Issue 7, 2003
From the journal:

Analyst

Metabonomic analysis of mouse urine by liquid-chromatography-time of flight mass spectrometry (LC-TOFMS): detection of strain, diurnal and gender differences

Robert Plumb,a   Jennifer Granger,a   Chris Stumpf,a   Ian D. Wilson,*b   Julie A. Evansb  and  Eva M. Lenzb  

* Corresponding authors

a Life Sciences Research and Development, Waters Corporation, 34 Maple St, Milford, MA 01757, USA

b Department of Drug Metabolism and Pharmacokinetics, AstraZenca, Mereside, Alderley Park, Macclesfield, Cheshire, UK

Abstract

The application of HPLC-MS combined with principal components analysis (PCA) to the metabonomic analysis of mouse urine is demonstrated. Urine samples from three strains of mouse were analysed by gradient HPLC-MS combined with positive and negative electrospray time-of-flight mass spectrometry. Analysis of the resulting data using PCA enabled the samples to be discriminated between on the basis of gender, strain and diurnal variation. These preliminary results suggest that HPLC-MS-based approaches may have a useful role in metabonomic analysis that complements existing approaches.

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Article information

DOI
https://doi.org/10.1039/B304296K
Article type
Communication
Submitted
17 Apr 2003
Accepted
04 Jun 2003
First published
19 Jun 2003

Analyst, 2003,128, 819-823

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Metabonomic analysis of mouse urine by liquid-chromatography-time of flight mass spectrometry (LC-TOFMS): detection of strain, diurnal and gender differences

R. Plumb, J. Granger, C. Stumpf, I. D. Wilson, J. A. Evans and E. M. Lenz, Analyst, 2003, 128, 819 DOI: 10.1039/B304296K

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