Issue 5, 2010
From the journal:

Analytical Methods

Microfluidic colourimetric chemical analysis system: Application to nitrite detection

Vincent J. Sieben,*ab   Cedric F. A. Floquet,b   Iain R. G. Ogilvie,b   Matthew C. Mowlemb  and  Hywel Morgana  

* Corresponding authors

a Nanoscale systems integration group, University of Southampton, Southampton, UK
E-mail: hm@ecs.soton.ac.uk, v.sieben@noc.soton.ac.uk
Fax: +44 (0)23 8059 3029
Tel: +44 (0)23 8059 3330

b National Oceanography Centre, University of Southampton, Southampton, UK
E-mail: mcm@noc.soton.ac.uk
Fax: +44 (0)23 8059 6149
Tel: +44 (0)23 8059 6379

Abstract

The design, fabrication, analysis and characterisation of a continuous flow, microfluidic absorption cell and detection system is described. A low cost optical illumination and detection method is integrated with a simple microfluidic system. Nitrite is detected at nM concentration using the Griess reaction. The device is based on a novel optical absorption scheme with microfluidic channels made from tinted PMMA. An absorbance path length of 25 mm enables nitrite to be detected from 50 nM to 10 μM with a limit of detection of 14 nM. The system was used to characterise the reaction kinetics and maximum sample resolution. In this report the system is used to detect nitrite, but it could equally be used to assay a range of different chemistries using colourimetric methods. The system provides a cost-effective, simple system that could be deployed in a range of scenarios for environmental monitoring.

Graphical abstract: Microfluidic colourimetric chemical analysis system: Application to nitrite detection

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Article information

DOI
https://doi.org/10.1039/C002672G
Article type
Paper
Submitted
04 Nov 2009
Accepted
08 Feb 2010
First published
19 Feb 2010

Anal. Methods, 2010,2, 484-491

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Microfluidic colourimetric chemical analysis system: Application to nitrite detection

V. J. Sieben, C. F. A. Floquet, I. R. G. Ogilvie, M. C. Mowlem and H. Morgan, Anal. Methods, 2010, 2, 484 DOI: 10.1039/C002672G

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