Electrochemical biosensors utilising electron transfer in heme proteins immobilised on Fe3O4 nanoparticles

Dongfang Cao; Pingli He; Naifei Hu

doi:10.1039/B308242C

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Electrochemical biosensors utilising electron transfer in heme proteins immobilised on Fe₃O₄ nanoparticles†

Dongfang Cao,^a Pingli He^a and Naifei Hu*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, Beijing Normal University, Beijing 100875, China
E-mail: hunaifei@bnu.edu.cn

Abstract

Fe₃O₄ nanoparticles cast on pyrolytic graphite (PG) electrodes were used to immobilize hemoglobin (Hb), myoglobin (Mb) and horseradish peroxidase (HRP). The Fe₃O₄ nanoparticles provided a favorable microenvironment for the proteins to directly transfer electrons with electrodes. The protein–Fe₃O₄ films were used to electrochemically catalyze the reduction of oxygen, trichloroacetic acid, nitrite and hydrogen peroxide, and showed a potential applicability in fabricating biosensors. Transmission electron microscopy (TEM), UV–visible absorption and reflectance absorption infrared (RAIR) spectroscopy, and cyclic and square wave voltammetry, were used to characterize the films.

Download options Please wait...

Supplementary files

Characterization of protein-Fe ₃O ₄ films DOC (164K)

Article information

DOI: https://doi.org/10.1039/B308242C
Article type: Paper
Submitted: 18 Jul 2003
Accepted: 15 Sep 2003
First published: 29 Sep 2003

Download Citation

Analyst, 2003,128, 1268-1274

Permissions

Request permissions

Electrochemical biosensors utilising electron transfer in heme proteins immobilised on Fe₃O₄ nanoparticles

D. Cao, P. He and N. Hu, Analyst, 2003, 128, 1268 DOI: 10.1039/B308242C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Analyst