Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC

Kandasamy Selvam; Muthusamy Govarthanan; Seralathan Kamala-Kannan; Munisamy Govindharaju; Balakrishnan Senthilkumar; Thangasamy Selvankumar; Arumugam Sengottaiyan

doi:10.1039/C4RA00066H

Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC

Kandasamy Selvam,†^a Muthusamy Govarthanan,†^bc Seralathan Kamala-Kannan,^c Munisamy Govindharaju,^d Balakrishnan Senthilkumar,*^a Thangasamy Selvankumar*^bd and Arumugam Sengottaiyan^b

Author affiliations

* Corresponding authors

^a Centre for Biotechnology, Muthayammal College of Arts and Science, Rasipuram, Namakkal – 637 408, Tamil Nadu, India
E-mail: nbsenthilkumar@gmail.com

^b PG & Research Department of Biotechnology, Mahendra Arts and Science College, Kalippatti, Namakkal – 637501, Tamil Nadu, India
E-mail: t_selvankumar@yahoo.com
Tel: +91-9443470394

^c Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan – 570752, South Korea

^d Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli – 620 024, Tamilnadu, India

Abstract

The aim of this study was to assess the mixed combination of coffee pulp waste (CPW) and pineapple waste (PW) residues for cellulase production using newly isolated Acinetobacter sp. TSK-MASC in solid state fermentation. Response surface methodology based Box–Behnken design (BBD) was employed to optimize variables such as pH, incubation time, concentrations of CPW and PW. The BBD design investigation showed a sound adjustment of the quadratic model with the experimental statistics. Statistics based 3-D plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and culture parameters. The higher production (888 U mL⁻¹) was achieved after 60 h of incubation with 3.0 g L⁻¹ of CPW and PW at pH 7.0.

Article information

https://doi.org/10.1039/C4RA00066H

Article type

Paper

Submitted

04 Jan 2014

Accepted

30 Jan 2014

First published

30 Jan 2014

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RSC Adv., 2014,4, 13045-13051

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Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC

K. Selvam, M. Govarthanan, S. Kamala-Kannan, M. Govindharaju, B. Senthilkumar, T. Selvankumar and A. Sengottaiyan, RSC Adv., 2014, 4, 13045 DOI: 10.1039/C4RA00066H

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Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC

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Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC

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