Issue 6, 2004
From the journal:

Lab on a Chip

Micro patterning of cell and protein non-adhesive plasma polymerized coatings for biochip applications

S. Bouaidat,*ab   C. Berendsen,a   P. Thomsen,a   S. Guldager Petersen,a   A. Wolffb  and  J. Jonsmanna  

* Corresponding authors

a Scandinavian Micro Biodevices ApS (SMB), Gammelgårdsvej 87C, DK-3520 Farum, Denmark
E-mail: sab@smb.dk

b Department of Micro and Nanotechnology (MIC), The Technical University of Denmartk, Building 345east, DK-2800 Kgs., Lyngby, Denmark

Abstract

Micro scale patterning of bioactive surfaces is desirable for numerous biochip applications. Polyethyleneoxide-like (PEO-like) coating with non-fouling functionality has been deposited using low frequency AC plasma polymerization. The non-fouling properties of the coating were tested with human cells (HeLa) and fluorescence labeled proteins (isothiocyanate-labeled bovine serum albumin, i.e. FITC-BSA). The PEO-like coatings were fabricated by plasma polymerization of 12-crown-4 (ppCrown) with plasma polymerized hexene (ppHexene) as adhesion layer. The coatings were micro patterned using conventional cleanroom photolithography and lift-off. Single cell arrays showed sharp contrast in cell adhesion between the untreated glass surface and the ppCrown layer. Similarly, proteins adsorbed selectively to untreated glass but not to ppCrown. The simplicity of the lift-off technique and the sturdiness and versatility of the plasma-polymerized coatings, make this technology highly suitable for bio-MEMS and biochip applications, where patterned high contrast non-fouling surfaces are needed.

Graphical abstract: Micro patterning of cell and protein non-adhesive plasma polymerized coatings for biochip applications

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Article information

DOI
https://doi.org/10.1039/B406285J
Article type
Paper
Submitted
27 Apr 2004
Accepted
15 Jul 2004
First published
04 Oct 2004

Lab Chip, 2004,4, 632-637

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Micro patterning of cell and protein non-adhesive plasma polymerized coatings for biochip applications

S. Bouaidat, C. Berendsen, P. Thomsen, S. G. Petersen, A. Wolff and J. Jonsmann, Lab Chip, 2004, 4, 632 DOI: 10.1039/B406285J

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