Issue 23, 2013
From the journal:

Analytical Methods

Fluorescence spectroscopy and multi-way techniques. PARAFAC†

Kathleen R. Murphy,*a   Colin A. Stedmon,b   Daniel Graeberc  and  Rasmus Brod  

* Corresponding authors

a University of New South Wales, Water Research Centre, Sydney, Australia
E-mail: krm@unsw.edu.au
Fax: +61 2 9313 8624
Tel: +61 2 9385 4601

b Technical University of Denmark, National Institute for Aquatic Resources, Charlottenlund, Denmark
E-mail: cost@aqua.dtu.dk

c Aarhus University, Department of Bioscience, Silkeborg, Denmark
E-mail: dgr@dmu.dk

d University of Copenhagen, Dept. Food Science, Frederiksberg, Denmark
E-mail: rb@life.ku.dk

Abstract

PARAllel FACtor analysis (PARAFAC) is increasingly used to decompose fluorescence excitation emission matrices (EEMs) into their underlying chemical components. In the ideal case where fluorescence conforms to Beers Law, this process can lead to the mathematical identification and quantification of independently varying fluorophores. However, many practical and analytical hurdles stand between EEM datasets and their chemical interpretation. This article provides a tutorial in the practical application of PARAFAC to fluorescence datasets, demonstrated using a dissolved organic matter (DOM) fluorescence dataset. A new toolbox for MATLAB is presented to support improved visualisation and sensitivity analyses of PARAFAC models in fluorescence spectroscopy.

Graphical abstract: Fluorescence spectroscopy and multi-way techniques. PARAFAC

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C3AY41160E
Article type
Tutorial Review
Submitted
12 Jul 2013
Accepted
09 Sep 2013
First published
10 Sep 2013
This article is Open Access
Creative Commons BY-NC license

Anal. Methods, 2013,5, 6557-6566

Permissions

Request permissions

Fluorescence spectroscopy and multi-way techniques. PARAFAC

K. R. Murphy, C. A. Stedmon, D. Graeber and R. Bro, Anal. Methods, 2013, 5, 6557 DOI: 10.1039/C3AY41160E

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements