Issue 11, 2010
From the journal:

Lab on a Chip

Handheld mechanical cell lysis chip with ultra-sharp silicon nano-blade arrays for rapid intracellularproteinextraction

Sung-Sik Yun,ab   Sang Youl Yoon,c   Min-Kyung Song,d   Sin-Hyeog Im,d   Sohee Kim,ab   Jong-Hyun Leeabc  and  Sung Yang*abc  

* Corresponding authors

a Department of Nanobio Materials and Electronics, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro (Oryong-dong), Buk-gu, Gwangju, Republic of Korea 500-712
E-mail: syang@gist.ac.kr
Fax: +82-62-970-2384
Tel: +82-62-970-2407

b School of Information and Mechatronics, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro (Oryong-dong), Buk-gu, Gwangju, Republic of Korea 500-712

c Graduate Program of Medical System Engineering, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro (Oryong-dong), Buk-gu, Gwangju, Republic of Korea 500-712

d Department of Life Sciences, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro (Oryong-dong), Buk-gu, Gwangju, Republic of Korea 500-712

Abstract

This paper presents a handheld mechanical cell lysis chip with ultra-sharp nano-blade arrays fabricated by simple and cost effective crystalline wet etching of (110) silicon. The ultra-sharp nano-blade array is simply formed by the undercutting of (110) silicon during the crystalline wet etching process. Cells can be easily disrupted by the silicon nano-blade array without the help of additional reagents or electrical sources. Based on the bench-top test of the proposed device, a handheld mechanical cell lysis chip with the nano-blade arrays is designed and fabricated for direct connection to a commercial syringe. The direct connection to a syringe provides rapid cell lysis, easy handling, and minimization of the lysate dead volume. The protein concentration in the cell lysate obtained by the proposed lysis chip is quantitatively comparable to the one prepared by a conventional chemical lysis method.

Graphical abstract: Handheld mechanical cell lysis chip with ultra-sharp silicon nano-blade arrays for rapid intracellular protein extraction

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Article information

DOI
https://doi.org/10.1039/B925244D
Article type
Paper
Submitted
02 Dec 2009
Accepted
09 Feb 2010
First published
16 Mar 2010

Lab Chip, 2010,10, 1442-1446

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Handheld mechanical cell lysis chip with ultra-sharp silicon nano-blade arrays for rapid intracellular protein extraction

S. Yun, S. Y. Yoon, M. Song, S. Im, S. Kim, J. Lee and S. Yang, Lab Chip, 2010, 10, 1442 DOI: 10.1039/B925244D

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