Issue 8, 2011
From the journal:

Food & Function

Cranberry phytochemicals inhibit glycation of human hemoglobin and serum albumin by scavenging reactive carbonyls†

Haiyan Liu,a   Hanwei Liu,a   Wei Wang,a   Christina Khoo,b   James Taylorc  and  Liwei Gu*a  

* Corresponding authors

a Department of Food Science and Human Nutrition, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida, United States
E-mail: lgu@ufl.edu
Fax: +1 (352)392-9467
Tel: +1 (352) 392-1991 ex 210

b Ocean Spray Cranberries, Inc., One Ocean Spray Drive, Lakeville-Middleboro, MA, United States

c College of Pharmacy, University of Florida, Gainesville, Florida, United States

Abstract

Protein glycation caused by sugars and reactive carbonyls is a contributing factor to diabetic complications, aging, and other chronic diseases. The objective of this study was to investigate the inhibitory effects of cranberry phytochemicals on protein glycation. Cranberries, purified to yield sugar-free phytochemical powder, were fractionated into ethyl acetate and water fractions. Water fraction was further separated into water fraction I, II, and III on a Sephadex LH-20 column. Cranberry phytochemical powder and its fractions significantly inhibited the formation of glycated hemoglobin. The concentrations of cranberry phytochemicals required to inhibit 50% of albumin glycation (EC50) in albumin-glucose assay were lower than that of aminoguanidine except for water fraction I. Cranberry phytochemicals inhibited glycation of human serum albumin mediated by methylglyoxal, but the EC50 were higher than that of aminoguanidine. Carbonyl scavenging assay showed that water fraction II scavenged 89.3% of methylglyoxal at 6 h of reaction. Fractions enriched with procyanidins showed higher antiglycation activities, suggesting procyanidins were the major active components. The hypothesis whether cranberry procyanidins scavenged reactive carbonyls by forming adducts was tested. Epicatechin was used as a model compound to react with methylglyoxal and glyoxal at pH 7.4. Five adducts were detected and their structures were tentatively identified using HPLC-ESI-MS/MS.

Graphical abstract: Cranberry phytochemicals inhibit glycation of human hemoglobin and serum albumin by scavenging reactive carbonyls

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Article information

DOI
https://doi.org/10.1039/C1FO10087D
Article type
Paper
Submitted
29 May 2011
Accepted
11 Jul 2011
First published
08 Aug 2011

Food Funct., 2011,2, 475-482

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Cranberry phytochemicals inhibit glycation of human hemoglobin and serum albumin by scavenging reactive carbonyls

H. Liu, H. Liu, W. Wang, C. Khoo, J. Taylor and L. Gu, Food Funct., 2011, 2, 475 DOI: 10.1039/C1FO10087D

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