Issue 3, 2003
From the journal:

Analyst

Study of carbon nanotubes–HRP modified electrode and its application for novel on-line biosensors

Katsunobu Yamamoto,a   Guoyue Shi,*b   Tianshu Zhou,c   Fan Xu,a   Jiming Xu,b   Takeshi Kato,a   Ji-Ye Jind  and  Litong Jinb  

* Corresponding authors

a Laboratory of Research and Development, BAS Inc., 1-36-6, Oshiage, Sumida-Ku, Tokyo, Japan

b Department of Chemistry, East China Normal University, Shanghai 200062, P. R. of China

c Department of Environmental Science, East China Normal University, Shanghai 200062, P. R. of China

d Instrumental Analysis Center, Gifu University, Yanagido, Gifu 501-1193, Japan

Abstract

In this paper, multi-walled carbon nanotubes (MWCNTs) were successfully immobilized on the surface of a glassy carbon electrode by mixing with horse-radish peroxidase (HRP). The electrochemical behavior of H2O2 was also studied with the MWCNTs–HRP modified electrode as a working electrode. The MWCNTs–HRP modified electrode showed excellent response of reduction current for the determination of H2O2 at the potential of −300 mV (vs. Ag/AgCl). We assembled the MWCNTs–HRP modified electrode in a thin-layer flow cell and the H2O2 solution was continuously introduced into the cell with a syringe pump. We optimized the sensitivity of the H2O2 sensor by adjusting the working potential and the pH of the buffer solution. The peak current increased linearly with the concentration of H2O2 in the range 3.0 × 10−7 ∼ 2.0 × 10−4 mol L−1. The detection limit is 1.0 × 10−7 mol L−1 (S/N = 3). The interferences from ascorbic acid, uric acid and other electroactive substances can be greatly excluded since the sensor can be operated at −300 mV. Stability and reproducibility of the MWCNTs–HRP chemically modified electrode were also studied in this paper. Fabricated with glucose and lactate oxidase, the MWCNTs–HRP electrode was also applied to prepare the on-line glucose and lactate biosensors because of the high sensitivity for the determination of H2O2.

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Article information

DOI
https://doi.org/10.1039/B209698F
Article type
Paper
Submitted
03 Oct 2002
Accepted
13 Feb 2003
First published
25 Feb 2003

Analyst, 2003,128, 249-254

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Study of carbon nanotubes–HRP modified electrode and its application for novel on-line biosensors

K. Yamamoto, G. Shi, T. Zhou, F. Xu, J. Xu, T. Kato, J. Jin and L. Jin, Analyst, 2003, 128, 249 DOI: 10.1039/B209698F

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