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  • Oncogenomics
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Genome-wide allelotyping of 104 Finnish colorectal cancers reveals an excess of allelic imbalance in chromosome 20q in familial cases

Oncogene volume 22, pages 2206–2214 (2003)Cite this article

Abstract

We have allelotyped a series of 104 Finnish colorectal cancers (CRCs) using 372 polymorphic markers spaced, on average, at 10 cM intervals, and have made a comparison of the differences in the frequency of allelic imbalance (AI) between familial and sporadic cases. Differences in the frequency of allelic imbalance (loss of heterozygosity or amplification) at a number of loci were detected and these were evaluated through analysis of additional series of cancers using specific markers. The most consistent difference was observed at chromosome 20q13.1–13.3 characterized by a two fold difference between familial and nonfamilial disease in a total of 99 familial and 186 sporadic Finnish cases. This difference was not observed in a UK set of 67 familial and 96 sporadic CRCs. The genome-wide effort resulted in a large data set giving clues to the location of putative CRC predisposition genes in the genome. The approach provides an alternative strategy for detecting cancer predisposition genes solely reliant on the molecular analysis of single cases obviating the requirement to collect multiple samples from families.

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Acknowledgements

We thank Sini Marttinen and Mikko Aho. Most of the genetic marker analyses were conducted at the Finnish Genome Center. The study was supported by grants from the European Commission (QLG2-CT-2001-01861), Finnish Cancer Society, the Academy of Finland, Sigrid Juselius Foundation, Duodecim, Ida Montin Foundation, Jalmari and Rauha Ahokas Foundation, Emil Aaltonen Foundation, Nordisk Cancer Union, Paulo Foundation, Finnish Cultural Foundation, and Helsinki University Central Hospital. This work was carried out at the Center of Excellence in Disease Genetics of the Academy of Finland (Project No. 44870).

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Author notes

  1. Päivi Laiho and Tuija Hienonen: These authors contributed equally to the study

Authors and Affiliations

  1. Department of Medical Genetics, Biomedicum Helsinki, Haartmaninkatu 8, PO Box 63, University of Helsinki, FIN-00014, Finland

    Päivi Laiho, Tuija Hienonen, Auli Karhu, Reijo Salovaara, Virpi Launonen & Lauri A Aaltonen

  2. Molecular and Population Genetics Laboratory, Cancer Research UK, 44, Lincoln's Inn Fields, London, WC2A 3PX, UK

    Lara Lipton, Sarah Halford & Ian Tomlinson

  3. Colorectal Unit, Cancer Research UK, St Mark's Hospital, Watford Road, Harrow, HA1 3UJ, UK

    Lara Lipton & Huw JW Thomas

  4. Department of Medical Genetics, Haartman Institute and Helsinki University Central Hospital, University of Helsinki, Helsinki, FIN-00029, Finland

    Yan Aalto & Sakari Knuutila

  5. Department of Clinical Biochemistry, Molecular Diagnostic Laboratory, Aarhus University Hospital, Brendstrupgaardsvej, Aarhus N/Skejby, DK-8200, Denmark

    Karin Birkenkamp-Demtroder & Torben F Ørntoft

  6. Department of Clinical Genetics, Cancer Research UK, Guy's Hospital, London, SE1 9RT, UK

    Shirley Hodgson

  7. Department of Pathology, Haartman Institute, Haartmaninkatu 3, PO Box 21, University of Helsinki, Helsinki, FIN-00014, Finland

    Reijo Salovaara

  8. Department of Surgery, Jyväskylä Central Hospital, Keskussairaalantie 19, Jyvä, FIN-40620, skylä, Finland

    Jukka-Pekka Mecklin

  9. Second Department of Surgery, Helsinki University Central Hospital, PO Box 262, Helsinki, FIN-00029, Finland

    Heikki Järvinen

  10. Section of Cancer Genetics, Institute of Cancer Research, 15 Cotswold Road, Sutton, SM2 5NG, Surrey, UK

    Richard Houlston

  11. Department of Oncology, Helsinki University Central Hospital, PO Box 180, Helsinki, FIN-00029, Finland

    Lauri A Aaltonen

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  1. Päivi Laiho
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  2. Tuija Hienonen
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  8. Shirley Hodgson
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  9. Reijo Salovaara
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  12. Sakari Knuutila
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  13. Sarah Halford
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  15. Ian Tomlinson
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  16. Virpi Launonen
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  17. Richard Houlston
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  18. Lauri A Aaltonen
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Corresponding author

Correspondence to Lauri A Aaltonen.

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Laiho, P., Hienonen, T., Karhu, A. et al. Genome-wide allelotyping of 104 Finnish colorectal cancers reveals an excess of allelic imbalance in chromosome 20q in familial cases. Oncogene 22, 2206–2214 (2003). https://doi.org/10.1038/sj.onc.1206294

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