Engineering molecular recognition in alkane oxidation catalysed by cytochrome P450cam
Abstract
The activity, selectivity and coupling efficiency of alkane oxidation by the monooxygenase enzyme cytochrome P450cam can be enhanced by engineering the active site topology to accommodate different alkanes: reducing the active site volume by the Y96F–V247L double mutations resulted in four-fold higher activity for the oxidation of hexane (1) than 3-methylpentane (2) while the larger active site of the Y96A–V247A double mutant gave rise to a two-fold preference for 2 over 1.
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