A BODIPY-based fluorescent dye for mitochondria in living cells, with low cytotoxicity and high photostability

Si Zhang; Tong Wu; Jiangli Fan; Zhiyong Li; Na Jiang; Jingyun Wang; Bairui Dou; Shiguo Sun; Fengling Song; Xiaojun Peng

doi:10.1039/C2OB26911B

A BODIPY-based fluorescent dye for mitochondria in living cells, with low cytotoxicity and high photostability†

Si Zhang,^a Tong Wu,^a Jiangli Fan,^a Zhiyong Li,^a Na Jiang,^a Jingyun Wang,*^b Bairui Dou,^b Shiguo Sun,^a Fengling Song^a and Xiaojun Peng*^a

Author affiliations

* Corresponding authors

^a State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian, P.R. China
E-mail: pengxj@dlut.edu.cn
Fax: +86-411-84986306
Tel: +86-411-84986306

^b A School of Life Science & Biotechnology, Dalian University of Technology, 2 Linggong Road, Dalian 116024, P.R. China
E-mail: wangjingyun@dl.cn

Abstract

A BODIPY-based dye, OBEP, has been developed to act as a mitochondrial fluorescence probe. This dye is of high stability, low toxicity and insensitive in a pH range as wide as pH 2–10. Its uptake into mitochondria is independent of mitochondrial membrane potential in living cells. OBEP can label swollen mitochondria resulting from different degrees of cell damage in light and resist fading even after 12 h of incubation.

Organic & Biomolecular Chemistry

A BODIPY-based fluorescent dye for mitochondria in living cells, with low cytotoxicity and high photostability†

Abstract

Supplementary files

Article information

Download Citation

Permissions

A BODIPY-based fluorescent dye for mitochondria in living cells, with low cytotoxicity and high photostability

Social activity

Search articles by author

Spotlight

Advertisements