Issue 23, 2010
From the journal:

Organic & Biomolecular Chemistry

Structural characterization of the core region from the lipopolysaccharide of the haloalkaliphilic bacterium Halomonas alkaliantarctica strain CRSS

Giuseppina Pieretti,a   Sara Carillo,a   Barbara Nicolaus,b   Annarita Poli,b   Rosa Lanzetta,a   Michelangelo Parrillia  and  Maria Michela Corsaro*a  

* Corresponding authors

a Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia 4, Italy
E-mail: corsaro@unina.it
Fax: +39 081674393
Tel: +39 081674149

b Istituto di Chimica Biomolecolare ICB-CNR, Via Campi Flegrei 34, Italy

Abstract

Halophilic and halotolerant Gram-negative bacteria are microorganisms which thrive in high salt environments. LPS are the major components of their outer leaflet, nevertheless very little is known about the role of this molecules in the adaptation mechanisms of extremophiles. Recently we determined the O-chain repeating unit structure of the LPS from Halomonas alkaliantarctica strain CRSS, an haloalkaliphilic Gram-negative bacterium isolated from salt sediments of a saline lake in Cape Russell in Antarctic continent. The polysaccharide is constituted of the trisaccharidic repeating unit: →3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-α-L-Rhap-(1→. In this paper we report the complete core LPS structure from this bacterium. The LPS was hydrolyzed both under mild acid and strong alkaline conditions. The MALDI spectra showed the presence of two glycoforms. The most abundant was recovered after HPAEC purification of the alkaline hydrolyzed product and was characterized by means of 2D-NMR spectroscopy. A comparison of the MALDI-PSD spectra of the two glycoforms suggested that the branched heptose was not stoichiometrically substituted.

Graphical abstract: Structural characterization of the core region from the lipopolysaccharide of the haloalkaliphilic bacterium Halomonas alkaliantarctica strain CRSS

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Article information

DOI
https://doi.org/10.1039/C0OB00516A
Article type
Paper
Submitted
29 Jul 2010
Accepted
31 Aug 2010
First published
04 Oct 2010

Org. Biomol. Chem., 2010,8, 5404-5410

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Structural characterization of the core region from the lipopolysaccharide of the haloalkaliphilic bacterium Halomonas alkaliantarctica strain CRSS

G. Pieretti, S. Carillo, B. Nicolaus, A. Poli, R. Lanzetta, M. Parrilli and M. M. Corsaro, Org. Biomol. Chem., 2010, 8, 5404 DOI: 10.1039/C0OB00516A

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