSe varied between the two mouse lines (Fig. 6a, Tables S3, S4). G. muris infection led to an increase of Coriobacteriales (fold change = 4.37, p = 0.013) and a minor expansion of Erysipelotrichaceae (fold change = 3.21, p = 0.034) in BALB/c, but not in C57BL/6 mice (Fig. 6b). Surprisingly, segmented filamentous bacteria (SFB, genus Candidatus Arthromitus) were identified as the most prominently expanding taxon in infected BALB/c mice (Fold change = 10.38, p = 0.001) (Fig. 6b), whereas infected C57BL/6 mice displayed a significant increase in the abundance of the Akkermansiaceae family (fold change = 8.72, p = 0.043) (Fig. 6b). The abundance of Candidatus Saccharimonas was significantly reduced in both mouse lines upon G. muris infection (fold change = −4.45/−3.69, p = 0.007/0.022) (Fig. 6b). Furthermore, significant alterations in the composition of the order of Clostridiales were detected in infected BALB/c and C57BL/6 mice. Specifically, we detected a reduction in populations of the genera Intestinimonas (fold change = −5.31, p = 0.00004), Lachnospiraceae UCG006 (fold change = −3.43, p = 0.01), Ruminiclostridium 5 (fold change −2.92, p = 0.01) and Blautia (fold change = −2.68, p = 0.02) in infected BALB/c mice, whereas Blautia expanded in infected C57BL/6 mice (fold change = 4.21, p = 0,002) (Fig. 6b). Finally, infected C57BL/6 mice displayed a highly significant decrease in the abundance of Gastranaerophilales (Fold change = −17.96, p = 0.00001; DESeq. 2) (Fig. 6b). LEfSe analysis (Table S4) supported the results obtained by DESeq. 2 (except for Blautia) and revealed that, within the order Coriobacteriales, the family Eggerthellaceae, and the genus Enterorhabdus in particular, were significantly more abundant in the gut of infected BALB/c mice, whilst the genus Akkermansia was associated with G. muris infection in C57BL/6 mice (Table S4).

Further 16S rRNA-based qPCR analysis of the fecal microbiota composition showed that the total bacterial load remained stable in both mouse lines upon G. muris infections (Fig. 7a) and confirmed that the abundance of members of Proteobacteria (Enterobacteria, Fig. 7b), Actinobacteria (Bifidobacteria, Fig. 7c), Bacteroidetes (Bacteroides/Prevotella spp. and Mouse Intestinal Bacteroidetes, MIB; Fig. 7d,e) and Firmicutes (Enterococci, Lactobacilli, Clostridium coccoides/leptum groups, Fig. 7f–i) remained unaltered during G. muris infection and were comparable in abundance between naive and infected BALB/c and C57BL/6 mice.

Discussion

Our study shows that mice with a different genetic background display differential susceptibility towards G. muris colonization. In accordance with previous reports of prolonged and elevated G. muris cysts excretion and higher numbers of G. lamblia trophozoites in infected BALB/c compared with C57BL/6 mice^30,31, we detected a moderately higher cyst release in the BALB/c line compared with C57BL/6 mice.

The work of several groups has demonstrated that Th17 responses are pivotal for the control of murine Giardia infections^11,12,13. Furthermore, a retrospective survey of disease course and memory responses of human patients after clearance of G. lamblia infection revealed that individuals that had more readily controlled giardiasis comprised more IL-17A-producing cells in peripheral blood¹⁰. In line with these reports, we show here that C57BL/6 mice harboring relatively high frequencies of small intestinal Th17 cells together with ILC3 contributing to IL-17A production at steady state and upon infection, controlled G. muris infection more efficiently compared to BALB/c mice. Increased cyst shedding in BALB/c mice was not associated with signs of morbidity such as weight loss or small intestinal inflammation, confirming previous reports on minimal inflammation induced by Giardia infection^32,33. Of note, stronger Th17 activity detected in C57BL/6 mice correlated with mild signs of enteritis in these mice, albeit this was not altered upon G. muris infection.

Comparing the two mouse lines at steady state and two weeks (or 7 days, data not shown) post infection, we detected no increase in intestinal IL-17A transcript levels and found stable frequencies of IL-17A producing Th17 cells and ILC3 in the small intestine and PP. However, previous studies clearly demonstrated elevated IL-17A mRNA levels in small intestinal tissue as early as 7 days post G. muris infection in C57BL/6 mice, coinciding with more prominent IL-17A expression by small intestinal CD4⁺ T cells and innate immune cells^11,12. Whether this discrepancy relates to the different origin of experimental animals and their microbiota composition is not clear. Of note, the stable frequencies of IL-17A⁺ Th17 detected in the small intetsines of naïve and infected C57BL/6 mice reported here exceed the levels found in the study by Dann & colleagues¹¹, which may have masked a more subtle rise in the activity of intestinal Th17 cells in infected mice in our study.

Contrasting the unaltered local IL-17A expression, we detected elevated proliferation and fatty acid uptake to occur selectively in small intestinal Th17 cells from infected C57BL/6 mice, indicative of more active Th17 cells in these mice. Whether these comprise early stage RORγt⁺ effector cells recently attracted to the infected gut, yet producing low levels of IL-17A due to the paucity of signals educating them for effector cytokine expression³⁴, remains to be established. Alternatively, Th17 cells present in higher frequencies in naïve C67BL/6 compared to BALB/c mice may receive signals sufficient for local expansion, but insufficient for the launch of the full effector program. According to our data, BALB/c mice appear even more reluctant in that respect, since these lack any sign of elevated Th17 activity within two weeks of Giardia infection. Furthermore, the basis for stable frequencies of small intestinal Th17 cells detected along with increased Th17 cell proliferation in infected C57BL/6 mice reported here deserves further investigation. The similar expression of CD103 and CD69 important for T cell retention in peripheral tissues^35,36 by small intestinal Th17 cells from naïve and infected mice paired with similar frequencies of systemic Th17 cells in spleen (data not shown) does not support the idea that small intestinal Th17 cells of infected C57BL/6 mice might more readily recirculate from gut tissue. On the other hand, we detected Giardia-specific IL-17A production by spleen cells, but not mLN and PP cells of infected C57BL/6 mice controlling parasite replication more efficiently. This finding might reflect the release of highly functional parasite-specific cells from the gut or associated lymphoid tissues into systemic circulation. Whether the apparent lack of parasite-specific responses in PP and mLN of both mouse lines results from the effective suppression of cytokine production by local regulatory populations, such as Treg, remains to be addressed.

We found that elevated RORγt⁺ Treg to Th17 ratios were associated with poor IL-17A production by Th17 cells and ILC3 and correlated with the poor proliferation and metabolic activity of intestinal Th17 cells in infected BALB/c mice. Foxp3⁺RORγt⁺ cells represent a stable Treg subset enriched in the intestinal lamina propria and potently suppress Th17, Th1 and Th2-associated inflammatory programs^20,22,25,37. It is hence conceivable that the elevated proportions of RORγt⁺ Treg contributed to impaired parasite control in BALB/c mice. In support of this interpretation, a previous study demonstrated that mice selectively lacking the Foxp3⁺RORγt⁺ Treg subset develop elevated intestinal IL-17A responses at steady state and experience exacerbated immunopathology in a chemically-induced colitis model²⁰. The relative paucity of RORγt⁺ Treg detected in C57BL/6 mice might hence be linked to the mild signs of inflammation detected in this mouse strain irrespective of Giardia infection. Along those lines, RORγt⁺ Treg expressing high levels of IL-10^22,24 might be important players restricting microbial dysbiosis and gut inflammation recently reported to develop in Giardia infected mice in the absence of IL-10³⁸.

The generation of RORγt⁺ Treg depends on the presence of gut microbes, with clostridial species, but also other members of Firmicutes, Bacteroidetes and Proteobacteria supporting their differentiation^20,22,25. Recent work in C57BL/6 mice has demonstrated that infection with G. lamblia leads to shifts in the composition of the commensal microbiota along the entire gastrointestinal tract, despite the predominant localization of trophozoites to the proximal small intestine^4,39. Barash and colleagues detected an enrichment of Proteobacteria paralleled by the depletion of members of the Firmicutes (Clostridiales) in the small, as well as large intestine within two weeks of G. lamblia infection³⁹. We hence surveyed the abundance of microbial taxa assigned with the ability to expand Treg, such as Lactobacilli, Bifidobacteria and Clostridia^40,41,42 via 16S rRNA-based qPCR and found no differences depending on the mouse strain or infection status. However, high-throughput Illumina sequencing revealed that the overall microbiota structure of BALB/c, but not C57BL/6 mice, was significantly altered upon G. muris infection and that the clostridia Intestinimonas, Ruminiclostridium 5 and Blautia moderately decreased in infected BALB/c mice. Infected C57BL/6 mice also displayed moderate changes in the abundances of Clostridia members, namely an increase of Clostridiaceae 1 and Blautia. Whether the subtle changes between naïve and infected mice concerning the composition of Clostridiales relate to the distinct RORγt⁺ Treg to Th17 cell ratios detected in our study and provide the basis for the distinct phenotypical profiles of small intestinal Treg awaits further investigation.

Gut microbes have been extensively shown to modulate inflammatory and regulatory mucosal immune responses^28,43 and are likely to affect innate and adaptive anti-Giardia immune responses. The generation and accumulation of Th17 cells found in the intestine at steady state depends to a large extent on the microbial colonization of the gut and is potently induced by SFB primarily colonizing the ileum^44,45. Here, we observed similar abundances of SFB in naïve BALB/c and C57BL/6 mice, however an earlier study provided evidence for the impact of the microbiome composition and, possibly, SFB colonization on Giardia control. Singer & Nash showed that C57BL/6 mice provided by two vendors (later shown to differ in SFB colonization⁴⁴), differed in susceptibility to Giardia infection⁴⁶. More recently, Paerewijck and colleagues demonstrated that neonate mice infected with G. muris displayed delayed IL-17A responses and parasite control⁴⁷. As SFB colonization and the accumulation of intestinal Th17 cells typically occurs in mice at the time of weaning⁴⁸, the delayed control of G. muris seen after neonatal infection suggests that Th17 responses triggered by these and other microbes later in life provide an environment equipped for the partial control of Giardia infections. Of note, comparably poor Th17 responses of BALB/c mice to gut microbes were shown to result from minor IL-1β production by small intestinal dendritic cells, translating to poor production of serum amyloid A (SAA) by epithelial cells and the more restrained activity of Th17 cells compared to C57BL/6 mice⁴⁵. Hence, the activation of microbe-specific Th17 cells may precede the activity of Giardia-specific Th17 cells and this circuit might restrict Giardia replication more efficiently in infected C57BL/6 mice already equipped with a more prominent intestinal Th17 cell population. Whether the increased susceptibility of BALb/c mice to Giardia infection is is related to their poor IL-1β expression in response to microbes attaching to the gut epithelium⁴⁵, to the higher proportions of RORγt⁺ Treg efficiently restricting the activation of T cells or to peculiarities in their gut microbiota will be assessed in future studies.

Our finding of a significant expansion of SFB in G. muris-infected BALB/c, but not C57BL/6 mice displaying higher frequencies of IL-17A producing Th17 cells might point to the preferential activity of regulatory circuits in Giardia infected mice. As the degree of SFB colonization is regulated by IL-17A and IgA^49,50 it will be interesting to assess if Giardia infection further stunts Th17 and IgA responses directed against gut microbes in BALB/c mice, permitting the expansion of species colonizing the epithelial layer. Of note, two recent reports demonstrated that Giardia infection promotes the secretion of antimicrobial factors in C57BL/6 mice coinfected with C. rodentium or E. coli, which attenuates pathology induced by bacterial attachment to the epithelial layer^51,52. Whether such effects are less pronounced on the BALB/c background is not assessed yet.

Finally, in addition to the distinct Th17 profile, naïve and infected C57BL/6 mice displayed significantly more intestinal IgA⁺ plasma cells, higher serum IgA and IgG2b levels, a trend for elevated pIgR expression along the small intestine as well as slightly higher IgA secretion by duodenal tissue explants compared with BALB/c mice. Elevated IgG2b next to IgA levels have been reported previously for G. muris infected C57BL/6 mice⁵³. The increase in serum IgG2b levels in infected C57BL/6 mice may reflect the more rapid immune activation detected as based on Giardia-specifc IL-17A production. Our attempts to detect Giardia-specific antibody responses were not successful. However, the lack of parasite-specific IgA and IgG2b in sera after two weeks of Giardia infection is in line with a previous study detecting parasite-specific IgA responses no earlier than three weeks post-infection⁵⁴. Furthermore, stable total IgA levels in intestinal secretions reported within four weeks post-infection for G. muris-infected BALB/c corroborate our finding of relatively stable IgA levels in both serum and intestinal secretions of BALB/c and C57BL/6 mice at two weeks post-infection. Taken together, the data presented here suggest that the differential susceptibility detected for C57BL/6 and BALB/c mice early after G. muris infection are most likely independent of differences in parasite-specific antibody production. Naturally, this does not exclude a differential humoral response developing on different genetic backgrounds may affect the control of Giardia at later stages of the infection.

In conclusion, despite discrepancies concerning the local responses of Th17 and innate cells seen in our study compared with previous reports, our finding of distinct patterns of general and parasite-specific Th17 activity linked to the differential susceptibility of two host types supports the established concept of Th17 activity being important for the timely control of G. muris infection. Furthermore, our study reveals that G. muris infection is associated with more prominent shifts in the microbiota composition in BALB/c mice experiencing higher parasite loads, which includes the thriving of SFB sharing the epithelial niche with Giardia trophozoites. The mechanisms deployed by RORγt⁺ Tregs possibly influencing Th17 cell activity during giardiasis and the interplay of microbial signatures and anti-Giardia immune responses would therefore merit more in-depth investigations. Furthermore, it will be interesting to see if deficits in microbes supporting homeostatic Th17 responses might be linked to poor control of giardiasis seen in human patients and to investigate which factors support the thriving of potentially pathogenic microbes probably fuelling the symptoms of intestinal irritation observed in symptomatic giardiasis patients.

Materials and Methods

Mice and G. muris infections

Female BALB/c and C57BL/6 wild-type mice were purchased from Janvier Labs (Saint-Berthevin, France) and maintained under standard specific pathogen-free conditions. All animal experiments were performed in accordance with the National Animal Protection Guidelines and approved by the German Animal Ethics Committee for the Protection of Animals (G0113/15, H0438/17). G. muris cysts were originally purchased from Waterborne, Inc. (USA) and were later maintained by passage in BALB/c mice. Cysts were isolated from fresh fecal samples via a sucrose gradient, as described previously⁵⁵ and were administered to mice via oral gavage at a dosage of 1,000 cysts per mouse suspended in 200 μl distilled water. After two weeks, G. muris-infected mice and age/sex-matched naïve controls were sacrificed by isoflurane inhalation, followed by cervical dislocation. For faecal cyst counts, 3–4 faecal pellets were collected individually from each mouse 3 times a week, followed by weighing and homogenization in 2 mL distilled water. Cyst numbers were counted in a Neubauer hemocytometer chamber and for weekly cyst shedding over the course of a 6 week infection period as presented in Fig. 1a, the mean value of cyst shedding was calculated across the three collection time points per week.

Preparation of single cell suspensions

Spleens, Peyer’s patches and mesenteric lymph nodes were isolated and kept in cold RPMI 1640 medium, containing 1% FCS, 100 U/ml penicillin and 100 μg/ml streptomycin (all from PAA, Pasching, Austria). To prepare single cell suspensions, PP were pre-digested in medium with 0.1 mg/ml liberase TL (Roche, Basel, Switzerland) and 0.1 mg/ml DNase (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C on a shaker for 30 min. Spleen, PP and mLN tissues were then forced through 70 μm cell strainers (BD Biosciences, Heidelberg, Germany). Cell numbers were determined using an automated CASY cell counter (Roche-Innovatis, Reutlingen, Germany). Small intestinal lamina propria cells were isolated as previously described⁵⁶.

Flow cytometry

The following antibodies were used for the detection of surface and intracellular markers: CD4 (clone RM4–5), CD69, (clone H1.2F3), CD90.2 (clone 30H12), CD103 (clone 2E7), Foxp3 (clone FJK-16s), RORγt (clone Q31-378), Ki-67 (clone SolA15), Helios (clone 22F6), IL-17A (clone TC11-18H10), CD45R/B220 (clones RA3-6B2 and RA3-B2), CD19 (clone ID3), IgA (clone mA-6E1), IgM (clone eB121-15F9), CD11b (clone M1/70), CD11c (clone HL3), CD3e (clone 145-2C11), Nkp46 (clone 29A1.4), CCR6 (clone 29-2L17). Non-specific binding was prevented by addition of CD16/CD32 blocking reagent (clone 2.4 G). All antibodies were purchased from eBioscience and Biolegend. Dead cells were excluded using eFluor780 fixable viability dye (Thermo Fisher, Waltham, USA). For intracellular staining of cytokines and transcription factors, cells were fixed and permeabilized using the Fixation/Permeabilization kit and Permeabilization buffer from ThermoFisher/eBioscience. Bodipy FLC16 (ThermoFisher/Invitrogen) labeling was performed at 1 μM in PBS containing 1% FCS for 20 min at 37 °C. Samples were analyzed on a Canto II flow cytometer (BD Biosciences, Heidelberg, Germany) and the data was analyzed using FlowJo software Version 10 (Tree star Inc., Ashland, OR, USA).

Quantitative Real-Time PCR (qRT-PCR)

At necropsy, 0.5 cm tissue snips were excised from duodenal, jejunal and ileal sections of the small intestinal tract. RNA was isolated using the innuPREP RNA kit according to the manufacturer’s instructions (Analytic Jena, Germany). 2 μg of RNA was then reverse-transcribed to cDNA using a High Capacity RNA-to-cDNA kit (Applied Biosystems, Foster City, CA, USA). Relative gene expression was determined via quantitative real-time PCR (qRT-PCR) using 10 ng of cDNA and FastStart Universal SYBR Green Master Mix (Roche). Primer pairs used for gene amplification are as follows: GUSB (F 5′-GCTCGGGCAAATTCCTTTC-3′; R 5′-CTGAGGTAGCACAATGCCCA-3′), IL-17A (F 5′-ACTACCTCAACCGTTCCACG-3′; R 5′-TTCCCTCCGCATTGACACAG-3′), pIgR (F 5′-ACCAATGGTGACTCTCGCTG-3′; R 5′-AGGTTTGGCTCCCTTGTAGC-3′) and TGF-β (F 5′-CTGCTGACCCCCACTGATAC-3′; R 5′-AGGAGACGGAATACAGGGCT-3′). Efficiencies for each primer pair were determined by generating a standard curve, mRNA expression was normalized to the housekeeping gene β-glucuronidase (GUSB) and was calculated by the Roche Light Cycler 480 software.

In vitro stimulation of splenocytes with Giardia lamblia trophozoite antigen

For assessment of parasite-specific IL-17A responses, 5 × 10⁵ splenocytes from naïve and G. muris-infected BALB/c and C57BL/6 mice were plated on a round-bottom 96-well cell culture plate in 300 uL RPMI 1640 medium, containing 10% FCS, 100 U/ml penicillin and 100 μg/ml streptomycin (all from PAA, Pasching, Austria). The splenocytes were stimulated with either anti-CD3/CD28 antibodies (1ug/mL), Giardia lamblia GS strain trophozoite antigen/GS Ag (20ug/mL) or WB6 strain trophozoite antigen/WB6 Ag (20ug/mL). The cells were then incubated for 96 h at 37 °C and 5% CO₂. After 96 h, the cell culture supernatants were collected and used for measurement of IL-17 via ELISA using the Mouse IL-17 (homodimer) uncoated ELISA kit from Invitrogen according to the manufacturer’s protocol.

Preparation of intestinal tissue explants for antibody ELISA

At necropsy, 2 cm of tissue from the duodenum, jejunum and ileum sections of the small intestinal tract of naïve and infected BALB/c and C57BL/6 mice were excised and opened longitudinally. Each tissue sample was placed in a separate well in 1 mL pre-warmed RPMI 1640 medium, containing 1% FCS, 200 U/ml penicillin and 200 μg/ml streptomycin (all from PAA, Pasching, Austria) on a 48-well plate. The intestinal tissue explants were cultured for 24 h at 37 °C and 5% CO₂. Following incubation, the supernatants were collected and stored at −20 °C for further analysis via ELISA.

Antibody detection by enzyme-linked immunosorbent assay (ELISA)

Total IgA and IgG2b antibody titres in blood serum and intestinal tissue explant supernatants were quantified via sandwich ELISA. Briefly, 96-well flat bottom Maxisorp plates (Thermo Fischer Scientific, MA, USA) were coated with 50 μL goat anti-mouse IgA (Southern Biotech, AL, USA) and incubated overnight at 4 °C. Plates were washed using a Tecan Hydrospeed microplate washer and blocked with 200 μL 3% BSA in PBS for 1 h before 50 μL samples and standards were added. Plates were incubated with samples and standards for 2 h at room temperature, after which 50 μL goat anti-mouse AP-coupled IgA detection antibody (Southern Biotech, AL, USA) was added for 1 h. 50 μL phosphatase substrate was then added and plates were incubated for 30 min at 37 °C. To stop the enzymatic reaction, 25 μL 100 mM EDTA was added and the signal was measured at 405 nm minus reference wavelength 630 nm on a Biotek Synergy H1 Hybrid Reader.

Histopathological scoring and immunohistology

Formalin-fixed and paraffin-embedded 1-2 um sections of duodenum, jejunum and ileum were de-waxed and stained with hematoxylin and eosin (H&E) for overview and with fluorescently labelled anti-CD4 and anti-Foxp3 antibodies for the detection of T cells. CD4⁺ and CD4⁺Foxp3⁺ cells were counted along five villi per section. Enteritis was scored using H&E-stained sections as described before⁵⁷. All evaluations were performed blind.

Analysis of fecal microbiota via 16S rRNA-based qPCR

Fresh fecal pellets collected from naïve controls and mice infected with G. muris for two weeks were immediately snap-frozen in liquid nitrogen and stored at −80 °C until further processing. DNA was extracted from fecal samples using the innuPrep Stool DNA kit (AnalyticJena) as per the manufacturer’s instructions. For the qPCR analysis in brief, DNA was quantified by using Quant-iT PicoGreen reagent (Invitrogen, UK) and adjusted to 1 ng/μL. Then, the main bacterial groups abundant in the murine intestinal microbiota including Enterobacteria, Enterococci, Lactobacilli, Bifidobacteria, Bacteroides/Prevotella spp., Mouse Intestinal Bacteroides, Clostridium coccoides group, and Clostridium leptum group, as well as total Eubacterial loads were assessed by quantitative real-time PCR (qRT-PCR) with species-, genera-, or group-specific 16S rRNA gene primers (Tib MolBiol, Germany) as described previously⁵⁸. Numbers of 16S rRNA gene copies per nanogram DNA of each sample were determined.

High-throughput bacterial 16S rRNA Illumina sequencing of faecal microbiota

Genomic DNA was extracted from a total of 25 faecal samples collected form infected and uninfected mice of each line (i.e. 8 infected and 4 uninfected BALB/c, and 7 infected and 6 uninfected C57BL/6) as described above. High-throughput sequencing of prokaryotic 16S rRNA gene was performed on an Illumina MiSeq platform. Briefly, the V3-V4 region was PCR-amplified using universal primers⁵⁹, that contained the Illumina adapter overhang nucleotide sequences, using the Q5^® NEBNext hot start high-fidelity DNA polymerase (New England Biolabs), 5 ng/μL of template DNA and the following thermocycling protocol: 2 min at 98 °C, 20 cycles of 15 s at 98 °C – 30 s at 63 °C – 30 s at 72 °C, and a final elongation step of 5 min at 72 °C. Amplicons were purified using AMPure XP beads (Beckman Coulter) and set up for the index PCR using Q5^® NEBNext hot start high-fidelity DNA polymerase and Nextera XT index primers (Illumina), with thermocycling as follows: 3 min at 95 °C, 8 cycles of 30 s at 95 °C – 30 s at 55 °C – 30 s at 72 °C, and 5 min at 72 °C. The indexed samples were purified using AMPure XP beads, quantified using the Qubit dsDNA high sensitivity kit (Life Technologies), and equal amounts from each sample pooled. The resulting pooled library was quantified using the NEBNext library quantification kit (New England Biolabs) and sequenced using the v3 chemistry (2 × 300 bp paired-end reads, Illumina). The raw sequences are available from Mendeley (DOI: 10.17632/sj5cms7fky.1).

Statistical analysis and bioinformatics

Statistical analysis of FACS data was performed using GraphPad Prism software (La Jolla, CA, USA). Results are displayed as mean ± SD and significance is displayed as *p < 0.05, **p < 0.01, ***p < 0.001. Results were tested for normal distribution using the Shapiro-Wilk normality tests, followed by ANOVA or Kruskal-Wallis combined with Tukey’s or Dunn’s multiple comparison testing. For the correlation analysis between RORγt⁺ Treg:Th17 ratios, FLC16⁺ Th17 and Ki-67⁺ Th17 cell frequencies, Spearman’s rank correlation coefficient was calculated.

Paired-end demultiplexed Illumina reads were processed using the Quantitative Insights Into Microbial Ecology (QIIME2; 2019.4 release) software suite⁶⁰. Sequences were then quality filtered, dereplicated, chimeras were identified, and paired-end reads were merged in QIIME2 using DADA2⁶¹ with default settings. A phylogenetic tree was generated using the align-to-tree-mafft-fasttree pipeline in the q2-phylogeny plugin. Bray-Curtis dissimilarity between samples was calculated using core-metrics-phylogenetic method from the q2-diversity plugin. Classification of Operational Taxonomic Units (OTUs) was performed using a Naïve Bayes algorithm trained using sequences representing the bacterial V3-V4 rRNA region available from the SILVA database (; Silva_132)⁶², and the corresponding taxonomic classifications were obtained using the q2-feature-classifier plugin in QIIME2. The classifier was then used to assign taxonomic information to representative sequences of each OTU.

Statistical analyses were executed using the Calypso software⁶³ (cgenome.net/calypso/). For data normalisation, cumulative-sum scaling (CSS) was applied to the OTU table, followed by log2 transformation (CSS + log) to account for the non-normal distribution of taxonomic counts data. Samples from each mouse line were ordinated using unsupervised PCoA based on Bray-Curtis dissimilarities, and supervised CCA was then performed including infection status as explanatory variable. The same analyses were applied to samples from naïve BALB/c and C57BL/6 mice in order to address the differences in gut microbial community structure between both lines at the steady state. Following rarefaction of raw data, differences in microbial alpha diversity (Shannon diversity), richness and evenness were evaluated using ANOVA. Beta diversity was calculated using Bray-Curtis dissimilarity and differences in beta diversity were calculated using Analysis of Similarity (ANOSIM)⁶⁴. Differences in the composition of the microbiota between groups were assessed using the LEfSe workflow⁶⁵ on CSS + log transformed data, as well as negative binomial distribution (DESeq. 2)⁶⁶, the latter applied on not normalised, not rarefied datasets. Relative abundances were calculated on count data normalised by the total sum scaling (TSS) normalization method.

Data availability

The datasets used and analyzed in the current study are available from the corresponding author on reasonable request. The raw 16S rRNA data are available at Mendeley Data (doi: 10.17632/sj5cms7fky.1).

References

Torgerson, P. R. et al. World Health Organization estimates of the global and regional disease burden of 11 foodborne parasitic diseases, 2010: a data synthesis. PLoS Med. 12, e1001920; https://doi.org/10.1371/journal.pmed.1001920 (2015).
Article PubMed PubMed Central Google Scholar
Efstratiou, A., Ongerth, J. E. & Karanis, P. Waterborne transmission of protozoan parasites: Review of worldwide outbreaks - An update 2011-2016. Water Res. 114, 14–22, https://doi.org/10.1016/j.watres.2017.01.036 (2017).
Article PubMed Google Scholar
Lanata, C. F. et al. Global causes of diarrheal disease mortality in children <5 years of age: a systematic review. PloS ONE 8, e72788, https://doi.org/10.1371/journal.pone.0072788 (2013).
Article ADS CAS PubMed PubMed Central Google Scholar
Barash, N. R. et al. Giardia Colonizes and Encysts in High-Density Foci in the Murine Small Intestine. mSphere 2, e00343–16, https://doi.org/10.1128/mSphere.00343-16 (2017).
Article CAS PubMed PubMed Central Google Scholar
Einarsson, E., Ma’aye, S. & Svärd, S. G. An up-date on Giardia and giardiasis. Curr. Opin. Microbiol. 34, 47–52, https://doi.org/10.1016/j.mib.2016.07.019 (2016).
Article PubMed Google Scholar
Cotton, J. A., Beatty, J. K. & Buret, A. G. Host parasite interactions and pathophysiology in Giardia infections. Int. J. Parasitol. 41, 925–933, https://doi.org/10.1016/j.ijpara.2011.05.002 (2011).
Article CAS PubMed Google Scholar
Kohli, A. et al. Giardia duodenalis assemblage, clinical presentation and markers of intestinal inflammation in Brazilian children. Trans. R. Soc. Trop. Med. Hyg. 102, 718–725, https://doi.org/10.1016/j.trstmh.2008.03.002 (2008).
Article PubMed Google Scholar
Coklin, T. et al. Temporal changes in the prevalence and shedding patterns of Giardia duodenalis cysts and Cryptosporidium spp. oocysts in a herd of dairy calves in Ontario. Can. Vet. J. 51, 841–846 (2010).
CAS PubMed PubMed Central Google Scholar
Ebert, E. C. Giardia induces proliferation and interferon gamma production by intestinal lymphocytes. Gut 44, 342–346 (1999).
Article CAS PubMed PubMed Central Google Scholar
Saghaug, C. S. et al. Human Memory CD4+ T Cell Immune Responses against Giardia lamblia. Clin. Vaccine Immunol. 23, 11–18, https://doi.org/10.1128/CVI.00419-15 (2015).
Article CAS PubMed Google Scholar
Dann, S. M. et al. IL-17A promotes protective IgA responses and expression of other potential effectors against the lumen-dwelling enteric parasite Giardia. Exp. Parasitol. 156, 68–78, https://doi.org/10.1016/j.exppara.2015.06.003 (2015).
Article CAS PubMed PubMed Central Google Scholar
Dreesen, L. et al. Giardia muris infection in mice is associated with a protective interleukin 17A response and induction of peroxisome proliferator-activated receptor alpha. Infect. Immun. 82, 3333–3340, https://doi.org/10.1128/IAI.01536-14 (2014).
Article CAS PubMed PubMed Central Google Scholar
Paerewijck, O. et al. Interleukin-17 receptor A (IL-17RA) as a central regulator of the protective immune response against Giardia. Sci. Rep. 7, 8520, https://doi.org/10.1038/s41598-017-08590-x (2017).
Article ADS CAS PubMed PubMed Central Google Scholar
Cao, A. T., Yao, S., Elson, C. O. & Cong, Y. Th17 cells upregulate polymeric Ig receptor and intestinal IgA and contribute to intestinal homeostasis. J. Immunol. 189, 4666–4673, https://doi.org/10.4049/jimmunol.1200955 (2012).
Article CAS PubMed Google Scholar
Langford, T. D. et al. Central importance of Immunoglobulin A in host defense against Giardia spp. Infect. Immun. 70, 11–18 (2002).
Article CAS PubMed PubMed Central Google Scholar
Davids, B. J. et al. Polymeric immunoglobulin receptor in intestinal immune defense against the lumen-dwelling protozoan parasite Giardia. J. Immunol. 177, 6281–6290 (2006).
Article CAS PubMed Google Scholar
Shevach, E. M. Foxp3+ T Regulatory Cells: Still Many Unanswered Questions—A Perspective After 20 Years of Study. Front. Immunol. 9, 1048, https://doi.org/10.3389/fimmu.2018.01048 (2018).
Article CAS PubMed PubMed Central Google Scholar
Koch, M. A. et al. The transcription factor T-bet controls regulatory T cell homeostasis and function during type 1 inflammation. Nat. Immunol. 10, 595–602, https://doi.org/10.1038/ni.1731 (2009).
Article CAS PubMed PubMed Central Google Scholar
Wang, Y., Su, M. A. & Wan, Y. Y. An essential role of the transcription factor GATA-3 for the function of regulatory T cells. Immunity 35, 337–348, https://doi.org/10.1016/j.immuni.2011.08.012 (2011).
Article CAS PubMed PubMed Central Google Scholar
Sefik, E. et al. Individual intestinal symbionts induce a distinct population of RORγ+ regulatory T cells. Science 349, 993–997, https://doi.org/10.1126/science.aaa9420 (2015).
Article ADS CAS PubMed PubMed Central Google Scholar
Luu, M., Steinhoff, U. & Visekruna, A. A. functional heterogeneity of gut-resident regulatory T cells. Clin. Transl. Immunol. 6, e156, https://doi.org/10.1038/cti.2017.39 (2017).
Article CAS Google Scholar
Ohnmacht, C. et al. The microbiota regulates type 2 immunity through RORγt+ T cells. Science 349, 989–993, https://doi.org/10.1126/science.aac4263 (2015).
Article ADS CAS PubMed Google Scholar
Omenetti, S. & Pizarro, T. T. The Treg/Th17 axis: A dynamic balance regulated by the gut microbiome. Front. Immunol. 6, 639, https://doi.org/10.3389/fimmu.2015.00639 (2015).
Article CAS PubMed PubMed Central Google Scholar
Neumann, C. et al. c-Maf-dependent Treg cell control of intestinal Th17 cells and IgA establishes host–microbiota homeostasis. Nat. Immunol. 20, 471–481, https://doi.org/10.1038/s41590-019-0316-2 (2019).
Article CAS PubMed Google Scholar
Yang, B.-H. et al. Foxp3+ T cells expressing RORγt represent a stable regulatory T-cell effector lineage with enhanced suppressive capacity during intestinal inflammation. Mucosal Immunol. 9, 444–457, https://doi.org/10.1038/mi.2015.74 (2016).
Article CAS PubMed Google Scholar
Deenick, E. K., Hasbold, J. & Hodgkin, P. D. Switching to IgG3, IgG2b, and IgA is division linked and independent, revealing a stochastic framework for describing differentiation. J. Immunol. 163, 4707–4714 (1999).
CAS PubMed Google Scholar
Coffman, R. L., Lebman, D. A. & Shrader, B. Transforming growth factor beta specifically enhances IgA production by lipopolysaccharide-stimulated murine B lymphocytes. J. Exp. Med. 170, 1039–1044 (1989).
Article CAS PubMed Google Scholar
Pandiyan, P. et al. Microbiome dependent regulation of Tregs and Th17 cells in mucosa. Front. Immunol. 10, 426, https://doi.org/10.3389/fimmu.2019.00426 (2019).
Article CAS PubMed PubMed Central Google Scholar
Nakajima, A. et al. IgA regulates the composition and metabolic function of gut microbiota by promoting symbiosis between bacteria. J. Exp. Med. 215, 2019–2034, https://doi.org/10.1084/jem.20180427 (2018).
Article CAS PubMed PubMed Central Google Scholar
Belosevic, M., Faubert, G. M., Skamene, E. & MacLean, J. D. Susceptibility and resistance of inbred mice to Giardia muris. Infect. Immun. 44, 282–286 (1984).
CAS PubMed PubMed Central Google Scholar
Li, E., Zhou, P., Petrin, Z. & Singer, S. M. Mast cell-dependent control of Giardia lamblia infections in mice. Infect. Immun. 72, 6642–6649, https://doi.org/10.1128/IAI.72.11.6642-6649.2004 (2004).
Article CAS PubMed PubMed Central Google Scholar
Oberhuber, G., Kastner, N. & Stolte, M. Giardiasis: a histologic analysis of 567 cases. Scand. J. Gastroenterol. 32, 48–51 (1997).
Article CAS PubMed Google Scholar
Fink, M. Y. & Singer, S. M. The intersection of immune responses, microbiota and pathogenesis in giardiasis. Trends Parasitol. 33, 901–913, https://doi.org/10.1016/j.pt.2017.08.001 (2017).
Article CAS PubMed PubMed Central Google Scholar
Sano, T. et al. An IL-23R/IL-22 circuit regulates epithelial serum amyloid A to promote local effector Th17 responses. Cell 163, 381–393, https://doi.org/10.1016/j.cell.2015.08.061 (2015).
Article CAS PubMed PubMed Central Google Scholar
Schön, M. P. Mucosal T lymphocyte numbers are selectively reduced in integrin alpha E (CD103) deficient mice. J. Immunol. 162, 6641–6649 (1999).
PubMed Google Scholar
Mackay, L. K. Cutting edge: CD69 interference with sphingosine-1-phosphate receptor function regulates peripheral T cell retention. J. Immunol., https://doi.org/10.4049/jimmunol.1402256 (2015).
Article CAS PubMed Google Scholar
Xu, M. et al. c-MAF-dependent regulatory T cells mediate immunological tolerance to a gut pathobiont. Nature 554, 373–377, https://doi.org/10.1038/nature25500 (2018).
Article ADS CAS PubMed PubMed Central Google Scholar
Dann, S. M., Le, C. H. Y., Hanson, E. M., Ross, M. C. & Eckmann, L. Giardia infection of the small intestine induces chronic colitis in genetically-susceptible hosts. J. Immun. https://doi.org/10.4049/jimmunol.1700824 (2018).
Article PubMed Google Scholar
Barash, N. R., Maloney, J. G., Singer, S. M. & Dawson, S. C. Giardia alters commensal microbial diversity throughout the murine gut. Infect. Immun. 85, e00948–16, https://doi.org/10.1128/IAI.00948-16 (2017).
Article CAS PubMed PubMed Central Google Scholar
Karimi, K. et al. Lactobacillus reuteri-induced regulatory T cells protect against an allergic airway response in mice. Am. J. Respir. Crit. Care Med. 179, 186–193, https://doi.org/10.1164/rccm.200806-951OC (2009).
Article CAS PubMed Google Scholar
Atarashi, K. et al. Induction of colonic regulatory T cells by indigenous Clostridium species. Science 331, 337–341, https://doi.org/10.1126/science.1198469 (2011).
Article ADS CAS PubMed Google Scholar
Reynolds, L. A. et al. Commensal-pathogen interactions in the intestinal tract; lactobacilli promote infection with, and are promoted by, helminth parasites. Gut Microbes 5, 522–532, https://doi.org/10.4161/gmic.32155 (2014).
Article PubMed Google Scholar
Yordanova, I. A. et al. Micromanaging immunity in the murine host vs. the mosquito vector: microbiota-dependent immune responses to intestinal parasites. Front. Cell. Infect. Microbiol. 8, 308, https://doi.org/10.3389/fcimb.2018.00308 (2018).
Article CAS PubMed PubMed Central Google Scholar
Ivanov, I. I. et al. Induction of intestinal Th17 cells by Segmented Filamentous Bacteria. Cell 139, 485–498, https://doi.org/10.1016/j.cell.2009.09.033 (2009).
Article CAS PubMed PubMed Central Google Scholar
Atarashi, K. et al. Th17 cell induction by adhesion of microbes to intestinal epithelial cells. Cell 163, 367–380, https://doi.org/10.1016/j.cell.2015.08.058 (2015).
Article CAS PubMed PubMed Central Google Scholar
Singer, S. M. & Nash, T. E. The role of normal flora in Giardia lamblia infections in mice. J. Infect. Dis. 181, 1510–1512 (2000).
Article CAS PubMed Google Scholar
Paerewijck, O., Maertens, B., Gagnaire, A., de Bosscher, K. & Geldhof, P. Delayed development of the protective IL-17A response following a Giardia muris infection in neonatal mice. Sci. Rep. 9, 8959, https://doi.org/10.1038/s41598-019-45544-x (2019).
Article ADS CAS PubMed PubMed Central Google Scholar
Davis, C. P. & Savage, D. C. Habitat, succession, attachment, and morphology of segmented, filamentous microbes indigenous to the murine gastrointestinal tract. Infect. Immun. 10, 948–956 (1974).
CAS PubMed PubMed Central Google Scholar
Kumar, P. et al. Intestinal Interleukin-17 receptor signaling mediates reciprocal control of the gut microbiota and autoimmune inflammation. Immunity 44, 659–671, https://doi.org/10.1016/j.immuni.2016.02.007 (2016).
Article CAS PubMed PubMed Central Google Scholar
Suzuki, K. et al. Aberrant expansion of segmented filamentous bacteria in IgA-deficient gut. Proc. Natl. Acad. Sci. USA 101, 1981–1986 (2004).
Article ADS CAS PubMed PubMed Central Google Scholar
Manko, A. et al. Giardia co-infection promotes the secretion of antimicrobial peptides beta-defensin 2 and trefoil factor 3 and attenuates attaching and effacing bacteria-induced intestinal disease. PLoS ONE, e0178647; https://doi.org/10.1371/journal.pone.0178647 (2017).
Article PubMed PubMed Central Google Scholar
Manko, A. et al. A292 NLRP3-dependent production of antimicrobial peptides during co-infection with Giardia intestinalis and E. coli. J. Canad. Ass. Gastroenterol. 1, 506–507, https://doi.org/10.1093/jcag/gwy008.293 (2018).
Article Google Scholar
Daniels, C. W. & Belosevic, M. Serum antibody responses by male and female C57BL/6 mice infected with Giardia muris. Clin. Exp. Immunol. 97, 424–429 (1994).
Article CAS PubMed PubMed Central Google Scholar
Heyworth, M. F. & Pappo, J. Recognition of a 30,000 MW antigen of Giardia muris trophozoites by intestinal IgA from Giardia-infected mice. Immunology. 70, 535–539 (1990).
CAS PubMed PubMed Central Google Scholar
Roberts-Thomson, I. C., Svenes, D. P., Mahmoud, A. A. & Warren, K. S. Giardiasis in the mouse: an animal model. Gastroenterology 71, 57–61 (1976).
Article CAS PubMed Google Scholar
Strandmark, J. et al. Eosinophils are required to suppress Th2 responses in Peyer’s patches during intestinal infection by nematodes. Mucosal Immunol. 10, 661–672, https://doi.org/10.1038/mi.2016.93 (2017).
Article CAS PubMed Google Scholar
Rausch, S. et al. Establishment of nematode infection despite increased Th2 responses and immunopathology after selective depletion of Foxp3+ cells. Eur. J. Immunol. https://doi.org/10.1002/eji.200939644 (2009).
Article PubMed Google Scholar
Heimesaat, M. M. et al. Intestinal microbiota changes in mice lacking pituitary adenylate cyclase activating polypeptide (PACAP) - Bifidobacteria make the difference. Eur. J. Microbiol. Immunol. 7, 187–199, https://doi.org/10.1556/1886.2017.00021 (2017).
Article CAS Google Scholar
Klindworth, A. et al. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Res. 41, e1, https://doi.org/10.1093/nar/gks808 (2013).
Article CAS PubMed Google Scholar
Caporaso, J. G. et al. QIIME allows analysis of high-throughput community sequencing data. Nat. Methods 7, 335–336, https://doi.org/10.1038/nmeth.f.303 (2010).
Article CAS PubMed PubMed Central Google Scholar
Callahan, B. J. et al. DADA2: High-resolution sample inference from Illumina amplicon data. Nat. Methods 13, 581–583, https://doi.org/10.1038/nmeth.3869 (2016).
Article CAS PubMed PubMed Central Google Scholar
Quast, C. et al. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 41, D590–D596, https://doi.org/10.1093/nar/gks1219 (2013).
Article CAS PubMed Google Scholar
Zakrzewski, M. et al. Calypso: a user-friendly web-server for mining and visualizing microbiome–environment interactions. Bioinformatics 33, 782–783, https://doi.org/10.1093/bioinformatics/btw725 (2017).
Article CAS PubMed Google Scholar
Clarke, K. R. Non-parametric multivariate analyses of changes in community structure. Aust. J. Ecol. 18, 117–143, https://doi.org/10.1111/j.1442-9993.1993.tb00438.x (1993).
Article Google Scholar
Segata, N. et al. Metagenomic biomarker discovery and explanation. Genome Biol. 12, R60, https://doi.org/10.1186/gb-2011-12-6-r60 (2011).
Article PubMed PubMed Central Google Scholar
Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq. 2. Genome Biol. 15, 550 (2014).
Article PubMed PubMed Central Google Scholar

Download references

Acknowledgements

The authors would like to thank Yvonne Weber, Marion Müller, Bettina Sonnenburg, Christiane Palissa and Beate Anders for providing excellent technical support, as well as to the GRK2046 student assistant Martin Lamatsch for his help with sample collection and processing. Gratitudes are also extended to Gernot Reifenberger from the Charité for his assistance with the qPCR-based fecal microbiota analysis. This study was supported by the German Research Foundation (DFG) GRK2046 Research Training Program (CK, SH and SR) and a 3-year scholarship from the German Academic Exchange Service (DAAD) awarded to IAY. MMH received support from the German Federal Ministries of Education and Research (BMBF) in frame of the zoonoses research consortium PAC-Campylobacter (IP7/ 01KI1725D). AC is supported by a postdoctoral fellowship from Fundación Alfonso Martín Escudero (Madrid, Spain). Research in the CC laboratory is funded by grants from the Royal Society, the Horserase Betting Levy Board, the Biotechnology and Biological Sciences Research Council (BBSRC) and the Isaac Newton Trust.

Author information

Authors and Affiliations

Institute of Immunology, Centre for Infection Medicine, Freie Universität Berlin, Berlin, Germany
Ivet A. Yordanova, Susanne Hartmann & Sebastian Rausch
Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom
Alba Cortés & Cinzia Cantacessi
Unit 16 Mycotic and Parasitic Agents and Mycobacteria, Department of Infectious Diseases, Robert Koch-Institute, Berlin, Germany
Christian Klotz
iPATH.Berlin, Core Unit for Immunopathology for Experimental Models, Charité - University Medicine Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany
Anja A. Kühl
Institute of Microbiology, Infectious Diseases and Immunology Charité - University Medicine Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany
Markus M. Heimesaat

Authors

Ivet A. Yordanova
View author publications
You can also search for this author in PubMed Google Scholar
Alba Cortés
View author publications
You can also search for this author in PubMed Google Scholar
Christian Klotz
View author publications
You can also search for this author in PubMed Google Scholar
Anja A. Kühl
View author publications
You can also search for this author in PubMed Google Scholar
Markus M. Heimesaat
View author publications
You can also search for this author in PubMed Google Scholar
Cinzia Cantacessi
View author publications
You can also search for this author in PubMed Google Scholar
Susanne Hartmann
View author publications
You can also search for this author in PubMed Google Scholar
Sebastian Rausch
View author publications
You can also search for this author in PubMed Google Scholar

Contributions

I.A.Y. carried out the experiments and analyzed the data. A.C. performed the Illumina sequencing and analyzed the microbiota sequencing data. C.K., A.A.K., M.M.H. and C.C. provided valuable input into the study design, data analysis and critically reviewed and edited the manuscript. S.H. and S.R. designed the study and I.A.Y., S.R. and A.C. wrote the paper.

Corresponding author

Correspondence to Sebastian Rausch.

Ethics declarations

Competing interests

The authors declare no competing interests.

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Supplementary Information

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and permissions

About this article

Cite this article

Yordanova, I.A., Cortés, A., Klotz, C. et al. RORγt⁺ Treg to Th17 ratios correlate with susceptibility to Giardia infection. Sci Rep 9, 20328 (2019). https://doi.org/10.1038/s41598-019-56416-9

Download citation

Received: 16 July 2019
Accepted: 15 November 2019
Published: 30 December 2019
DOI: https://doi.org/10.1038/s41598-019-56416-9

This article is cited by

The dynamic shifts of IL-10-producing Th17 and IL-17-producing Treg in health and disease: a crosstalk between ancient "Yin-Yang" theory and modern immunology
- Huantian Cui
- Ning Wang
- Fudi Wang
Cell Communication and Signaling (2024)
Current Understanding of Giardia lamblia and Pathogenesis of Stunting and Cognitive Deficits in Children from Low- and Middle-Income Countries
- Lester Gutiérrez
- Luther Bartelt
Current Tropical Medicine Reports (2024)
A shotgun metagenomic analysis of the fecal microbiome in humans infected with Giardia duodenalis
- Brett A. McGregor
- Elham Razmjou
- Shahram Solaymani-Mohammadi
Parasites & Vectors (2023)
Probiotics in the management of Giardia duodenalis: an update on potential mechanisms and outcomes
- Nasrin Dashti
- Mitra Zarebavani
Naunyn-Schmiedeberg's Archives of Pharmacology (2021)

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.